An improved surface for enhanced stem cell proliferation and osteogenic differentiation using electrospun composite PLLA/P123 scaffold

Abstract

Poly-L-lactic acid (PLLA) nano fibrous scaffolds prepared by electrospinning technology have been used widely in tissue engineering applications. However, PLLA scaffolds are hydrophobic in nature, moreover the fibrous porous structure produced by electrospinning makes the scaffolds even more hydrophobic which generally limits cell attachment and proliferation. Polymer blending is one of the several efforts used so far to enhance hydrophilicity and recognized as an easy cost-effective approach for the manipulation physiochemical properties of polymeric biomaterials. Pluronic block copolymers containing hydrophilic poly(ethylene oxide) (PEO) blocks and hydrophobic poly(propylene oxide) (PPO) blocks are arranged in triblock structure: PEO-PPO-PEO. It is commonly used recently to blend hydrophobic polymers to enhance hydrophilicity for pharmaceutical and tissue engineering applications. In this study, novel pluronic P123 blend PLLA electrospun nanofibre scaffolds with improved hydrophilicity and biological properties were fabricated. The surface morphology and surface chemistry of the nanofibre scaffolds were characterized by scanning electron microscope (SEM) and FTIR analyses. Surface hydrophilicity and change in mechanical properties were studied. The ability of the scaffolds to support the attachment, and proliferation and differentiation of human adipose tissue derived MSCs, were evaluated generally. The fabricated scaffolds have completely improved, hydrophilicity, similar osteogenic differentiation potential with plasma-treated PLLA nanofibre scaffold, and hence P123 blend PLLA electrospun nanofibre scaffolds are a very good and cost effective choice as a scaffold for bone tissue engineering application.

Enhanced osteogenic differentiation of mesenchymal stem cells on metal–organic framework based on copper, zinc, and imidazole coated poly‐l‐lactic acid nanofiber scaffolds

“…To determine the resistance of scaffolds against imposed stresses during in vitro cell culture and in vivo implantation, their tensile strength should be evaluated (Shao, Chen, Wang, Chen, & Du, 2012). It usually peaks after 5-14 days, depending on cell sources and culture conditions (Birhanu et al, 2018). As Cell attachment and proliferation result by MTT assay showed, coating of MOF over pure PLLA showed good biocompatibility of the scaffolds and provided favorable conditions for adhesion, spreading and proliferation of cells.…”

“…It increases, but then decreases when the culture becomes well-mineralized. It usually peaks after 5-14 days, depending on cell sources and culture conditions (Birhanu et al, 2018). ALP has a critical role in bone matrix mineralization through cleavage of phosphate group and its elevated activity is observed before the initiation of calcium minerals.…”

“…The pale green precipitate was collected by filtration, washed several times with Deionized (DI) water followed drying at 105 C. The nanofibers scaffolds were obtained by electrospinning as described previously (Birhanu, Akbari Javar, Seyedjafari, Zandi-Karimi, & Dusti Telgerd, 2018). First, PLLA scaffolds were made as follows: 0.43 g of PLLA (7% w/v) was dissolved in 6.0 mL of chloroform wile stirring till a clear solution was appeared.…”

Three-dimensional nanofiberous PLLA/PCL scaffold improved biochemical and molecular markers hiPS cell-derived insulin-producing islet-like cells

“…More importantly, L-lactic acid is the final product of PLLA scaffolds degradation which is harmless for the body [33]. Moreover the fibrous porous structure produced by blending is one of the several efforts used to enhance Polymer hydrophilicity [34] that introduced them as a good candidate for tissue engineering applications, stem cell proliferation and differentiation. Moreover, hybrid scaffold is energy-cost and labor saving without having the disadvantages of single polymer [35] Many studies show that embryonic cells and hiPSCs can differentiate into beta like-cells by cytokine growth factors, microRNA and on gelatin-coated plates or nanofibrous scaffolds, but so far, sufficient studies on differentiation of hiPSCs to pancreatic islet cells on the surface of nano-scaffold hybrids have not been performed.…”

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

“…The ability of the scaffolds to support the attachment, and proliferation and differentiation of human adipose tissue derived MSCs, were evaluated generally. They believe that P123 blend PLLA electrospun nanofibrous scaffolds are a very good and cost effective choice as a scaffold for bone tissue engineering applications (Birhanu, Akbari Javar, Seyedjafari, Zandi‐Karimi, & Dusti Telgerd, ). Poor supply of growth factors is one the main concerning of regenerative medicine scientists.…”