An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

Giri, Sarita; Takada, Ayuko; Paudel, Durga; Yoshida, Koki; Furukawa, Masayuki; Kuramitsu, Yasuhiro; Matsushita, Kazunobu; Furuichi, Yasushi

doi:10.1007/s10266-021-00630-3

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…6A). Compared with free fisetin (P < 0.001) or WPI-FIS (P < 0.01) cells was (46.9 ± 3.5)% in the G0/G1 phase and (34.9 ± 1.3)% in the G2/M phase, compared to (57.9 ± 0.5)% in the G0/G1 phase and (20.2 ± 1.5)% in the G2/M phase in the case of untreated cells, suggesting that H2O2 treatment significantly resulted in cell cycle arrest at G2/M phase in MRC5 cells (P < 0.001), which was similar to some previous reports (40,41). When the H2O2-treated MRC5 cells were treated with free fisetin, WPI-FIS or WPI-GOS-FIS, the proportions of cells in the G2/M phase were reduced to (26.3 ± 0.7)% (P < 0.001), (27.4 ± 1.5)% (P < 0.01), or (25.0 ± 0.7)% (P < 0.001), respectively, indicating that the cell cycle arrest at G2/M phase induced by H2O2 was dramatically attenuated by free or encapsulated fisetin.…”

Section: Wpi-gos-fis Inhibition Of H 2 O 2 -Induced Cellular Senescencesupporting

confidence: 91%

A food-grade and senescent cell-targeted fisetin delivery system based on whey protein isolate-galactooligosaccharides Maillard conjugate

Hou¹,

Lai²,

Song³

et al. 2024

Food Science and Human Wellness

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Cellular senescence is the results of aging and age-related diseases, and the development of anti-aging methods may improve health and extend longevity. The natural flavonol fisetin has been shown to antagonize senescence in vitro and increases longevity in vivo, but has poor water solubility and limited bioavailability. In this study, a food-grade and senescent cell-targeted delivery system for fisetin was developed based on whey protein isolate-galactooligosaccharides (WPI-GOS) Maillard conjugate, which could recognize senescence associated β-galactosidase in senescent cells. The fisetin nanoparticles possessed a high encapsulation efficiency, excellent dispersibility in water, good storage stability and well biocompatibility. Moreover, they could effectively accumulate and retain in senescent cells with excellent senescent cell-targeting efficacy, and inhibit the oxidative stress-induced cellular senescence in vitro. Thus, this novel nanoparticle system based on WPI-GOS Maillard conjugate showed promise to deliver hydrophobic bioactive ingredients like fisetin to senescent cells to improve their bioavailability and anti-senescence effect.

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Section: Wpi-gos-fis Inhibition Of H 2 O 2 -Induced Cellular Senescencesupporting

confidence: 91%

A food-grade and senescent cell-targeted fisetin delivery system based on whey protein isolate-galactooligosaccharides Maillard conjugate

Hou¹,

Lai²,

Song³

et al. 2024

Food Science and Human Wellness

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show abstract

“…Another promising bioactive flavonol with antioxidant properties is fisetin [ 270 ]. In vitro cell treatment with fisetin has shown a reduction in senescence, ROS, and apoptosis [ 270 , 271 ]. In vivo, 6-week oral administration of fisetin drastically reduced senescence, ROS, lipid peroxidation and protein oxidation in a rat model of induced aging, and lifespan extension has been reported in mice [ 235 , 272 ].…”

Section: Resultsmentioning

confidence: 99%

The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence

et al. 2022

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Cellular senescence is an irreversible state of cell cycle arrest occurring in response to stressful stimuli, such as telomere attrition, DNA damage, reactive oxygen species, and oncogenic proteins. Although beneficial and protective in several physiological processes, an excessive senescent cell burden has been involved in various pathological conditions including aging, tissue dysfunction and chronic diseases. Oxidative stress (OS) can drive senescence due to a loss of balance between pro-oxidant stimuli and antioxidant defences. Therefore, the identification and characterization of antioxidant compounds capable of preventing or counteracting the senescent phenotype is of major interest. However, despite the considerable number of studies, a comprehensive overview of the main antioxidant molecules capable of counteracting OS-induced senescence is still lacking. Here, besides a brief description of the molecular mechanisms implicated in OS-mediated aging, we review and discuss the role of enzymes, mitochondria-targeting compounds, vitamins, carotenoids, organosulfur compounds, nitrogen non-protein molecules, minerals, flavonoids, and non-flavonoids as antioxidant compounds with an anti-aging potential, therefore offering insights into innovative lifespan-extending approaches.

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“…Therefore, we used human TECs as the research object, induced aging by H 2 O 2 , and observed the effect of intervention with MSCs. H 2 O 2 is a small molecule that induce cell senescence due to oxidative stress caused by high levels of free radicals produced by the cell membrane, and increase the activity of SA-β-gal and expression of P16 and P21 [ [26] , [27] , [28] ], which were consisted with our results that the changes of TECs after treatment with 200 μmol/L H 2 O 2 for 72 h. Interestingly, when senescent TECs were cocultured with MSCs in Transwell chambers for 48 h, the activity of SA-β-gal decreased, and P16 and P21 down-regulated, chromatin was evenly distributed, and autophagosomes were observed. Moreover, under a Nanolive 4D microscope, it was found that MSCs continuously secreted vesicular substances into ageing TECs, resulting in uniform distribution of TEC chromatin, appearance of intracellular autophagosomes and smaller cell morphology.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cells reverse thymus aging by reprogramming the DNA methylation of thymic epithelial cells

Yang,

Tian,

et al. 2024

Regenerative Therapy

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An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment

Cited by 8 publications

References 34 publications

A food-grade and senescent cell-targeted fisetin delivery system based on whey protein isolate-galactooligosaccharides Maillard conjugate

A food-grade and senescent cell-targeted fisetin delivery system based on whey protein isolate-galactooligosaccharides Maillard conjugate

The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence

Mesenchymal stem cells reverse thymus aging by reprogramming the DNA methylation of thymic epithelial cells

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