An in vivo study on bone formation behavior of microporous granular calcium phosphate

Abstract

This study was developed based on in vivo investigation of microporous granular biomaterials based on calcium phosphates, involving matrices of β-tricalcium phosphate (β-TCP), hydroxyapatite (HA), biphasic compositions of both phases and a control group. The physicochemical characterization of materials was carried out by X-Ray diffraction (DRX) and mercury porosimetry. Biodegradability, bioactivity and neoformation processes were investigated by Raman spectroscopy, scanning electron microscopy (SEM) and polarized light conducted on biopsies obtained from in vivo tests for periods of 90 and 180 days. These were performed to evaluate the behavior of granular microporous compositions in relation to bone neoformation. Through the performance obtained from in vivo assays, excellent osseointegration and bone tissue neoformation were observed. The results are encouraging and show that the microporous granular biomaterials of HA, β-TCP and biphasic compositions show similar results with perfect osseointegration. Architectures simulating a bone structure can make the difference between biomaterials for bone tissue replacement and repair.

Photopolymerization for filling porous ceramic matrix: Improvement of mechanical properties and drug delivering behavior

“…The dried polymer matrix was easily removed and the ceramic surface analyzed by Raman. This technique is useful technique to observe the phases dissolved in the surface of the scaffolds the in vitro assay similar to to the study realized by in in vivo assays . The Raman spectra obtained from the surface of the ceramic portion of the scaffold show signals due to the vibration modes of from psW , and , from β‐TCP (Fig.…”

Hydroxyapatite-Based Materials for Potential Use in Bone Tissue Infections

“…HA is considered to be the gold standard in bone tissue regeneration. In clinical practice, it is used in the form of powders or granules as filler for bone replacement or for repair of post-resection defects [4,5]. HA is also successfully used as a coating material for metallic implants due to its bioactivity and favourable effects on the osseointegration process [6].…”

Zein/gelatin/nanohydroxyapatite nanofibrous scaffolds are biocompatible and promote osteogenic differentiation of human periodontal ligament stem cells

“…Ideally, a biomaterial scaffold should best mimic the natural ECM in terms of compositional and structural features. 21,24 In this study, the electrospinning technique was applied to produce three-dimensional zein/gelatin/nHAp nanofibrous membranes with a porous interconnected nanostructure. The water contact angle of the membranes had a direct correlation with the mass ratio of nHAp, and the surface wettability was improved after the nHAp content was increased because of the hydrophilic nature of nHAp.…”

“…It can enhance the intrinsic strength and biological activity of other biological materials and thus reduce their negative effects. [21][22][23] Thus, nHAp is considered to be the most promising material in bone regeneration.…”