An improved technique for isolated perfusion of rat livers and an evaluation of perfusates

Mischinger, Hans Jörg; Walsh, Thomas; Liu, Tao; Rao, Prakash; Rubin, Randy S.; Nakamura, Kenjiro; Todo, Satoru; Starzl, Thomas E.

doi:10.1016/0022-4804(92)90028-x

Cited by 39 publications

(19 citation statements)

References 27 publications

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“…The set-up for isolated rat liver function studies was according to published methods with minor modifications ( Figure 1) (1,16). Oxygenated Krebs-Henseleit bicarbonate buffer solution was used for all perfusions.…”

Section: Perfusion Of Isolated Rat Liversmentioning

confidence: 99%

Curcumin Has Potent Liver Preservation Properties in an Isolated Perfusion Model

Chen

Johnston²,

Wu³

et al. 2006

Transplantation

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Section: Perfusion Of Isolated Rat Liversmentioning

confidence: 99%

Curcumin Has Potent Liver Preservation Properties in an Isolated Perfusion Model

Chen

Johnston²,

Wu³

et al. 2006

Transplantation

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“…BSA was not included in the original KHB solution. Although it has been shown that BSA in KHB may be toxic for hepatocytes (Mischinger et al 1992) and causes excessive foam formation during reperfusion, its use is still maintained. Other reperfusion solutions used are 3-(N-morpholino)propane sulphonic acid (MOPS)-buffered Ringers solution (Cheung et al 1996), rat blood diluted in KHB (Alexander et al 1995), modified physiological saline (Ahmed et al 2001) and culture media such as minimal essential medium (MEM).…”

Section: Iprl Set-upmentioning

confidence: 99%

The isolated perfused rat liver: standardization of a time-honoured model

Hart²,

et al. 2006

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SummaryFor many years, the isolated perfused rat liver (IPRL) model has been used to investigate the physiology and pathophysiology of the rat liver. This in vitro model provides the opportunity to assess cellular injury and liver function in an isolated setting. This review offers an update of recent developments regarding the IPRL set-up as well as the viability parameters that are used, with regards to liver preservation and ischaemia and reperfusion mechanisms.A review of the literature was performed into studies regarding liver preservation or liver ischaemia and reperfusion. An overview of the literature is given with particular emphasis on perfusate type and volume, reperfusion pressure, flow, temperature, duration of perfusion, oxygenation and on applicable viability parameters (liver damage and function).The choice of IPRL set-up depends on the question examined and on the parameters of interest. A standard technique is cannulation of the portal vein, bile duct and caval vein with pressure-controlled perfusion at 20 cm H 2 O (15 mmHg) to reach a perfusion flow of approximately 3 mL/min/g liver weight. The preferred perfusion solution is Krebs-Henseleit buffer, without albumin. The usual volume is 150-300 cm 3 , oxygenated to a pO 2 of more than 500 mmHg. The temperature of the perfusate is maintained at 371C. Standardized markers should be used to allow comparison with other experiments.Keywords Isolated perfused rat liver (IPRL); liver preservation; parameters; liver function; liver damage For many years, the isolated perfused rat liver (IPRL) model has been used to investigate the physiology and pathophysiology of the rat liver. This in vitro model provides the opportunity to assess cellular injury and liver function in an isolated setting.The IPRL model was first reported by Claude Bernard in 1855 (Gores et al. 1986).In the review about the IPRL written by Gores et al. in 1986, the authors stated that the model remained a valuable reperfusion model, although other methods such as the assessment of liver slices, cell cultures, cell suspensions and isolated organelles had emerged. To date, the IPRL provides valuable data in studies regarding liver physiology using new techniques in the field of molecular biology and genetics.In the field of liver preservation, the IPRL model has been used for, among others, assessment of ischaemia-reperfusion injury, metabolism of perfusate compounds, metabolism of ammonium and amino acids (Haussinger 1987), endothelial function using hyaluronic acid uptake (Reinders et al. 1996), oxygen consumption (Dahn et al.

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“…33,34 Reperfusion after 60 or 120 minutes of warm ischemia, resulted in an immediate efflux of LDH and AST from the liver in both cases and in a second increase of effluent enzyme activity after about 1 hour of reperfusion (Figs. 1, 2, and 5).…”

Section: -28mentioning

confidence: 99%

Decrease of ischemic injury to the isolated perfused rat liver by loop diuretics

et al. 1997

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of viability during hypoxic conditions is greatly reduced. 3,4Recent studies suggest a major role played by sodium We tried to verify this effect of sodium-free solutions in the in the pathogenesis of ischemic liver injury: in these isolated perfused rat liver as a more complex model. As sostudies, sodium-free media have been shown to offer prodium-free perfusions proved impossible because of extensive tection against hypoxic injury to isolated hepatocytes.vasoconstriction, we switched to the use of inhibitors of soAs sodium-free perfusions of the isolated rat liver proved dium transporters. Here, we present the effects of two inhibiimpossible because of extensive vasoconstriction, we astors of the Na 71% during 60 minutes of ischemia in controls to 148% caval vein was immediately divided distally to the renal veins. Thora-{ 80% after bumetanide application (P õ .05). Also, after cotomy was performed, and the suprahepatic vena cava inferior was 120 minutes of warm ischemia, LDH and aspartate ami-cannulated from the right atrium with a 14-gauge cannula (Braun). notransferase release were significantly decreased and Thereafter, the infrahepatic vena cava inferior was ligated proxibile flow increased by pretreatment with bumetanide. mally to the right renal vein. The liver was dissected free and transferred to the perfusion bath filled with 0.9% NaCl, so that it floated Thus, both furosemide and bumetanide showed a clear freely. After transfer, homogeneity of perfusion was ascertained by benificial effect on rat livers subjected to warm ischinjecting 0.3 mL trypan blue (Serva, Heidelberg, Germany; 1 mmol/ emia. These data suggest that one means by which so-L in 0.9% NaCl) into the portal vein. dium ions are accumulated during liver ischemia mightThe livers were perfused with 37ЊC Krebs-Henseleit bicarbonate be the Na sodium in the incubation medium is replaced by choline, loss glucamine or HCl. Controls for the latter perfusions: Krebs-Henseleit without bicarbonate). The livers were perfused using a roller pump (Gilson, Villiers Le Bel, France) in a nonrecirculating perfusion system.7 Flow rate was 0.1 mL/min/g rat weight, which equalled 3.0 toAbbreviations: LDH, lactate dehydrogenase; AST, aspartate aminotransferase; NMR, 3.5 mL/min/g liver weight and resulted in a portal pressure of 4-6 nuclear magnetic resonance; Tm(DOTP), Thulium 1,4,7,4,7,

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An improved technique for isolated perfusion of rat livers and an evaluation of perfusates

Cited by 39 publications

References 27 publications

Curcumin Has Potent Liver Preservation Properties in an Isolated Perfusion Model

Curcumin Has Potent Liver Preservation Properties in an Isolated Perfusion Model

The isolated perfused rat liver: standardization of a time-honoured model

Decrease of ischemic injury to the isolated perfused rat liver by loop diuretics

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