An Improved Micromethod for Vancomycin Determination by High-Performance Liquid Chromatography

Li, Li; Miles, Michael V.; Hall, William R.; Carson, Stanley W.

doi:10.1097/00007691-199508000-00009

Cited by 24 publications

(26 citation statements)

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“…Solid-phase extraction, as used by Greene et al (1987), was replaced by a less timeconsuming method of sample preparation. The LLOQ found in plasma conforms to other LLOQs reported for vancomycin in bioanalytical assays using HPLC with UV detection (Farin et al, 1998;Furuta et al, 2000) or was even lower (Li et al, 1995;Greene et al, 1987;Backes et al, 1998;Demotes-Mainard et al, 1994;Hu et al, 1990;Luksa and Marusic, 1995). More sensitive methods have been reported using tandem mass spectrometry for detection (Cass et al, 2001;Shibata et al, 2003).…”

Section: Resultssupporting

confidence: 82%

“…Previously reported bioanalytical methods for vancomycin determine the concentration in plasma/ serum (Li et al, 1995;Cass et al, 2001;Kitahashi and Furuta, 2001;Shibata et al, 2003), urine samples (Cass et al, 2001) or haemofiltrate (Uchino et al, 2002). We here report the development of a high-performance liquid chromatography (HPLC) method for microdialysate and plasma.…”

Section: Introductionmentioning

confidence: 99%

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Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Plock¹,

Buerger²,

Kloft³

2005

Biomed. Chromatogr.

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Vancomycin is a glycopeptide antibiotic approved for the treatment of serious infections or patients allergic to beta-lactams. A rapid HPLC assay using UV detection for the determination in microdialysate and human plasma was developed. After sample preparation, using methanol and trichloroacetic acid for plasma and water for microdialysate, 20 microL were injected and separated on a RP(18) column. Overall, the assay exhibited good precision and accuracy. The diffusion properties of vancomycin investigated in in vitro microdialysis experiments revealed an unfavourable concentration dependence avertable by keeping a constant pH using phosphate buffer as perfusate. The mean relative recoveries were 27.8% [coefficient of variation (CV) 11.1%] and 33.2% (CV 8.3%) for retrodialysis and recovery experiments, respectively. Following characterization of vancomycin in in vitro microdialysis, the developed setting is suitable for application in (pre-)clinical studies.

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Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Plock¹,

Buerger²,

Kloft³

2005

Biomed. Chromatogr.

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“…The determination of vancomycin is possible in different types of biological fluids. Most published methods are based on the analysis of vancomycin in human or animal serum , plasma , and urine samples . However, LC methods also enable the measurement of vancomycin in the cerebrospinal fluid bronchoalveolar lavage (BAL) fluid , artificial perfusion fluid, lung tissue samples , tissues, bone samples and vitreous and aqueous humor .…”

Section: Sample Pre‐treatmentmentioning

confidence: 99%

“…On the other hand, a suitable approach is the application of LLE after protein precipitation . For protein precipitation in both cases , perchloric acid was added to the plasma or serum sample. After centrifugation, the supernatant was extracted with a non‐polar organic solvent.…”

Section: Sample Pre‐treatmentmentioning

confidence: 99%

Modern methods for vancomycin determination in biological fluids by methods based on high‐performance liquid chromatography – A review

Javorská

Krcmova

Solichová

et al. 2015

J of Separation Science

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Vancomycin is a glycopeptide antibiotic used in the therapy of severe bacterial infection. The monitoring of vancomycin levels is recommended because of its narrow therapeutic index and toxicity. This measurement is especially appropriate in patients with unstable renal functions, who receive high doses of vancomycin or present serious bacterial infections accompanied by important sequestration of liquids when it could be difficult to achieve the optimal therapeutic dose. Most of the methods for vancomycin determination in routine practice are immunoassays. However, chromatography-based techniques in combination with UV or mass spectrometry detection provide results with greater accuracy and precision also in complicated biological matrices. This review provides a detailed overview of modern approaches for the chromatographic separation of vancomycin in various biological samples and useful sample preparation procedures for vancomycin determination in various biological fluids.

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“…In the clinical laboratory the use of this technique has been efficient for determination of analytes in several biological fluids. Therefore, HPLC has been developed for the analysis of vancomycin in medicines, as well as for the determination in biological fluids 17,24,[26][27][28][29][30][31][32][33][34] . Kitahashi and Furuta 35 developed an assay for measuring vancomycin concentration by micellar electrokinetic capillary chromatography with detection at 210 nm using direct serum injection.…”

Section: Clinical Applicationmentioning

confidence: 99%

Untitled

Tariq¹,

Siddiqui²,

Kumar³

et al. 2010

ScienceAsia

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A reverse phase-liquid chromatographic method with UV detection at 280 nm is described for simultaneous determination of ceftriaxone sodium and vancomycin hydrochloride. Chromatographic separation of the two drugs was achieved on a Betasil C-1 column using a mobile phase consisting of a binary mixture of acetonitrile and triethylamine buffer adjusted to pH 3.5 ± 0.1 with orthophosphoric acid in a ratio of 20:80. The liquid chromatographic method developed offers symmetric peak shape, good resolution, and reasonable retention time for both drugs. Linearity, accuracy, and precision were found to be acceptable over the concentration ranges 125-750 ppm for ceftriaxone and 62.5-375 ppm for vancomycin. The liquid chromatographic method was successfully applied to the quality control of formulated products, plasma, and cerebrospinal fluid samples containing ceftriaxone and vancomycin.

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An Improved Micromethod for Vancomycin Determination by High-Performance Liquid Chromatography

Cited by 24 publications

References 0 publications

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Modern methods for vancomycin determination in biological fluids by methods based on high‐performance liquid chromatography – A review

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