An improved non-enzymatic electrochemical sensor amplified with CuO nanostructures for sensitive determination of uric acid

Abstract

This study displays the facile and fluent electrochemical determination of uric acid (UA) through exceptional copper oxide nanostructures (CuO), as an effective sensing probe. The copper oxide nanostructures were fabricated via an aqueous chemical growth method using sodium hydroxide as a reducing agent, which massively hold hydroxide source. Copper oxide nanostructures showed astonishing electrocatalytic behavior in the detection of UA. Different characterization techniques such as XRD, FESEM, and EDS were exploited to determine crystalline nature, morphologies, and elemental composition of synthesized nanostructures. The cyclic voltammetry (CV) was subjected to investigate the electrochemical performance of UA using copper oxide nanostructures modified glassy carbon electrode CuO/GCE. The CV parameters were optimized at a scan rate of 50 mV/s with −0.7 to 0.9 potential range, and the UA response was investigated at 0.4 mV. PBS buffer of pH 7.4 was exploited as a supporting electrolyte. The linear dynamic range for UA was 0.001–351 mM with a very low limit of detection observed as 0.6 µM. The proposed sensor was successfully applied in urine samples for the detection of UA with improved sensitivity and selectivity.

Microporous carbon in the selective electro-oxidation of molecular biomarkers: uric acid, ascorbic acid, and dopamine

“…The limits of detection obtained in this work are significantly lower than the levels of AA, DA, and UA anticipated in human urine (AA: 458–9602 μM, 67 DA: 0.3–3.13 μM, 68 UA: 149–416 μM). 69 The developed method will thus be useful for the monitoring of the three molecules in bodily fluids. The analytical performance of the developed method is compared with other previous electrochemical sensors for AA, DA, and UA detection in Table 1.…”

“…The low, at, and reproducible baseline current at the 10 mV pulse amplitude was also preferable for electrochemical measurement and was thus chosen for the analysis. 69 The developed method will thus be useful for the monitoring of the three molecules in bodily uids. The analytical performance of the developed method is compared with other previous electrochemical sensors for AA, DA, and UA detection in Table 1.…”

Developing Activated Carbon Veil Electrode for Sensing Salivary Uric Acid

“…Еp (V) = (0.770 ± 0.08) − (0.055 ± 0.007) рН, R 2 = 0.953 (2) According to [20][21][22]38], two electrons and two protons participate in UA electrooxidation (Scheme 1).…”

“…The slope of Equation (2) happened to be 0.055 V per pH unit, which suggests that the number of protons and electrons involved in the process of electrooxidation is equal. E p (V) = (0.770 ± 0.08) − (0.055 ± 0.007) pH, R 2 = 0.953 (2) According to [20][21][22]38], two electrons and two protons participate in UA electrooxidation (Scheme 1).…”

“…Most sensors have a modifying composite layer that may contain various materials. Sometimes the process of creating a multicomponent modifier and composite layers is multi-staged and time-consuming [19][20][21][22] and requires the use of harmful solvents [21,23]. In this regard, the search for original approaches and new electrically conductive materials with highly developed active surfaces, which can be used for creating simple and highly sensitive sensors for UA determination is relevant.…”