Curcumin Has Potent Liver Preservation Properties in an Isolated Perfusion Model

Chen, Changguo; Johnston, Thomas D.; Wu, Guanghan; Ranjan, Dinesh

doi:10.1097/01.tp.0000232674.52951.ff

Cited by 13 publications

(7 citation statements)

References 47 publications

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“…Chen and co-workers found that curcumin has inherent organ preservation qualities in isolated perfusion models as it enhanced liver preservation in phosphate buffer saline (PBS) (Chen et al, 2006). In our study, flushing with CDC after 4 h of preservation showed that this compound can still be taken up by the liver cells, thus providing further support for its candidacy for use in a rinse solution after simple cold storage and just before transplantation.…”

Section: Discussionsupporting

confidence: 65%

“…The results of our studies showed that the application of different models of organ preservation did not apparently affect the tissue level of curcumin, especially after a period of 60 min of in situ warm ischemic damage. Chen and co‐workers found that curcumin has inherent organ preservation qualities in isolated perfusion models as it enhanced liver preservation in phosphate buffer saline (PBS) (Chen et al, ). In our study, flushing with CDC after 4 h of preservation showed that this compound can still be taken up by the liver cells, thus providing further support for its candidacy for use in a rinse solution after simple cold storage and just before transplantation.…”

Section: Discussionmentioning

confidence: 99%

“…Animal and clinical studies have demonstrated that reactive oxygen species, pro‐inflammatory cytokines and chemokines, and immune cells predispose allografts to increased IRI, and increase their primary dysfunction and rejection (Abu‐Amara et al, ; Liu, Fang, Wei, Dirsch, & Dahmen, ; Patel, Wilson, O'Dea, & Takata, ; Zhang et al, ). Liver preservation between donor operation and recipient operation is an important step in liver transplantation and new organ preservation solutions have been formulated to prevent or reduce the aforementioned factors (Chen, Johnston, Wu, & Ranjan, ). To protect as much as possible the quality of the organ during the storage period prior to transplantation several pharmacological options to tackle IRI have been developed (Balzan et al, ; de Rougemmont, Dutkowski, & Clavien, ; Thuillier et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…2013; Zhang et al, 2014). Liver preservation between donor operation and recipient operation is an important step in liver transplantation and new organ preservation solutions have been formulated to prevent or reduce the aforementioned factors (Chen, Johnston, Wu, & Ranjan, 2006). To protect as much as possible the quality of the organ during the storage period prior to transplantation several pharmacological options to tackle IRI have been developed (Balzan et al, 2014;de Rougemmont, Dutkowski, & Clavien, 2010;Thuillier et al, 2014).…”

mentioning

confidence: 99%

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Hepatocellular uptake of cyclodextrin‐complexed curcumin during liver preservation: A feasibility study

Saidi

Meurisse

Jochmans

et al. 2017

Biopharm & Drug Disp

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The increasing demand for donor organs and the decreasing organ quality is prompting research toward new methods to reduce ischemia reperfusion injury (IRI). Several strategies have been proposed to protect preserved organs from this injury. Before curcumin/dextrin complex (CDC), a potent antioxidant and anti-inflammatory agent, can be used clinically we need to better understand the intracellular uptake under hypothermic conditions on a rat model of liver donation after circulatory death (DCD) and brain death (DBD). To be able to use the fluorescence of CDC for quantification the stability of CDC in different preservation solutions at 4°C or 37°C was investigated. Livers from Wistar rats were procured after being flushed-out through the portal vein using CDC-enriched preservation solutions and stored at 4°C for variable periods. The CDC signal was stable in different preservation solutions over a period of 4 h and allowed the rapid and lasting uptake of curcumin into cells. After 4 h of preservation, CDC was no longer visible microscopically, and HPLC analysis showed very low to non-detectable tissue levels of CDC, proving metabolization during preservation. However, the distribution of CDC was not affected by warm ischemia damage (p = 0.278) nor by flushing the livers before or after 4 h of cold storage and without a warm preflush. Finally, curcumin reduced oxidative stress, lowered histological injury and did not change gene expression after WI/cold storage. Therefore, the use of CDC flush solution for the initial organ flush can offer a promising approach to the enhancement of liver preservation and the maintenance of its quality.

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Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Hepatocellular uptake of cyclodextrin‐complexed curcumin during liver preservation: A feasibility study

Saidi

Meurisse

Jochmans

et al. 2017

Biopharm & Drug Disp

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“…A large number of membrane and extracellular antioxidant agents have been tested experimentally to counterbalance the endogenous enzymatic depletion and improve liver graft initial function [11]–[15]. Other strategies used intracellular antioxidants components with controversial results [10], [16]–[18].…”

Section: Introductionmentioning

confidence: 99%

Pretreatment with Mangafodipir Improves Liver Graft Tolerance to Ischemia/Reperfusion Injury in Rat

et al. 2012

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Ischemia/reperfusion injury occurring during liver transplantation is mainly due to the generation of reactive oxygen species (ROS) upon revascularization. Thus, delivery of antioxidant enzymes might reduce the deleterious effects of ROS and improve liver graft initial function. Mangafodipir trisodium (MnDPDP), a contrast agent currently used in magnetic resonance imaging of the liver, has been shown to be endowed with powerful antioxidant properties. We hypothesized that MnDPDP could have a protective effect against liver ischemia reperfusion injury when administrated to the donor prior to harvesting. Livers from Sprague Dawley rats pretreated or not with MnDPDP were harvested and subsequently preserved for 24 h in Celsior® solution at 4°C. Organs were then perfused ex vivo for 120 min at 37°C with Krebs Henseleit solution. In MnDPDP (5 µmol/kg) group, we observed that ATP content was significantly higher at the end of the cold preservation period relative to untreated group. After reperfusion, livers from MnDPDP-treated rats showed better tissue integrity, less hepatocellular and endothelial cell injury. This was accompanied by larger amounts of bile production and higher ATP recovery as compared to untreated livers. The protective effect of MnDPDP was associated with a significant decrease of lipid peroxidation, mitochondrial damage, and apoptosis. Interestingly, MnDPDP-pretreated livers exhibited activation of Nfr2 and HIF-1α pathways resulting in a higher catalase and HO-1 activities. MnDPDP also increased total nitric oxide (NO) production which derived from higher expression of constitutive NO synthase and lower expression of inducible NO synthase. In conclusion, our results show that donor pretreatment with MnDPDP protects the rat liver graft from cold ischemia/reperfusion injury and demonstrate for the first time the potential interest of this molecule in the field of organ preservation. Since MnDPDP is safely used in liver imaging, this preservation strategy holds great promise for translation to clinical liver transplantation.

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Phytotherapy for treatment of H.pylori-induced chronic superficial gastritis in cases of chemotherapy intolerance

Hadji-Minaglou

2008

Phytothérapie

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Il est aujourd'hui admis que la bactérie Helicobacter pylori est l'agent étiologique des pathologies gastroduodénales. Elle développe une forte réponse inflammatoire locale et humorale qui s'installe progressivement dans la chronicité. Pour éliminer l'infection, le consensus en France est d'utiliser une trithérapie de sept jours associant un inhibiteur puissant de l'acidité gastrique et deux antibiotiques. Il a été démontré que le taux d'éradication diminue fortement lors d'une mauvaise observance du traitement. Nous avons étudié la possibilité d'un traitement alternatif au protocole du consensus lors d'une intolérance du patient au traitement. Notre choix s'est naturellement porté vers l'utilisation d'extraits végétaux. Cette étude présente les différentes plantes et formules retenues et les résultats préliminaires.Abstract: It is generally recognised today that the Helicobacter pylori bacterium is the cause of gastroduodenal disorders. It provokes significant local and humoral inflammation which gradually becomes chronic. Elimination of the infection is generally achieved in France via a 7 day course of tritherapy associating a powerful gastric acid inhibitor with two antibiotics. It has been shown that successful eradication depends on the patient following the treatment carefully. We have studied the option of an alternative to the generally accepted protocol for cases of intolerance to the treatment. Plant extracts appeared to us to be an obvious choice. This study presents the various plants and formulae examined and preliminary results.

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Curcumin Has Potent Liver Preservation Properties in an Isolated Perfusion Model

Abstract: We found that curcumin has inherent organ preservation quality as it enhanced liver preservation in PBS. In addition, curcumin enhanced the preservation quality of EC and UW solutions, thereby extending the preservation time while maintaining the organ quality.

Cited by 13 publications

References 47 publications

Hepatocellular uptake of cyclodextrin‐complexed curcumin during liver preservation: A feasibility study

Hepatocellular uptake of cyclodextrin‐complexed curcumin during liver preservation: A feasibility study

Pretreatment with Mangafodipir Improves Liver Graft Tolerance to Ischemia/Reperfusion Injury in Rat

Phytotherapy for treatment of H.pylori-induced chronic superficial gastritis in cases of chemotherapy intolerance

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