An Improved UPLC Method for Rapid Analysis of Levofloxacin in Human Plasma

Park, Daejin; Phapale, Prasad; Jang, In‐Jin; Song, Chenyang; Moon, Byung-Jo; Kim, Jung-Eun; Kim, Woomi; Hwang, Sung-Kyu; Yoon, Young‐Ran

doi:10.1365/s10337-008-0669-4

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…Recently, numerous chromatographic methods have been reported for the quantification of levofloxacin in body fluid samples, including traditional high-performance liquid chromatography with ultraviolet detection (HPLC-UV) [7][8][9][10], high-performance liquid chromatography with fluorescence detection (HPLC-FL) [11,12], high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/ MS) [13,14], ultra-high-performance liquid chromatography with ultraviolet detection (UHPLC-UV) [2,15], and ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) [16].…”

Section: Introductionmentioning

confidence: 99%

Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma

Meng

Wang

2019

Journal of Chemistry

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Fluoroquinolones are considered as gold standard for the prevention of bacterial infections. To improve assessment of antibacterial efficacy, a novel method for determination of levofloxacin was developed and validated. Deep eutectic solvents (DESs) as only green solvent were used as a porogen for preparation of water-compatible molecularly imprinted polymers (MIPs) with a pseudotemplate. The DESs-MIPs were characterized in detail, including scanning electron microscope, nitrogen sorption porosimetry, and Fourier transform-infrared spectra. Clearly, the maximum binding capacity of levofloxacin on DESs-MIPs in water and methanol was 0.216 and 0.077 μmol g−1, respectively. The DESs-MIPs as adsorbing materials were applied in microextraction by packed sorbent (MEPS), and the DESs-MIPs-MEPS conditions were optimized. The DESs-MIPs-MEPS coupled with ultra-high-performance liquid chromatography (UHPLC) was used to determine levofloxacin in human plasma. The method was found linear over 0.05–10 μg mL−1 with coefficient of correlation equal to 0.9988. The limit of detection and limit of quantification were 0.012 and 0.04 μg mL−1, respectively. At three spiked levels, the precision of proposed method was between 95.3% and 99.7% with intraday and interday relative standard deviations ≤8.9%. Finally, the developed method was used to examine levofloxacin from human plasma of 20 hospitalized patients after transrectal ultrasound-guided prostate biopsy, and the average concentration (±SD) of levofloxacin was 2.35 ± 0.99 μg mL−1 in plasma.

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Section: Introductionmentioning

confidence: 99%

Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma

Meng

Wang

2019

Journal of Chemistry

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“…Numerous analyticalstudieshavebeen reported for the determination of levofloxacin in biologicalsamples. Asurveyof thesepapers revealed thatsome of them are quitecomplexand lengthy,mostlybecauseo fthe sample preparation,like solid phasee xtraction [7], liquidliquid extraction [8,9], protein precipitation [10][11][12][13]. The liquid chromatographymass spectrometry method (LC-MS/MS)hasbeen utilized todetermine levofloxacin in humanp lasma [ 14].However,a ll thesem ethodss ufferfrom anumberof disadvantages,including tedious, time consuming sample preparation and high cost for routine analysis.…”

Section: Introductionmentioning

confidence: 99%

Bioequivalence study of levofloxacin tablets in healthy Indian volunteers using HPLC

Das

Mukherjee

Dey

et al. 2011

Arzneimittelforschung

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An improved HPLC method was developed and validated for the determination of concentration of levofloxacin (CAS 100986-85-4) in human plasma. This paper is an attempt to compare the bioavailability of two levofloxacin tablet formulations (reference and test) containing 500 mg of levofloxacin. Both the formulations were administered orally as a single dose, separated by a washout period of 1 week. The HPLC method was validated by examining the precision and accuracy for the inter-day and intra-day runs in a linear concentration range of 0.10-10.00 microg/ml. Bioequivalence of two formulations were determined in 12 healthy, Indian, male volunteers in a single-dose, two-period, two-sequence, two-treatment crossover study. The content of levofloxacin in plasma was determined using HPLC with UV detection. The formulations were compared using the following pharmacokinetic parameters: area under the plasma concentration-time curve (AUC(o-t)), area under the plasma concentration-time curve from zero to infinity (AUC(o-infinity)), peak plasma concentration (C(max)), and time to reach peak plasma concentration (t(max)). The results indicated that there were no statistically significant differences (P > 0.05) between the logarithmically transformed AUC(o-infinity), and C(max) values of the test and reference formulations. The 90% confidence interval for the ratio of the logarithmically transformed AUC(o-t), AUC(o-infinity) and C(max) were within the bioequivalence limit of 0.8-1.25 and the relative bioavailability of the test formulation was 99.98% of that of the reference formulation.

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“…UPLC techniques have been explored for applications in pharmacokinetic analyses [21], drug metabolism [22], and metabolite profiling [23]. To the best of our knowledge, the use of UPLC-MS/MS has not been demonstrated for pharmacokinetic studies of finasteride.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of finasteride in human plasma by UPLC–MS/MS and its application to clinical pharmacokinetic study

Phapale¹,

Lee²,

Lim³

et al. 2010

Journal of Chromatography B

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An Improved UPLC Method for Rapid Analysis of Levofloxacin in Human Plasma

Cited by 10 publications

References 16 publications

Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma

Microextraction by Packed Molecularly Imprinted Polymer Combined Ultra-High-Performance Liquid Chromatography for the Determination of Levofloxacin in Human Plasma

Bioequivalence study of levofloxacin tablets in healthy Indian volunteers using HPLC

Rapid determination of finasteride in human plasma by UPLC–MS/MS and its application to clinical pharmacokinetic study

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