An improved transformation protocol for studying gene expression in hairy roots of sugar beet ( Beta vulgaris L.)

Kifle, Sirak; Shao, Mingming; Jung, Christian; Cai, Daguang

doi:10.1007/s002990050614

Cited by 44 publications

(31 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the investigation reported here, we confirmed the insertion of the TL gene into potential transformants of G. littoralis. The successful A. rhizogenes-mediated transformation of chilli pepper (Yamakawa et al 1998) and sugar beet (Kifle et al 1999) seemed to depend on several factors, including cultivated variety, plant parts, bacteria condition, co-inoculation of bacteria and acetosyringone treatment (Benoit et al 1993;Mian 1993). The antibiotics used for bacterial elimination are possibly the most important factor.…”

Section: Discussionmentioning

confidence: 98%

Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation

2004

View full text Add to dashboard Cite

We investigated five penicillin derivatives that are popularly used for transformation experiments with Agrobacterium rhizogenes-penicillin G, carbenicillin, ampicillin, amoxicillin and cephalexin-for their effects on the growth and morphology of Beta vulgaris, Capsicum annuum and Glehnia littoralis roots. Attention was given to the relationship between their chemical structures and functions. Ampicillin was found to stimulate root elongation but inhibit root branching, whereas carbenicillin inhibited root elongation but promoted root branching. Root cultures were also exposed to hydrolyzed products of these antibiotics-i.e. phenylmalonic acid (PM), phenylglycine and 6-aminopenicillanic acid (6-APA): PM inhibited root elongation the most, while root elongation was supported best by 6-APA. These results indicate that both the side chains and the major component of penicillin derivatives affect root development and that the nature of the side chains is responsible for the responses. Ampicillin but not carbenicillin was used in subsequent experiments described herein to eliminate bacteria and to support root growth of transformants of the recalcitrant plants.

show abstract

Section: Discussionmentioning

confidence: 98%

Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation

2004

View full text Add to dashboard Cite

show abstract

“…In general, hairy root cultures have been used extensively in root nodule research (Diaz et al 1989;Quandt et al 1993;Diouf et al 1995;Hu and Du 2006;Hirotaka and Hiroshi 2003;Aarrouf et al 2012), production of artificial seeds (Uozumi and Kobayashi 1997), plant secondary metabolites and proteins (Aarrouf et al 2012), plant breeding and plant improvement, experimental systems to study responses to chemicals (Downs et al 1994;Mugnier 1997), plant morphology and development (Bandyopadhyay et al 2007; Turgut- Kara and Ari 2008;Hasancebi et al 2011;Aarrouf et al 2012), detoxifing environmental pollutants (Rugh 2001), validate and analyze the functions of genes conferring resistance to root specific pathogens (Remeeus et al 1998;Hwang et al 2000;Alpizar et al 2006;Aarrouf et al 2012) and study interactions with other organisms such as nematodes (Kifle et al 1999), mycorrhizal fungi and root pathogens (Mugnier 1997;Christey 2001). Besides these sights, enhanced rooting in plants helps establishment or surviving transplant shocks or abiotic stress like drought, salinity and heavy metal stress (Bulgakov, 2008;Li et al 2011).…”

Section: Hairy Root Diseasementioning

confidence: 99%

“…The resistance genes of nematode have been studied through using hairy roots (Cai et al 1995;Remeeus et al 1998;Kifle et al 1999;Hwang et al 2000). Development of plants using hairy roots have become of interest because of great potential for building up tolerance to biotic stresses and abiotic stresses (Porter 1991).…”

Section: A Rhizogenes and Crop Biotechnologymentioning

confidence: 99%

Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

2013

View full text Add to dashboard Cite

The history of Agrobacterium-related plant biotechnology goes back for more than three decades with the discovery of molecular mechanisms of crown gall disease in plants. After 1980s, gene technologies began developing rapidly and today, related with the improved gene transfer methods, plant biotechnology has become one of the most important branches in science. Till now, the most important genes related with agricultural affairs have been utilized for cloning of plants with the deployment of different techniques used in genetic engineering. Especially, Agrobacterium tumefaciens was used extensively for transferring desired genetic materials to plants rapidly and effectively by the researchers to create transgenic plants. Recognition of the biology of Agrobacterium species and newly developed applications of their T-DNA systems has been a great step in plant biotechnology. This chapter provides the reader with extensive information on A. rhizogenes which is responsible for the development of hairy root disease in a wide range of dicotyledonous plants and its T-DNA system. This knowledge will be useful in improving utilization of crops and the formulation of new and up-graded transgenic based food products.

show abstract

“…The sugar beet clone 93161p, kindly provided by the breeding company A. Dieckmann-Heimburg (Nienstädt, Germany), was used for the generation of transgenic hairy roots, according to Kifle et al (1999). In brief, leaf stalks were sterilised by submerging in calcium hypochloride (5%) for 10 min, followed by treatment with 70% ethanol for 5 min and repeated washing in sterile double distilled H 2 O.…”

Section: Plant Materials and Plant Transformationmentioning

confidence: 99%

Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.)

Menzel

Harloff

Jung

2003

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

Three genes from Ralstonia eutropha necessary for poly(3-hydroxybutyrate) (PHB) synthesis were introduced into the hairy roots of sugar beet. Transformation of a vector construct harbouring the PHB genes, each fused to the coding region of the pea ribulose-bisphosphate carboxylase plastid targeting sequence, resulted in 20 transgenic hairy-root clones, producing up to 55 mg high molecular PHB/g dry weight, as identified by gas chromatography, gel permeation chromatography and HPLC. Accumulation of PHB polymer in sugar beet root leucoplasts was confirmed by transmission electron microscopy. Thus, for the first time, plastidic PHB production was demonstrated for roots of a carbohydrate-storing crop plant.

show abstract

An improved transformation protocol for studying gene expression in hairy roots of sugar beet ( Beta vulgaris L.)

Cited by 44 publications

References 28 publications

Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation

Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation

Agrobacterium rhizogenes-Mediated Transformation and Its Biotechnological Applications in Crops

Expression of bacterial poly(3-hydroxybutyrate) synthesis genes in hairy roots of sugar beet (Beta vulgaris L.)

Contact Info

Product

Resources

About