An in Vitro Rainbow Trout Cell Bioassay for Aryl Hydrocarbon Receptor-Mediated Toxins

Richter, Catherine A.; Tieber, Virginia L.; Denison, Michael S.; Giesy, John P.

doi:10.1897/1551-5028(1997)016<0543:aivrtc>2.3.co;2

Cited by 13 publications

(31 citation statements)

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“…Example data set III (Fig. 3c) was generated using the RLT 2.0 bioassay, which utilizes rainbow trout hepatoma cells trans‐fected with a luciferase reporter gene under control of dioxin responsive elements to screen for Ah receptor‐active compounds [30,31]. Extracts from sediments collected from a su‐perfund site contaminated with Aroclor® 1268 (Monsanto, St. Louis, MO, USA) were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Derivation and application of relative potency estimates based on in vitro bioassay results

Villeneuve

Blankenship

Giesy

2000

Enviro Toxic and Chemistry

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254

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Abstract-Relative potency (REP) estimates are widely used to characterize and compare the potency of a wide variety of samples analyzed using in vitro bioassays. Relative potency estimates are generally calculated as a simple ratio: the EC50 of a wellcharacterized standard divided by the EC50 of a sample. Such estimates are valid only when the dose-response curves for the sample and standard are parallel and exhibit the same maximum achievable response (efficacy). These conditions are often either violated or cannot be demonstrated. As a result, there is a need to calculate and present REPs in a manner that addresses the potential uncertainties caused by violation of the assumptions of parallelism and equal efficacy. Multiple point estimates, over the range of responses from EC20 to EC80, can be used to derive relative potency ranges (REP 20-80 range). The width of a REP range is directly proportional to the degree of deviation from parallelism between sample and standard dose-response curves. Thus, REP 20-80 ranges both test the assumption of parallelism and characterize the amount of uncertainty in an REP estimate resulting from deviation from parallelism. Although uncertainties due to unequal efficacy cannot be easily characterized mathematically, a systematic method for evaluating sample efficacy has been developed into a framework to guide the derivation and application of REP estimates based on in vitro bioassay results. Use of the systematic framework and REP 20-80 ranges was illustrated using three sample data sets. It is hoped that the framework and discussion presented will facilitate the use of bioassay-derived REP estimates to characterize samples of both known and unknown composition without ignoring the assumptions underlying REP estimation.

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Section: Methodsmentioning

confidence: 99%

Derivation and application of relative potency estimates based on in vitro bioassay results

Villeneuve

Blankenship

Giesy

2000

Enviro Toxic and Chemistry

Self Cite

254

220

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“…MVLN cells are MCF‐7 human breast carcinoma cells stably transfected with a luciferase reporter gene under control of estrogen response elements of the Xenopus vitellogenin A2 gene [23]. H4IIE‐luc cells and RLT 2.0 cells are rat and rainbow trout hepatoma cells, respectively, which were stably transfected with a luciferase reporter gene under control of dioxin‐responsive enhancers (DREs) [20,24]. All cells were cultured in 100‐mm disposable petri plates (Corning, Corning, NY, USA) and were incubated in a humidified 95:5 air:CO 2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

Alkylphenols, polycyclic aromatic hydrocarbons, and organochlorines in sediment from Lake Shihwa, Korea: Instrumental and bioanalytical characterization

Khim

Villeneuve

Kannan

et al. 1999

Enviro Toxic and Chemistry

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Lake Shihwa is an artificial lake, located on the west coast of Korea, that has experienced environmental deterioration since 1994, when it was formed by construction of a sea dike. This study used instrumental analysis and in vitro bioassays to characterize organic contaminants in sediment collected from 11 stations on Lake Shihwa. Alkylphenol (AP) concentrations in Lake Shihwa sediment ranged from 20.2 to 1,820 ng/g nonylphenol and from 4.69 to 50.5 ng/g octylphenol, on a dry weight basis. Maximum concentrations of polycyclic aromatic hydrocarbons (PAHs), organochlorine pesticides, and polychlorinated biphenyls (PCBs) were 30.8, 2.26, and 12.3 ng/g (dry weight), respectively. Significant estrogenic activity was associated with fractions containing APs. Mass-balance analysis suggested that reported concentrations of APs account for less than 20% of the estrogenic activity observed. No significant dioxinlike activity was associated with fractions containing classic aryl hydrocarbon receptor agonists, such as PCBs, but the mid-polarity fractions containing PAHs and most polar fractions yielded significant dioxinlike activity. Overall, most of the in vitro bioassay responses appear to have been caused by unidentified and/or undetectable compounds associated with Lake Shihwa sediment. Keywords-AlkylphenolsSediment In vitro bioassay Polycyclic aromatic hydrocarbons Polychlorinated biphenyls Estrogen-and dioxinlike potency of sediment contaminants

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“…A similar system was generated previously in carp cells (Muñoz et al, 2005;Rocha et al, 2004). Single recombinant cell lines producing luciferase had been established in the past as a tool to study specific sequences, to monitor toxicity of samples to fish cells (Richter et al, 1997) or to study signalling mechanisms but the transgene was always a reporter gene luciferase linked to a regulatory sequence or encoding an endogenous gene (Collet et al, 2004;Collet and Secombes, 2005;Martin et al, 2008). Previous attempts to force fish cell to produce ISAV proteins using plasmids with strong promoters such as CMV failed to produce any recombinant cells probably because of the toxicity of the viral proteins.…”

mentioning

confidence: 99%

Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system

Collet

Lester

2011

Journal of Biotechnology

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An in Vitro Rainbow Trout Cell Bioassay for Aryl Hydrocarbon Receptor-Mediated Toxins

Cited by 13 publications

References 0 publications

Derivation and application of relative potency estimates based on in vitro bioassay results

Derivation and application of relative potency estimates based on in vitro bioassay results

Alkylphenols, polycyclic aromatic hydrocarbons, and organochlorines in sediment from Lake Shihwa, Korea: Instrumental and bioanalytical characterization

Establishment of an Atlantic salmon kidney cell line with an inducible gene expression system

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