An in-depth comparison of the male pediatric and adult urinary proteomes

Abstract

In this study, we performed an in-depth characterization of the male pediatric infant urinary proteome by parallel proteomic analysis of normal healthy adult (n =6) and infant (n =6) males and comparison to available published data. A total of 1584 protein groups were identified. Of these, 708 proteins were identified in samples from both cohorts. Although present in both cohorts, 136 of these common proteins were significantly enriched in urine from adults and 94 proteins were significantly enriched in urine from infants. Using Gene Ontology, we found that the infant-enriched or specific subproteome (743 proteins) had an overrepresentation of proteins that are involved in translation and transcription, cellular growth and metabolic processes. In contrast, the adult enriched or specific subproteome (364 proteins) showed an overexpression of proteins involved in immune response and cell adhesion. This study demonstrates that the non-diseased male urinary proteome is quantitatively affected by age, has age-specific subproteomes, and identifies a common subproteome with no age-dependent abundance variations. These findings highlight the importance of age-matching in urinary proteomics.

Investigation of urine proteome of preterm newborns with respiratory pathologies

“…On average, 242±48 proteins were identified in each sample. The dynamic range of the infant urine proteome covers 6 orders of magnitude, for adults -5 orders for healthy pregnant women and 6 orders for healthy men, what is comparable with other data published by other groups [8,[14][15][16][17]. In particular C.H.…”

“…Proteins associated with the endosome were found in newborns only. The distribution of urine proteins from newborns in these GO terms was found to be in a good agreement with previous studies [7][8] leading to the conclusion about the high level of apoptotic processes in the bodies of preterm infants, associated, with high level of oxidative stress and rapid development of all body systems.…”

“…The only systemic study of a healthy infant urine proteome (6 newborns) was performed by J.W. Froehlich and colleagues (2014) in comparison with an adult group (6 healthy men) [8]. The authors identified a total of 1584 proteins and 94 proteins were significantly enriched in neonate urine, representing the enhancement of metabolic processes, cellular growth, translation and transcription.…”

“…Additional sample fractionation allowed Marimuthu and colleagues to add 671 proteins to this catalog [15]. Different strategies are applied for shotgun urinary proteomics, including semi-quantitative label-free HPLC-MS/MS, 2D-PAGE-MS/MS or CE-MS/MS [8][9][10][11][12][13]18], quantitative SRM/MRM HPLC-MS/MS [15][16][17].…”

“…Non-invasive MS based methods for monitoring the status of children with ELBW and VLBW are based on the analysis of easily accessible biomaterials such as buccal scrapings [5], exhaled breath condensate [6], and urine [7][8][9][10]. Biomarkers in the urine samples of infants are widely used for predicting such pathologies as obstructive nephropathy [11,12], necrotizing enterocolitis [13], and for monitoring maturation and development of the urinary system [7].…”