An in vivo proteomic study of the interaction between Salmonella Typhimurium and porcine ileum mucosa

Collado-Romero, Melania; Martins, Rodrigo Prado; Arce, Cristina; Moreno, A.; Lucena, Carlos; Carvajal, Ana; Garrido, Juan J.

doi:10.1016/j.jprot.2012.01.001

Cited by 32 publications

(24 citation statements)

References 67 publications

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“…These authors found villin abundance to decrease in infected pigs and associated this finding with membrane ruffling and microvilli denuding [34]. Colonising germ free pigs with either nonpathogenic E. coli or L. fermentum resulted in actin and actin proteins associated with remodelling, including tropomyosin, to increase in abundance in the gut tissues of the pigs colonised with E. coli.…”

Section: Differentially Abundant Proteinsmentioning

confidence: 92%

The effect of microbial challenge on the intestinal proteome of broiler chickens

O'Reilly¹,

Burchmore²,

Eckersall³

et al. 2013

Farm Animal Proteomics 2013

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Background: In poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. Uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. The exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. It rarely causes mortality but effects an immunogenic stimulation sufficient enough to cause reduced and uneven growth that is reflective of a challenging growing environment.

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Section: Differentially Abundant Proteinsmentioning

confidence: 92%

The effect of microbial challenge on the intestinal proteome of broiler chickens

O'Reilly¹,

Burchmore²,

Eckersall³

et al. 2013

Farm Animal Proteomics 2013

View full text Add to dashboard Cite

show abstract

“…4C) and cecum (data not shown) in S. Typhimuriuminfected ducks as compared to the uninfected ducks. It is interesting to note that IL-17A mRNA expression is unchanged in the cecum of chickens orally infected with S. Enteritidis (Matulova et al, 2012), and significantly downregulated in the ileum mucosa of pigs orally infected with S. Typhimurium (Collado-Romero et al, 2012). By contrast, increased expression of IL-17A transcript is detected in the cecum of chickens orally infected with S. Enteritidis (Crhanova et al, 2011), and in the ceca of streptomycinpretreated mice orally infected with S. Typhimurium (Godinez et al, 2008).…”

Section: Quantitative Analysis Of Duil-17a and Duil-17f Mrna Expressimentioning

confidence: 98%

Molecular cloning, characterization and mRNA expression of duck interleukin-17F

Kim

Fernandez

Diaz

et al. 2015

Veterinary Immunology and Immunopathology

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“…In pigs infections such as those caused by Salmonella enterica serovar Thyphimurium increase the abundance of cytoskeletal proteins such as actin in the intestinal proteome, while proteins associated with actin function show variable responses [34], in keeping with the findings in the challenged birds. These authors found villin abundance to decrease in infected pigs and associated this finding with membrane ruffling and microvilli denuding [34].…”

Section: Discussionmentioning

confidence: 70%

The effect of microbial challenge on the intestinal proteome of broiler chickens

et al. 2016

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BackgroundIn poultry production intestinal health and function is paramount to achieving efficient feed utilisation and growth. Uncovering the localised molecular mechanisms that occur during the early and important periods of growth that allow birds to grow optimally is important for this species. The exposure of young chicks to used litter from older flocks, containing mixed microbial populations, is a widely utilised model in poultry research. It rarely causes mortality but effects an immunogenic stimulation sufficient enough to cause reduced and uneven growth that is reflective of a challenging growing environment.MethodsA mixed microbial challenge was delivered as used litter containing Campylobacter jejuni and coccidial oocysts to 120 male Ross 308 broiler chicks, randomly divided into two groups: control and challenged. On day 12, 15, 18 and 22 (pre- and 3, 6 and 10 days post-addition of the used litter) the proximal jejunum was recovered from 6 replicates per group and differentially abundant proteins identified between groups and over time using 2D DiGE.ResultsThe abundance of cytoskeletal proteins of the chicken small intestinal proteome, particularly actin and actin associated proteins, increased over time in both challenged and control birds. Villin-1, an actin associated anti-apoptotic protein, was reduced in abundance in the challenged birds indicating that many of the changes in cytoskeletal protein abundance in the challenged birds were as a result of an increased rate of apoptosis. A number of heat shock proteins decreased in abundance over time in the intestine and this was more pronounced in the challenged birds.ConclusionsThe small intestinal proteome sampled from 12 to 22 days of age showed considerable developmental change, comparable to other species indicating that many of the changes in protein abundance in the small intestine are conserved among vertebrates. Identifying and distinguishing the changes in proteins abundance and molecular pathways that occur as a result of normal growth from those that occur as a result of a challenging microbial environment is important in this major food producing animal.

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An in vivo proteomic study of the interaction between Salmonella Typhimurium and porcine ileum mucosa

Cited by 32 publications

References 67 publications

The effect of microbial challenge on the intestinal proteome of broiler chickens

The effect of microbial challenge on the intestinal proteome of broiler chickens

Molecular cloning, characterization and mRNA expression of duck interleukin-17F

The effect of microbial challenge on the intestinal proteome of broiler chickens

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