An in vitro study of concentration specific antioxidant activity of commonly using herbs.

Abstract

We aimed to analyze the concentration dependent in vitro antioxidative or pro-oxidative activity of three commonly using herbal extracts. For this, extracts of AV (Aloe vera), AS (Annona aquamosa) and PM (Pterocarpus marsupium) were prepared. In-vitro free radicals scavenging assays such as 1, 1-Diphenyl-2-picrylhydrazyl (DPPH), Nitric oxide (NO), reducing power assay and ferrous sulphate (FeSO 4 ) induced lipid peroxidation (LPO) in rat tissue homogenates and in isolated erythrocytes were performed. Ascorbic acid was used as standard antioxidant for all chemical in vitro assays. Total polyphenols and flavonoid contents were also estimated. Concentration dependent protective effects were observed, these were also tissue specific for all tested herbs. In case of all assay systems very low and higher concentrations were found to be ineffective and less effective. Whereas, moderate drug concentrations used were observed to be significantly protective. However, pro-oxidative effects were also seen with PM and AV in LPO assay. All three test drugs were found to be antiperoxidative. However, higher concentration showed decreased efficacy indicated their harmful or less protective effects at higher doses. Significant amount of polyphenols and flavonoids present in the test drugs supported their antioxidative property. Of course more studies are required for confirmations.

Evaluation of Comparative in Vitro Free Radical Scavenging Potential of Bark Extracts of Acacia Catechu

“…The potential impact of short-term oxidative stress on the mitigation of aging and other metabolic disorders cannot be overlooked [2][3][4]. The overproduction of the same has already been known to lead to several severe impairments such as DNA mutation, DNA fragmentation, DNA damage, lipid peroxidation, protein modification, apoptosis, modification of gene expression, and membrane damage via oxidative stress [1,5,6], Thus, oxidative stress is a state of the body in which the body loses its ability to eliminate reactive free radicals and is unable to avoid harmful effects caused by them [3,7].…”

“…Moreover, seasonal changes have also been documented to affect the phytochemistry of plants [33]; hence, samples collected in different seasons of 2 successive years were also studied using the same parameter. Since in vitro studies provide a simplified model for investigation, the outcomes may be indicative of effects in in vivo systems [1,6,7]. Therefore, present in vitro research has been conducted as an initial phase of the study.…”