Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation

Rahman, Laiq ur; Ikenaga, Toshihiko; Kitamura, Yoshihisa

doi:10.1007/s00299-003-0731-5

Cited by 22 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amoxicillin in the concentration of 400 mg/l even stimulated the embryogenic tissue growth of rapidly growing lines 13 and 30. Similar effects of amoxicillin on root growth during transformation of taxonomically different recalcitrant plants already in the concentration of 250 mg/l (with still preserved antibacterial efficiency) were demonstrated (ur Rahman et al 2004). Ticarcillin and carbenicillin were far less tolerated and their higher concentrations even reduced the embryogenic tissue growth in all lines.…”

Section: Discussionmentioning

confidence: 62%

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Malá¹,

Pavingerová²,

Cvrčková³

et al. 2009

J. For. Sci.

View full text Add to dashboard Cite

Somatic embryogenesis is conveniently utilized for the preparation of Norway spruce (Picea abies [L.] Karst.) transgenic clones by means of Agrobacterium. The establishment of successful transformation protocol requires to determine the tolerance of growing embryogenic tissue to antibiotics in culture and selective media. In 5 Norway spruce lines (genotypes) differences in the tolerance of embryogenic tissues to penicillin antibiotics (amoxicillin, carbenicillin, and ticarcillin), carbapenem antibiotic (meropenem) used for the Agrobacterium growth prevention, and aminoglycoside antibiotic (kanamycin) used in selective media were determined. Of the penicillin derivatives, amoxicillin was optimally tolerated in all lines and, in addition, its highest concentration accelerated growth in more rapidly growing lines. Ticarcillin was similarly tolerated but no growth acceleration was observed in any line. As regards carbenicillin, only the lowest concentration was observed to be well tolerated by all lines whereas all concentrations of meropenem were well tolerated in all lines except for slowly growing line 28, the growth of which was retarded by the concentration of 20 mg/l. The aminoglycoside antibiotic kanamycin was well tolerated by the embryonic tissue of all lines in the concentration of 10 mg/l and less in the concentration of 25 mg/l. The concentrations of 50 mg/l and 100 mg/l appeared as intolerable in all lines. Toxicity of kanamycin manifested at first in the browning and later in the growth cessation of embryogenic tissue.

show abstract

Section: Discussionmentioning

confidence: 62%

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Malá¹,

Pavingerová²,

Cvrčková³

et al. 2009

J. For. Sci.

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was isolated from fresh root material with CTAB according to the previous report (Rahman et al, 2004). To detect the insertions of HCHL gene and the TL genes of Ri plasmid (Bouches and Camilleri, 1990), putative transformants were examined using specific 25-to-26-mer oligonucleotide primers for amplification of 631 bp and 670 bp fragments, respectively.…”

Section: Detection Of Gene Insertion Of Hchl/tl By Pcrmentioning

confidence: 99%

HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls

Rahman

Kouno

Hashiguchi

et al. 2009

Bioresource Technology

View full text Add to dashboard Cite

As part of a study to explore the potential for new or modified bio-product formation, Beta vulgaris (sugar beet) has been genetically modified to express in root-organ culture a bacterial gene of phenylpropanoid catabolism. The HCHL gene, encoding p-hydroxycinnamoyl-CoA hydratase/lyase , was introduced into Beta vulgaris under the control of a CaMV 35S promoter, using Agrobacterium rhizogenes LBA 9402. Hairy root clones expressing the HCHL gene, together with non-expressing clones, were analyzed and revealed that one expression-positive clone accumulated the glucose ester of p-hydroxybenzoic acid (pHBA) at about 14 % on a dry weight basis. This is the best yield achieved in plant systems so far. Determination of cell-wall components liberated by alkaline hydrolysis confirmed that the ratio of pHBA to ferulic acid was considerably higher in the HCHL-expressing clones, whereas only ferulic acid was detected in a non-expressing clone. The change in cell-wall components also resulted in a decrease in tensile strength in the HCHL-expressing clones.

show abstract

“…However, difficulty in obtaining transformants is often encountered with recalcitrant plants, which are often wild species. In such cases, the use of ampicillin for bacterial elimination is particularly helpful in establishing root clones, because ampicillin stimulates root elongation and facilitates the isolation of sterile roots (Rahman et al 2004). Previously, we established root clones of G. littoralis using A. rhizogenes LBA9402, but none of the clones grew without ampicillin (Rahman et al 2004).…”

mentioning

confidence: 99%

“…In such cases, the use of ampicillin for bacterial elimination is particularly helpful in establishing root clones, because ampicillin stimulates root elongation and facilitates the isolation of sterile roots (Rahman et al 2004). Previously, we established root clones of G. littoralis using A. rhizogenes LBA9402, but none of the clones grew without ampicillin (Rahman et al 2004). Hence, in the current study, 10 clones that had been randomly selected from 100 clones were maintained in 25 ml of Murashige and Skoog (1962) (MS) basal medium supplemented with 5 mg l Ϫ1 of indole-3-butyric acid (IBA) in a 100 ml Erlenmeyer flask at 25°C and 80 rpm in the dark, with subcultures every 6 weeks, as described for normal root cultures of G. littoralis (Ishikawa et al 2007).…”

mentioning

confidence: 99%

“…50 mg of fresh root material by using an RNeasy Plant Mini kit (Qiagen), according to the manufacturer's instructions. PCR and RT-PCR conditions are as described in a previous study by our research group (Rahman et al 2004), except for the oligonucleotide primers, which are listed in Table 1. PCR analysis showed that the bands corresponding to rolA, rolB TL , and rolC were detected in both Gl4 and Gl6. However, rolB TR was found in Gl4 but not in Gl6 ( Figure 3).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection

Terato

Ishikawa

Yamada

et al. 2011

Plant Biotechnology

View full text Add to dashboard Cite

The objective of this study was to determine a possible application of hairy roots as biotechnological producers of furanocoumarins, by establishing root clones of Glehnia littoralis after infection from Agrobacterium rhizogenes. None of the root clones grew under hormone-free conditions, but when cultured in the presence of indole-3-butyric acid, growth varied substantially across clones. Analysis of the insertion and expression of pathogenic rol (A, B TL , B TR , and C ) genes for 10 clones showed that the stable expression of rolC and irregular expression of rolB were detected in 2 clones, which exhibited active growth and higher furanocoumarin induction. Following ascorbic acid treatment, the productivity of xanthotoxin and bergapten in a root clone harbouring a pathogenic gene was 2.8 and 3.7 times greater than that recorded in wild-type roots, respectively. These results indicate that root cultures possessing pathogenic rol genes offer a potential means to produce furanocoumarins. Key words: Agrobacterium rhizogenes, furanocoumarin induction, Glehnia littoralis, root clone.Plant Biotechnology 28, 317-321 (2011) DOI: 10.5511/plantbiotechnology.11.0330a Metabolomics NoteAbbreviations: AsA, ascorbic acid; BMT, bergaptol O-methyltransferase; CTAB, cetyltrimethylammonium bromide; DMSO, dimethyl sulfoxide; DW, dry weight; HPLC, high performance liquid chromatography; IBA, indole-3-butyric acid; MeJA, methyl jasmonate, MS, Murashige and Skoog; PAL, phenylalanine ammonia-lyase; RT-PCR, reverse transcription-polymerase chain reaction; SA, salicylic acid; UBI, ubiquitin; WT, wild type; YE, yeast extract. This article can be found at http://www.jspcmb.jp/ Published online June 10, 2011 a wild-type (WT) root for 6 weeks. The number and length of roots formed from each root tip was counted each week, and the weight was measured at 6 weeks. Root segments started to form a callus during the initial 2 weeks, although the extent of callus formation varied across clones. Thereafter, root differentiation from the callus began, followed by root elongation, leading to differing extents of root propagation in the clones. In brief, 3 clones (Gl2, Gl33, and Gl37) were rich in callus, resulting in higher weights than that recorded in other roots (Figure 1), but fewer roots were produced than WT (Figure 2A). However, one clone (Gl6) produced the highest number of roots (31Ϯ5 per segment) after 6 weeks, with a value almost double that of WT roots (16Ϯ4 per segment; Figure 2A). The number of roots formed by other clones, including Gl4, was similar to that recorded for WT roots. With respect to root length, Gl6 formed the longest root (11.4Ϯ2.0 mm), followed by Gl4 (8.7Ϯ2.1 mm), while other clones, including WT (5.7Ϯ0.7 mm), showed similar growth after 6 weeks ( Figure 2B).Since differences among clones appeared to be associated with the nature of the T-DNA insert present in each individual root clone, the insertion and expression of T-DNA was analysed by PCR and RT-PCR, respectively. There are 2 types of T-DNA on Ri-pla...

show abstract

Penicillin derivatives induce chemical structure-dependent root development, and application for plant transformation

Cited by 22 publications

References 24 publications

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

Tolerance of Norway spruce (Picea abies [L.] Karst.) embryogenic tissue to penicillin, carbapenem and aminoglycoside antibiotics

HCHL expression in hairy roots of Beta vulgaris yields a high accumulation of p-hydroxybenzoic acid (pHBA) glucose ester, and linkage of pHBA into cell walls

Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection

Contact Info

Product

Resources

About