An In Vitro Evaluation of the Biological Effects of Carbon Nanotube-Coated Dental Zirconia

Abstract

The purpose of this study is to evaluate functionalized multiwalled carbon nanotubes (fMWCNTs) as a potential coating material for dental zirconia from a biological perspective: its effect on cell proliferation, viability, morphology, and the attachment of an osteoblast-like cell. Osteoblast-like (Saos-2) cells were seeded on uncoated and fMWCNT-coated zirconia discs and in culture dishes that served as controls. The seeding density was 104 cells/cm2, and the cells were cultured for 6 days. Cell viability, proliferation and attachment of the Saos-2 cells were studied. The results showed that Saos-2 cells were well attached to both the uncoated and the fMWCNT-coated zirconia discs. Cell viability and proliferation on the fMWCNT-coated zirconia discs were almost the same as for the control discs. Better cell attachment was seen on the fMWCNT-coated than on the uncoated zirconia discs. In conclusion, fMWCNTs seem to be a promising coating material for zirconia-based ceramic surfaces to increase the roughness and thereby enhance the osseointegration of zirconia implants.

Zirconia surface modifications for implant dentistry

“…The a-C:H:N coated Y-TZP surfaces revealed a low adhesion of bacteria on the surface compared to uncoated surfaces [83]. Other studies have also coated zirconia with functionalized multiwalled carbon nano-tubes increasing the roughness, wettability, and cell adhesion of this material favoring zirconia´s osseintegration properties [90,91].…”

Nanoparticles used in dentistry: A review

“…Nanotubes can induce inflammatory and fibrotic reactions under some conditions by crossing membrane barriers. [98][99][100][101][102] Graphene Teeth coating, suitable for implantation, biofilm reduction Cost effectiveness, fracture resistant, low density form a uniform crystal lattice, treat bacterial biofilm…”

The Significance and Insignificance of Carbon Nanotube-Induced Inflammation

“…Applications of CNTs, which may allow ingestion, are steadily increasing, and can include their implementation as adsorbents in water purification systems [141], or as antimicrobial agents or stabilizing materials in food packaging [142]. Recently, Prajapati et al [143] investigated the use of CNTs as carriers for orally administered anti-parasite treatment in rats, with possible application in humans, and Kou et al [144] identified functionalized MWCNTs as a promising coating material of dental implants for better osseointegration [144]. There have been observations that residual hydrophobic compounds, which are often adsorbed to CNTs during their synthesis, were released more rapidly in the presence of gastric enzymes and increased salt concentration in simulated GI fluids.…”