An In Vitro Assay of Disinfectants on the Viability of Heterakis gallinarum Eggs

Cupo, Katherine Lynn; Beckstead, Robert B.

doi:10.1637/11952-081418-resnote.1

Cited by 5 publications

(2 citation statements)

References 8 publications

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“…Meanwhile, there is a sufficient number of standardized methods, in particular, using cell cultures of different organ natures, which allow obtaining adequate information for assessing the impact of xenobiotics on cell metabolism [4], [5], [6]. The variety of test systems is extraordinary, and more than 170 commercial organizations offer in vitro toxicity testing services covering mechanisms such as genotoxicity, hepatotoxicity, cardiotoxicity, and immunotoxicity [7], [8].…”

Section: Introductionmentioning

confidence: 99%

The study of the cytotoxic effect of disinfectants

Iakubchak

Adamenko²,

Таран³

et al. 2023

Potr. S. J. F. Sci.

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The toxicity of individual disinfectants has been studied in vitro using human cell cultures (HT-29 (epithelial-like cells of colon adenocarcinoma), HEK 293 (human embryonic kidney cells)) to create a model for assessing the toxicity of residual amounts of disinfectants that can enter milk for a person. Standard tests have been used to assess cell viability and amount: methyl tetrazolium (MTT) test, neutral red cell staining (NRP), and sulforhodamine B (SRB) test. Disinfectants have a dose- and time-dependent cytotoxic effect on human cell cultures. IC50avg (concentration of the drug that suppresses a certain cell function by 50%) of disinfectants based on the effect on cell cultures (average value) is Biodez – 117.29 ±14 μl/l, Blanidas – 389.25 ±20.83 μl/l, Virkon-S – 343.04 ±28.04 μl/l, Neochlor – 473.82 ±30.16 μl/l, Phan – 56.71 ±7.05 μl/l, Chlorination – 343.28 ±27.26 μl/l, Chlorinated lime – 117.35 ±9.44 μl/l. Mean toxic doses for cell cultures are lower than the mean lethal dose (based on literature data) for rats and mice by gastric administration. The novelty is that determining the cytotoxicity of disinfectants in vitro using human cell cultures can significantly reduce the number of animals for establishing LD50 during the registration procedure of new agents, making it possible to make preliminary conclusions about the toxicity of substances at the stage of chemical screening, preliminary hygienic regulation, identify target organs of toxic influence.

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Section: Introductionmentioning

confidence: 99%

The study of the cytotoxic effect of disinfectants

Iakubchak

Adamenko²,

Таран³

et al. 2023

Potr. S. J. F. Sci.

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“…Therefore, in recent years, a number of studies have been conducted to determine the disinfectant properties of preparations against individual helminthiasis in relation to the embryonic stages of its development. In particular, nine disinfectants and chemicals were tested on Heterakis gallinarum eggs, among which only NaOCl had pronounced disinfectant properties (Cupo & Beckstead, 2019). When studying the effect of 6 commercial disinfectants on larval development in Toxascaris leonina eggs, scientists found that only Dettol ® and Virkon ® caused 100% larval death (P < 0.05) (El-Dakhly et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The influence of chemicals on the development and viability of Trichuris vulpis nematode eggs isolated from infested dogs

Yevstafieva,

Dolhin,

Melnychuk

et al. 2023

Regul. Mech. Biosyst.

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Preventive measures against invasive diseases should be carried out taking into account the peculiarities of the life cycle of helminths. This is especially relevant for geohelmintiases, where the infection is mainly transmitted via environmental objects contaminated with the pathogen at the exogenous stages of the parasite’s development. It has been proven that among the causative agents of nematodoses of the gastrointestinal tract of animals, the eggs of geohelminths of the genus Trichuris are the most resistant to the action of disinfectants. The aim of the research was to establish in laboratory conditions the ovicidal effect of chemicals on the development and viability of Trichuris vulpis nematode eggs isolated from infested dogs. Two disinfectants were tested in vitro, Arquades-plus (dialkyl dimethyl ammonium chloride, dodecyl dimethyl ammonium chloride, tetrasodium salt) and Brovadez-plus (alkyl dimethyl benzyl ammonium chloride, dodecyl dimethyl ammonium chloride). Arquades-plus disinfectant showed a high level of ovicidal efficiency (94.0%) only at the maximum concentration and exposure (2.0%, 60 min), and 4.7% of nematode eggs in the test culture remained viable. Brovadez-plus disinfectant was less effective and did not provide a high level of ovicidal efficiency against Trichuris eggs in the studied concentrations and exposures. Satisfactory and unsatisfactory levels of its ovicidal effect (63.1–77.1% and 2.5–58.9%) were established when using the product in concentrations of 2.0% and 0.5–1.5%, respectively, for exposures of 10–60 min. The disinfestation activity of the preparations was characterized by metric changes in experimental test cultures compared to the control test culture. Under the influence of Arquades-plus at all concentrations, the length of the eggs was greater (by 0.4–2.0%), and the width was smaller (by 0.7–4.9%). Under the influence of Brovadez-plus in concentrations of 1.0–2.0%, the length of the eggs was greater (by 0.5–0.9%), the width was smaller (by 0.3–4.1%). The results of the conducted studies allow us to recommend the Arquades-plus disinfectant in a 2.0% concentration (exposure of 60 minutes) for effective control and prevention of infection of dogs with the causative agent of trichurosis.

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