An in vitro screening with emerging contaminants reveals inhibition of carboxylesterase activity in aquatic organisms

Solé, Montserrat; Sánchez-Hernández, Juan C.

doi:10.1016/j.aquatox.2015.11.001

Cited by 36 publications

(19 citation statements)

References 63 publications

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“…By contrast, CbE (pNPA) activity in fish was inhibited in the 2015 sampling. Recent in vivo and in vitro studies with PPCPs have indicated that CbE activity can be modulated by a broad range of chemicals as it participates in its metabolism and detoxification reactions and can also be inhibited in vitro by lipid regulators as it occurs in humans (Ribalta et al 2015;Solé et al 2014;Solé and Sanchez-Hernandez 2015). Many of these chemicals are found in the watercourse of the Ebre river (Lopez-Serna et al 2012;Osorio et al 2016).…”

Section: Discussionmentioning

confidence: 97%

The use of juvenile Solea solea as sentinel in the marine platform of the Ebre Delta: in vitro interaction of emerging contaminants with the liver detoxification system

Crespo

Solé

2016

Environ Sci Pollut Res

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Juveniles of Solea solea were sampled during the spring season in three consecutive years at a marine site by the mouth of the Ebre river. The aim was to assess if the extractive works from the toxic load upstream the river could be reflected on the health status of the fish living at the immediate sea. The biomarkers selected for the in vivo field study are commonly used as indicators of chemical exposures. They include activities of energy metabolism: lactate dehydrogenase (LDH) and citrate synthase (CS); neurotoxicity: cholinesterases (ChE); xenobiotic metabolism: cytochrome P450 (CYP)-dependent: EROD and BFCOD, carboxylesterase (CbE), glutathione S-transferase (GST) and uridine diphosphate glucuronyltransferase (UDPGT); and oxidative stress parameters such as catalase (CAT), glutathione reductase (GR) and glutathione peroxidase (GPX) as well as levels of lipid peroxidation (LPO). These biomarkers were mostly analysed in liver but also in gills and muscle depending on their particular tissue distribution and role. A complementary in vitro approach was also sought to see the capacity of common emerging contaminants (pharmaceuticals and personal care products; PPCPs) to interact with the liver microsomal detoxification system of the fish (EROD, BFCOD and CbE activities). The results indicated that in fish sampled in 2015 there was an enhancement in detoxification parameters (EROD, BFCOD and gill GR), muscular ChEs and gill CS, but a decrease in CbE activity and a marked oxidative stress situation (increased LPO and decreased CAT activity). Also, 4 out of the 10 PPCPs tested in vitro were able to interact with the CYP3A4 (BFCOD) enzymatic system while the lipid regulators simvastatin and fenofibrate inhibited CbE activity, as it occurs in higher vertebrates. The in vivo results support the use of a multibiomarker approach when assessing the disturbances due to chemical exposures, not only spatially but also over time, once the influence of other variables has been taken into consideration. The in vitro results highlight the importance of the CYP3A4 and CbE pathway in pharmaceutical metabolism, also in fish.

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Section: Discussionmentioning

confidence: 97%

The use of juvenile Solea solea as sentinel in the marine platform of the Ebre Delta: in vitro interaction of emerging contaminants with the liver detoxification system

Crespo

Solé

2016

Environ Sci Pollut Res

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“…The selection of BNPP and eserine was made given they are specific CE and esterase inhibitors, respectively. The choice of PPCPs and plastic additives (Table 2) was based on: i) their already reported capacity to interfere with specific mammalian CEs (references within Table 2) ii) their described CE modulation in marine species (Ribalta et al, 2015;Solé and Sanchez-Hernandez, 2015; and; iii) their environmental occurrence and bioaccumulation potential (log Kow > 3).…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%

The use of an in vitro approach to assess marine invertebrate carboxylesterase responses to chemicals of environmental concern

Solé

Freitas

Rivera‐Ingraham

2021

Environmental Toxicology and Pharmacology

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“…To the best of our knowledge, this is the first report on CbE induction by these two pharmaceutical drugs in fish although an induction by other drugs such as gemfibrozil, nonylphenol, and ethinylestradiol was formerly reported in vivo in the same fish species [31]. IBU and CBZ have also revealed to inhibit in vitro microsomal CbE activity in several marine species [28], and the lipid regulators simvastatin and fenofibrate interfere in vitro with microsomal CbE of either adults or juveniles of a related species Solea solea [28,32]. Due to the importance of this enzyme family in drug and prodrug metabolism as well as its endogenous role in lipid homeostasis, we recommended its potential use as marker of drug exposures.…”

Section: Biological Effects On Detoxification Enzymesmentioning

confidence: 71%

“…By contrast, the enzymatic responses on CYP-dependent activities and CbEs were only traceable at 15°C but not at 20°C (Table 1) [22]. An effect of rising temperature alone over these and other hepatic enzymes has been recently published for these same fish species with higher activities displayed at lower temperatures as a result of acclimation [28]. More recently, the combined effect of temperature and these same drug exposures on some of these enzymes and other metabolic and oxidative stress parameters has also been published [22].…”

Section: Biological Effects On Detoxification Enzymesmentioning

confidence: 89%

Metabolite profiling of carbamazepine and ibuprofen in Solea senegalensis bile using high-resolution mass spectrometry

et al. 2017

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The widespread occurrence of pharmaceuticals in the aquatic environment has raised concerns about potential adverse effects on exposed wildlife. Very little is currently known on exposure levels and clearance mechanisms of drugs in marine fish. Within this context, our research was focused on the identification of main metabolic reactions, generated metabolites, and caused effects after exposure of fish to carbamazepine (CBZ) and ibuprofen (IBU). To this end, juveniles of Solea senegalensis acclimated to two temperature regimes of 15 and 20°C for 60 days received a single intraperitoneal dose of these drugs. A control group was administered the vehicle (sunflower oil). Bile samples were analyzed by ultra-high-performance liquid chromatography-high-resolution mass spectrometry on a Q Exactive (Orbitrap) system, allowing to propose plausible identities for 11 metabolites of CBZ and 13 metabolites of IBU in fish bile. In case of CBZ metabolites originated from aromatic and benzylic hydroxylation, epoxidation, and ensuing O-glucuronidation, O-methylation of a catechol-like metabolite was also postulated. Ibuprofen, in turn, formed multiple hydroxyl metabolites, Oglucuronides, and (hydroxyl)-acyl glucuronides, in addition to several taurine conjugates. Enzymatic responses after drug exposures revealed a water temperature-dependent induction of microsomal carboxylesterases. The metabolite profiling in fish bile provides an important tool for pharmaceutical exposure assessment.

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An in vitro screening with emerging contaminants reveals inhibition of carboxylesterase activity in aquatic organisms

Cited by 36 publications

References 63 publications

The use of juvenile Solea solea as sentinel in the marine platform of the Ebre Delta: in vitro interaction of emerging contaminants with the liver detoxification system

The use of juvenile Solea solea as sentinel in the marine platform of the Ebre Delta: in vitro interaction of emerging contaminants with the liver detoxification system

The use of an in vitro approach to assess marine invertebrate carboxylesterase responses to chemicals of environmental concern

Metabolite profiling of carbamazepine and ibuprofen in Solea senegalensis bile using high-resolution mass spectrometry

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