An in situ forming collagen–PEG hydrogel for tissue regeneration

Sargeant, Timothy D.; Desai, Arpan P.; Banerjee, Saumya; Agawu, Atu; Stopek, Joshua

doi:10.1016/j.actbio.2011.07.028

Cited by 147 publications

(125 citation statements)

References 66 publications

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“…As cartilage-bone unit has been gaining more and more attentions [5, 11], the notion of cartilage repair should turn to the more comprehensive view of osteochondral repair [8]. Increasing evidences showed that the subchondral bone may play a significant role in the onset, repair or progression of cartilage damage [5, 13, 21–26]; thus the role of subchondral bone should be considered in order to achieve functional restoration during osteochondral repair.…”

Section: Discussionmentioning

confidence: 99%

Cartilage Repair and Subchondral Bone Migration Using 3D Printing Osteochondral Composites: A One-Year-Period Study in Rabbit Trochlea

Zhang

Lian

et al. 2014

BioMed Research International

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Increasing evidences show that subchondral bone may play a significant role in the repair or progression of cartilage damage in situ. However, the exact change of subchondral bone during osteochondral repair is still poorly understood. In this paper, biphasic osteochondral composite scaffolds were fabricated by 3D printing technology using PEG hydrogel and β-TCP ceramic and then implanted in rabbit trochlea within a critical size defect model. Animals were euthanized at 1, 2, 4, 8, 16, 24, and 52 weeks after implantation. Histological results showed that hyaline-like cartilage formed along with white smooth surface and invisible margin at 24 weeks postoperatively, typical tidemark formation at 52 weeks. The repaired subchondral bone formed from 16 to 52 weeks in a “flow like” manner from surrounding bone to the defect center gradually. Statistical analysis illustrated that both subchondral bone volume and migration area percentage were highly correlated with the gross appearance Wayne score of repaired cartilage. Therefore, subchondral bone migration is related to cartilage repair for critical size osteochondral defects. Furthermore, the subchondral bone remodeling proceeds in a “flow like” manner and repaired cartilage with tidemark implies that the biphasic PEG/β-TCP composites fabricated by 3D printing provides a feasible strategy for osteochondral tissue engineering application.

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Section: Discussionmentioning

confidence: 99%

Cartilage Repair and Subchondral Bone Migration Using 3D Printing Osteochondral Composites: A One-Year-Period Study in Rabbit Trochlea

Zhang

Lian

et al. 2014

BioMed Research International

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“…A significant problem with using collagen as the main component of scaffolds for skin substitutes is it has relatively poor mechanical properties[33]. However, these properties can be improved with various cross-linking methods, typically cross-linking collagen with glycosaminoglycans[34–36], but also with hyaluronic acid[37], fibrin[38], chitosan[30,39,40], gelatin[41,42], elastin[43], pullulan[44–46], alginate[47,48], laminin[49], poly(L-lactic acid) (PLLA)[50], poly(glycolide-co-L-lactide) (PLGA)[51,52], poly(ethylene glycol) (PEG)[53], and polycaprolactone (PCL)[54]. …”

Section: Components Of a Skin Substitutementioning

confidence: 99%

Methodologies in creating skin substitutes

Nicholas

Jeschke

Amini-Nik

2016

Cell. Mol. Life Sci.

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The creation of skin substitutes has significantly decreased morbidity and mortality of skin wounds. Although there are still a number of disadvantages of currently available skin substitutes, there has been a significant decline in research advances over the past several years in improving these skin substitutes. Clinically most skin substitutes used are acellular and do not use growth factors to assist wound healing, key areas of potential in this field of research. This article discusses the five necessary attributes of an ideal skin substitute. It comprehensively discusses the three major basic components of currently available skin substitutes: scaffold materials, growth factors, and cells, comparing and contrasting what has been used so far. It then examines a variety of techniques in how to incorporate these basic components together to act as a guide for further research in the field to create cellular skin substitutes with clinically better results.

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“…13, 14, 28 More recently, hybrid hydrogels, composed of recombinant elastomeric protein and PEG, have yielded materials in which encapsulated cells spread and adopt a spindle-like morphology. 17, 29 In addition, protein-PEG hydrogels comprising other proteins such as collagen 30–32 or fibrinogen, 30, 33–36 have been shown to improve biocompatibility and cell-matrix interaction. 35 …”

Section: Introductionmentioning

confidence: 99%

Resilin-PEG Hybrid Hydrogels Yield Degradable Elastomeric Scaffolds with Heterogeneous Microstructure

McGann¹,

Akins

Kiick

2015

Biomacromolecules

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Hydrogels derived from resilin-like polypeptides (RLPs) have shown outstanding mechanical resilience and cytocompatibility, and expanding the versatility of RLP-based materials via conjugation with other polypeptides and polymers would offer great promise in the design of a range of materials. Here, we present an investigation of the biochemical and mechanical properties of hybrid hydrogels composed of a recombinant RLP and a multi-arm PEG macromer. These hybrid hydrogels can be rapidly cross-linked through a Michael-type addition reaction between the thiols of cysteine residues on the RLP and vinyl sulfone groups on the multi-arm PEG. Oscillatory rheology and tensile testing confirmed the formation of elastomeric hydrogels with mechanical resilience comparable to aortic elastin; hydrogel stiffness was easily modulated through the cross-linking ratio. Macromolecular phase separation of the RLP-PEG hydrogels offers the unique advantage of imparting a heterogeneous microstructure, which can be used to localize cells, through simple mixing and crosslinking. Assessment of degradation of the RLP by matrix metalloproteinases (MMPs) illustrated the specific proteolysis of the polypeptide in both its soluble form and when cross-linked into hydrogels. Finally, the successful encapsulation and viable three-dimensional culture of human mesenchymal stem cells (hMSCs) demonstrated the cytocompatibility of the RLP-PEG gels. Overall, the cytocompatibility, elastomeric mechanical properties, micro-heterogeneity, and degradability of the RLP-PEG hybrid hydrogels offer a suite of promising properties for the development of cell-instructive, structured tissue engineering scaffolds.

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An in situ forming collagen–PEG hydrogel for tissue regeneration

Cited by 147 publications

References 66 publications

Cartilage Repair and Subchondral Bone Migration Using 3D Printing Osteochondral Composites: A One-Year-Period Study in Rabbit Trochlea

Cartilage Repair and Subchondral Bone Migration Using 3D Printing Osteochondral Composites: A One-Year-Period Study in Rabbit Trochlea

Methodologies in creating skin substitutes

Resilin-PEG Hybrid Hydrogels Yield Degradable Elastomeric Scaffolds with Heterogeneous Microstructure

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