An improvement in acute wound healing in mice by the combined application of photobiomodulation and curcumin-loaded iron particles

Moradi, Ali; Kheirollahkhani, Yashar; Fatahi, Payam; Abdollahifar, Mohammad‐Amin; Amini, Abdollah; Naserzadeh, Parvaneh; Ashtari, Khadijah; Ghoreishi, Seyed Kamran; Chien, Sufan; Rezaei, Fatemehalsadat; Fridoni, Mohammadjavad; Bagheri, Mohammad; Taheri, Salman; Bayat, Mohammad

doi:10.1007/s10103-018-2664-9

Cited by 33 publications

(33 citation statements)

References 34 publications

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“…[25][26][27][28] The latest experiments in our laboratory have revealed that joint administration of PBM and curcumin meaningfully reduced colony forming units in a type one diabetes mellitus (T1DM) animal model, 29 and increased the wound contraction ratio and wound toughness in a normal mouse. 30 Therefore, we propose that additional investigations should be conducted that might lead to the use of PBM + curcumin to treat DFU. The mechanisms that underlie the optimistic influence of PBM on skin injuries should be additionally analyzed by investigating the changes to the wound bed's histology and expression of growth factors that occur subsequent to PBM.…”

Section: Introductionmentioning

confidence: 99%

Stereological and gene expression examinations on the combined effects of photobiomodulation and curcumin on wound healing in type one diabetic rats

Amini

Soleimani

Abdollhifar

et al. 2019

J of Cellular Biochemistry

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We examined the effects of photobiomodulation (PBM) independently and combined with curcumin on stereological parameters and basic fibroblast growth factor (bFGF), hypoxia‐inducible factor‐1α (HIF‐1α), and stromal cell‐derived factor‐1α (SDF‐1α) gene expressions in an excisional wound model of rats with type one diabetes mellitus (T1DM). T1DM was induced by an injection of streptozotocin (STZ) in each of the 90 male Wistar rats. One round excision was generated in the skin on the back of each of the 108 rats. The rats were divided into six groups (n = 18 per group): control (diabetic), untreated group; vehicle (diabetic) group, which received sesame oil; PBM (diabetic) group; curcumin (diabetic) group; PBM + curcumin (diabetic) group; and a healthy control group. On days 4, 7, and 15, we conducted both stereological and quantitative real‐time PCR (qRT‐PCR) analyses. The PBM and PBM + curcumin groups had significantly better inflammatory response modulation in terms of macrophages (P < .01), neutrophils (P < .001), and increased fibroblast values compared with the other groups at day 4 (P < .001), day 7 (P < .01), and day 15 (P < .001). PBM treatment resulted in increased bFGF gene expression on days 4 (P < .001) and 7 (P < .001), and SDF‐1α gene expression on day 4 (P < .001). The curcumin group had increased bFGF (P < .001) expression on day 4. Both the PBM and PBM + curcumin groups significantly increased wound healing by modulation of the inflammatory response, and increased fibroblast values and angiogenesis. The PBM group increased bFGF and SDF‐1α according to stereological and gene expression analyses compared with the other groups. The PBM and PBM + curcumin groups significantly increased the skin injury repair process to more rapidly reach the proliferation phase of the wound healing in T1DM rats.

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Section: Introductionmentioning

confidence: 99%

Stereological and gene expression examinations on the combined effects of photobiomodulation and curcumin on wound healing in type one diabetic rats

Amini

Soleimani

Abdollhifar

et al. 2019

J of Cellular Biochemistry

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“…The skin injury areas were calculated by image J-NIH (USA). Skin injury area measurement was used to show and measure wound closure [21].…”

Section: Wound Area Measurement Methodsmentioning

confidence: 99%

An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine

et al. 2019

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In this probe, at first we examined the best route and dosage of arginine administration on wound healing in an excisional wound model in rats. Next, we intend to assess the impact of photobiomodulation (PBM) and arginine, individually and together, on the wound healing. In the pilot study, an excisional wound was made in each of 24 rats. There were 4 groups. Group 1 was the control group. In groups 2 and 3, wounds were topically treated with arginine ointments (ARG.) 2% and 5%, respectively. In group 4, arginine was injected (ARG. INJ.,i.p.). In the main phase, in 24 new rats, an excisional wound was made. There were 4 groups: group 5 served as the control. Wounds in group 6 were topically treated with ARG 2%. Wounds in group 7 were subjected to PBM. Wounds in group 8 were treated with PBM+ARG. 2%. On day 15, wound area measurement, wound strength, and stereological examination were performed. In the pilot study, we found that the ARG 2% ointment significantly decreased wound area than ARG. 5%, ARG. INJ. and control groups, and significantly increased wound strength compared to the control and ARG.5% groups. In the main phase, a significant decrease of wound area in all treatment regimens was induced. PBM + ARG. 2% and PBM treatment regimens significantly improved wound strength and almost all stereological parameters, compared to the control and ARG. 2% groups. PBM + ARG. 2% induced anti-inflammatory and angiogenic activities, and hastened the wound healing process in an excisional wound model in rats.

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“…The wound area (mm 2 ) was computed and compared to day 0 using Image J-NIH (USA). The times of complete wound closure were recorded and reported as the wound closure rate 30 .…”

Section: Ads Transplantationmentioning

confidence: 99%

“…The authors showed that photobiomodulation (890 nm, 80 Hz, 0.324 J/cm 2 ) meaningfully hastened the repair of injured skin at the maturation step and significantly decreased colony forming units (CFUs) of bacteria in a non-genetic model of DM2 in rats 26 .More than 100 recognized factors are involved in the insufficiency of wound healing in patients with DM2 27 . The use of a mixture of therapeutic agents and biological biostimulators could assist with treatment of non-healing wounds and show a synergistic effect to improve the success of healing in different wound models [28][29][30] .In the present study we focused on the effects of photobiomodulation and ADS, alone and combined, on the maturation step of an ischemic, delayed healing, infected wound model in DM2 rats. The combined application of photobiomodulation and ADS could hasten the healing process and provide a treatment for severe cases of DFUs in patients with DM2.…”

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Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats

Moradi

Zare

Mostafavinia

et al. 2020

Sci Rep

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in this study, we sought to investigate the impact of photobiomodulation and adipose-derived stem cells (ADS), alone and in combination, on the maturation step of wound healing in an ischemic infected delayed healing wound model in rats with type 2 diabetes mellitus (DM2). We randomly divided 24 adult male rats into 4 groups (n = 6 per group). DM2 plus an ischemic delayed healing wound were induced in all rats. the wounds were infected with methicillin-resistant Staphylococcus aureus. Group 1 was the control (placebo) group. Group 2 received only photobiomodulation (890 nm, 80 Hz, 0.324 J/cm 2 , and 0.001 W/cm 2 ). Group 3 received only the allograft ADS. Group 4 received allograft ADS followed by photobiomodulation. On days 0, 4, 8, 12, and 16, we performed microbiological examination (colony forming units, [CFU]), wound area measurement, wound closure rate, wound strength, and histological and stereological examinations. The results indicated that at day 16, there was significantly decreased cfU (Analysis of variance, p = 0.001) in the photobiomodulation + ADS (0.0 ± 0.0), ADS (1350 ± 212), and photobiomodulation (0.0 ± 0.0) groups compared with the control group (27250 ± 1284). There was significantly decreased wound area (Analysis of variance, p = 0.000) in the photobiomodulation + ADS (7.4 ± 1.4 mm 2 ), ADS (11 ± 2.2 mm 2 ), and photobiomodulation (11.4 ± 1.4 mm 2 ) groups compared with the control group (25.2 ± 1.7). There was a significantly increased tensiometeric property (stress maximal load, Analysis of variance, p = 0.000) in the photobiomodulation + ADS (0.99 ± 0.06 N/ cm 2 ), ADS (0.51 ± 0.12 N/cm 2 ), and photobiomodulation (0.35 ± 0.15 N/cm 2 ) groups compared with the control group (0.18 ± 0.04). There was a significantly modulated inflammatory response in (Analysis of variance, p = 0.049) in the photobiomodulation + ADS (337 ± 96), ADS (1175 ± 640), and photobiomodulation (69 ± 54) treatments compared to control group (7321 ± 4099). photobiomodulation + ADS gave significantly better improvements in CFU, wound area, and wound strength compared to photobiomodulation or ADS alone. Photobiomodulation, ADS, and their combination significantly hastened healing in ischemic methicillin-resistant Staphylococcus aureus infected delayed healing wounds in rats with DM2. Combined application of photobiomodulation plus ADS demonstrated an additive effect.Type 2 diabetes mellitus (DM2) is an important worldwide health problem with a high prevalence in the United States 1 . It is anticipated that the number of adults who suffer from DM2 will increase 2 . More than 100 million adults in the United States currently suffer from DM or pre-DM. It has been reported that 90-95% of patients with DM in the United States have DM2 and only 5% are recognized as having type 1 DM (DM1) 2 .The repair of injured skin (skin wound healing) is a physiological reaction towards interruption of skin integrity that needs coordinated regulation of different cells and growth factors. This complex course of healing is susceptible to imb...

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An improvement in acute wound healing in mice by the combined application of photobiomodulation and curcumin-loaded iron particles

Cited by 33 publications

References 34 publications

Stereological and gene expression examinations on the combined effects of photobiomodulation and curcumin on wound healing in type one diabetic rats

Stereological and gene expression examinations on the combined effects of photobiomodulation and curcumin on wound healing in type one diabetic rats

An improvement in acute wound healing in rats by the synergistic effect of photobiomodulation and arginine

Photobiomodulation plus Adipose-derived Stem Cells Improve Healing of Ischemic Infected Wounds in Type 2 Diabetic Rats

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