An in vitro evaluation of the Ca/P ratio for the cytocompatibility of nano-to-micron particulate calcium phosphates for bone regeneration

Liu, Huinan; Yazıcı, Hilal; Ergun, Celaletdin; Webster, Thomas J.; Bermek, Hakan

doi:10.1016/j.actbio.2008.02.025

Cited by 223 publications

(139 citation statements)

References 22 publications

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“…10 Relative cell viability of USC exposed to different concentrations of calcium phosphate nanoparticles at different Ca/P ratios. Cells that were not exposed to nanoparticles were used as control (n = 5) to phosphate molar ratio and changes in structural properties such as crystallinity, porosity and particle size result in differences in behaviors of particles (Liu et al 2008). In this study, properties of nanoparticles of calcium phosphates at different Ca/P molar ratios were studied.…”

Section: Discussionmentioning

confidence: 99%

“…Amorphous calcium phosphates (ACPs) are one of the widely used biomaterials for tissue engineering applications as ionic cement, coating of prosthesis, and mineral organic composites to benefit from their physicochemical behavior (Brunner et al 2007a;Liu et al 2008;Combes and Rey 2010;He et al 2013). Reactivity of calcium phosphates (dissolution from the surface of material and re-precipitation as apatite) in biological environments or stimulated body fluids is called bioactivity of these biomaterials.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

et al. 2015

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The present study evaluates the synthesis of biocompatible osteoconductive and osteoinductive nano calcium phosphate (CaP) particles by industrially applied, aerosol-derived flame spray pyrolysis method for biomedical field. Calcium phosphate nanoparticles were produced in a range of calciumto-phosphorus ratio, (1.20-2.19) in order to analyze the morphology and crystallinity changes, and to test the bioactivity of particles. The characterization results confirmed that nanometer-sized, spherical calcium phosphate particles were produced. The average primary particle size was determined as 23 nm by counting more than 500 particles in TEM pictures. XRD patterns, HRTEM, SAED, and SEM analyses revealed the amorphous nature of the asprepared nano calcium phosphate particles at low Ca/P ratios. Increases in the specific surface area and crystallinity were observed with the increasing Ca/P ratio. TGA-DTA analysis showed that the thermally stable crystal phases formed after 700°C. Cell culture studies were conducted with urine-derived stem cells that possess the characteristics of mesenchymal stem cells. Synthesized amorphous nanoparticles did not have cytotoxic effect at 5-50 lg/ml concentration range. Cells treated with the as-prepared nanoparticles had higher alkaline phosphatase (ALP) enzyme activity than control cells, indicating osteogenic differentiation of cells. A slight decrease in ALP activity of cells treated with two highest Ca:P ratios at 50 lg/ml concentration was observed at day 7. The findings suggest that calcium phosphate nanoparticles Guest Editors: Mustafa Culha, Rawil F. Fakhrullin, Ratnesh Lal This article is part of the topical collection on Nanobiotechnology Electronic supplementary material The online version of this article

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

et al. 2015

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“…The biological activity of calcium phosphate ceramic materials is thought to be influenced by various physicochemical factors, such as chemical composition, crystal size, surface nanostructure and more [32][33][34] . The effect of calcium and Pi extracellular ion concentration has also been reported to affect osteoblast differentiation 35,36) .…”

Section: Discussionmentioning

confidence: 99%

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

et al. 2014

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The present study investigated the role of mitogen-activated protein kinase (MAPK) signaling in osteoblastic differentiation of stromal ST-2 cells induced by synthetic octacalcium phosphate (OCP) incubation. Since our previous studies revealed that OCP consumes calcium ions in media during conversion to hydroxyapatite, the effect of the ions on ST-2 cell differentiation with or without OCP crystals was analyzed. The effect of presence or absence of MAPK inhibitors was also analyzed. OCP increased alkaline phosphatase (ALP) activity and the mRNA expression of differentiation markers via the p38 signaling pathway. The PD98059 MAPK inhibitor increased ALP activity and differentiation marker genes in cells cultured in OCP-coated wells. Reduction of calcium ions in the medium by EGTA increased the ALP activity without OCP in the presence of phosphate ion concentrations up to 7.5 mM. OCP may enhance osteoblastic differentiation through the p38 signaling pathway via the reduction of calcium ions induced by its physicochemical property.

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“…. Calcium phosphate based bioceramics have been widely used for orthopedic applications owing to their chemical similarity to natural bone 12) .…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of Early Bone Formation with PRF, Bio-Oss and Osteoid Hydroxyapatite after Tooth Extraction in Rabbits

Pei

Nie

Yang

et al. 2015

J. Hard Tissue Biology.

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The objective of this study was to compare the ability of platelet-rich fibrin (PRF), Bio-Oss and osteoid hydroxyapatite (OHA) in early bone formation by filling tooth extraction sockets in rabbits. 48 rabbits were randomly divided into 4 groups: group A (PRF), group B (Bio-Oss), group C (OHA), and group D (control). One of the mandibular central incisors was extracted and instantly filled with graft materials. General, radiological and histological observations were evaluated 1, 2, 3, 4, 6, and 8 weeks later. In the first 4 weeks, the quantity of new bone in the PRF group alveolar defects was the best, and the bone mineral density was significantly higher. At the 6 th and 8 th weeks, the speed of new bone formation in the Bio-Oss group and the OHA group was better than that in the PRF group. Compared with the Bio-Oss group, the OHA group had osteoblasts which were more active and a slightly larger number of bone trabeculae. In addition, the control group was worse than other groups in bone-formation. These results indicate that PRF, OHA and Bio-Oss all can promote osteogenesis in tooth extraction sockets, but the effect of PRF is remarkable at the early time of bone formation. At the later stage, both Bio-Oss and OHA can repair bone defects and guide bone regeneration, and the potential bone formation ability of OHA is better.

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An in vitro evaluation of the Ca/P ratio for the cytocompatibility of nano-to-micron particulate calcium phosphates for bone regeneration

Cited by 223 publications

References 22 publications

Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells

Osteoblastic differentiation of stromal ST-2 cells from octacalcium phosphate exposure via p38 signaling pathway

A Comparative Study of Early Bone Formation with PRF, Bio-Oss and Osteoid Hydroxyapatite after Tooth Extraction in Rabbits

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