Molecular cloning, characterization and mRNA expression of duck interleukin-17F

Kim, Woo Hyun; Fernandez, Cherry P.; Diaz, Joyce Anne R.; Jeong, Junhui; Kim, Suk; Lillehoj, Hyun S.; Chang, Hong; Min, Wongi

doi:10.1016/j.vetimm.2015.02.007

Cited by 7 publications

(6 citation statements)

References 36 publications

(54 reference statements)

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“…The IL-17 family cytokines induce the production of proinflammatory cytokines, chemokines, and matrix metalloproteinases (Weaver et al, 2007;Iwakura et al, 2011). Similar to the other IL-17 family members that we previously cloned, namely duIL-17A and duIL-17F (Yoo et al, 2009;Kim et al, 2015), duIL-17D treatment led to increased expression of IL-1b, IL-6, and IL-8 in primary DEF. However, duIL-17D did not induce the expression of duck TGF-b.…”

Section: Discussionsupporting

confidence: 56%

“…Our previous studies indicated that duIL-17A and duIL-17F shared 46e47% and 37e46% identities with mammalian homologues, respectively. Furthermore, duIL-17A and duIL-17F exhibited 84% and 77.5% identities with chIL-17A and chIL-17F, respectively (Min and Lillehoj, 2002;Yoo et al, 2009;Kim et al, 2012Kim et al, , 2015. Multiple sequence alignment revealed the presence of eight cysteine residues potentially involved in the formation of disulfide bonds are conserved in both avian and mammalian IL-17D.…”

Section: Discussionmentioning

confidence: 96%

“…Eight of the conserved cysteine residues were also found in IL-17D of Atlantic salmon (Kumari et al, 2009). However, duIL-17A and duIL-17F possess six of the conserved cysteine residues (Yoo et al, 2009;Kim et al, 2015), indicating that the avian IL-17D sequences contained two additional cysteines compared to the duIL-17A and IL-17F sequences. Thus, our data suggest that the newly cloned IL-17D cDNA is an avian ortholog of mammalian IL-17D.…”

Section: Discussionmentioning

confidence: 96%

“…Duck splenic lymphocytes isolated from two-week-old ducks and duck embryonic fibroblast cells (DEF) obtained from ten-dayold embryos were cultured as previously described (Jeong et al, 2015;Kim et al, 2015). Cells were collected, washed twice with PBS, and cultured in DMEM (Gibco Life Technologies, USA) supplemented with 10% fetal bovine serum (Gibco Life Technologies) and penicillin-streptomycin (10,000 unit/ml) in a 41 C incubator in 5% CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

“…A melting curve was obtained at the end of each run to verify the presence of a single amplification product without primer dimers. Relative expression levels of individual transcripts were normalized to those of b-actin using Bio-Rad CFX software as previously described (Kim et al, 2015).…”

Section: Cytokine Induction Assay and Quantitative Real-time Rt-pcr (...mentioning

confidence: 99%

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Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection

Diaz

Kim

Fernandez

et al. 2016

Developmental & Comparative Immunology

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Interleukin (IL)-17D is a proinflammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. duIL-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinflammatory cytokines such as IL-6, IL-8, and IL-1β in duck embryo fibroblast cells. Very low levels of duIL-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The duIL-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species.

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