An improved microbial assay for the detection of chloramphenicol residues in shrimp tissues

Shakila, R. Jeya; Saravanakumar, R.; Vyla, S. Aunto Princy; Jeyasekaran, G.

doi:10.1016/j.ifset.2007.03.002

Cited by 24 publications

(12 citation statements)

References 7 publications

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“…The most sensitive microbial inhibition test method was reported by Shakila et al (2007) with a sensitivity of 1 µg/kg for shrimp tissue, but this involved an extraction procedure using a 100-g sample.…”

Section: Screening Methodsmentioning

confidence: 99%

“…Microbial inhibition tests, such as the EC four-plate test (Lynas et al, 1998;Tajik et al, 2010) and the one-plate method (Koenen-Dierick et al, 1995) for chloramphenicol in tissue samples and some commercially available tests for chloramphenicol in milk samples (Althaus et al, 2003), are not sufficiently sensitive to determine chloramphenicol residues in test samples at the MRPL; the sensitivity of these methods for chloramphenicol was reported to be 300 µg/kg, 30 000 g/kg and 12 000 µg/kg, respectively. The most sensitive microbial inhibition test method was reported by Shakila et al (2007) with a sensitivity of 1 µg/kg for shrimp tissue, but this involved an extraction procedure using a 100-g sample.…”

Section: Screening Methodsmentioning

confidence: 99%

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Scientific Opinion on Chloramphenicol in food and feed

2014

EFS2

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Chloramphenicol is an antibiotic not authorised for use in food-producing animals in the European Union (EU). However, being produced by soil bacteria, it may occur in plants. The European Commission asked EFSA for a scientific opinion on the risks to human and animal health related to the presence of chloramphenicol in food and feed and whether a reference point for action (RPA) of 0.3 µg/kg is adequate to protect public and animal health. Data on occurrence of chloramphenicol in food extracted from the national residue monitoring plan results and from the Rapid Alert System for Food and Feed (RASFF) were too limited to carry out a reliable human dietary exposure assessment. Instead, human dietary exposure was calculated for a scenario in which chloramphenicol is present at 0.3 µg/kg in all foods of animal origin, foods containing enzyme preparations and foods which may be contaminated naturally. The mean chronic dietary exposure for this worst-case scenario would range from 11 to 17 and 2.2 to 4.0 ng/kg b.w. per day for toddlers and adults, respectively. The potential dietary exposure of livestock to chloramphenicol was estimated to be below 1 µg/kg b.w. per day. Chloramphenicol is implicated in the generation of aplastic anaemia in humans and causes reproductive/hepatotoxic effects in animals. Margins of exposure for these effects were calculated at 2.4 × 10 5 or greater and the CONTAM Panel concluded that it is unlikely that exposure to food contaminated with chloramphenicol at or below 0.3 µg/kg is a health concern for aplastic anaemia or reproductive/hepatotoxic effects. Chloramphenicol exhibits genotoxicity but, owing to the lack of data, the risk of carcinogenicity cannot be assessed. The SUMMARYChloramphenicol is a broad-spectrum antibiotic effective against Gram-positive and Gram-negative bacteria and, in the past, has been widely used to treat infections in both humans and animals. Chloramphenicol is not authorised for use in food-producing animals in the European Union (EU) but may be used in human medicine and in treatments for non-food-producing animals. Apart from its potential occurrence as a residue in food from illicit treatment of food-producing animals, chloramphenicol has also been used in feed and food enzyme products and may occur naturally in plants from its production by the soil bacterium Streptomyces venezuelae.The EFSA Scientific Opinion entitled "Guidance on methodological principles and scientific methods to be taken into account when establishing Reference Points for Action (RPAs) for non-allowed pharmacologically active substances present in food of animal origin" identified an approach for establishing RPAs for various categories of non-allowed pharmacologically active substances. However, the opinion also identified certain categories of non-allowed pharmacologically active substances that are considered to be outside the scope of the procedure, including substances causing blood dyscrasias (aplastic anaemia) such as chloramphenicol. As chloramphenicol is excluded fro...

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Section: Screening Methodsmentioning

confidence: 99%

Section: Screening Methodsmentioning

confidence: 99%

Scientific Opinion on Chloramphenicol in food and feed

2014

EFS2

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“…Shakila et al developed a microbial screening assay for the detection of CAP in shrimp after extraction with with EtOAc/NH4OH and ACN [230]. Luo et al…”

Section: Nitrofuransmentioning

confidence: 99%

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Kinsella

O’Mahony

Malone

et al. 2009

Journal of Chromatography A

179

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A comprehensive review is presented on the current trends in sample preparation for isolation of veterinary drugs and growth promotors from foods. The objective of the review is to firstly give an overview of the sample preparation techniques that are applied in field. The review will focus on new techniques and technologies, which improve efficiency and coverage of residues. The underlying theme to the paper is the developments that have been made in multi-residue methods and particularly multi-class methods for residues of licensed animal health products, which have been developed in the last couple of years. The role of multi-class methods is discussed and how they can be accommodated in future residue surveillance.

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“…European Community tries to uphold a high level of food standards to protect public health and safety7 and has set up minimum required performance level at 0.3 μg/kg for CAP in food of animal origin 8. Numerous methods such as enzyme linked immunosorbent assay,9 liquid chromatography (LC), and LC‐mass spectrometry (MS),10 gas chromatography‐mass spectrometry,11 microbial assays,12 capillary zone electrophoresis,13 chemiluminescence,14 biosensor‐based immunoassay,15 and immunoaffinity chromatography16 have been used for the determination of CAP in different samples. Although good results were obtained with the methods mentioned, more sensitive, rapid, and new detection techniques are still required for CAP determination.…”

Section: Introductionmentioning

confidence: 99%

Combining molecular imprinted nanoparticles with surface plasmon resonance nanosensor for chloramphenicol detection in honey

Kara

Uzun

Kolaylı

et al. 2013

J of Applied Polymer Sci

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The focus of this article is to develop a surface plasmon resonance (SPR) nanosensor to determine chloramphenicol (CAP) using the molecularly imprinted nanoparticles. The CAP imprinted nanoparticles were prepared by miniemulsion polymerization method. Then, the nanoparticles were attached onto the SPR nanosensor surface via temperature-controlled evaporation. Surface characterization studies were performed with atomic force microscopy and contact angle measurements. Kinetic studies were performed with CAP solutions in the concentration range of 0.155-6.192 nM. Florphenicol (FLP) and thiamphenicol (TAP) having similar chemical structures to the template (i.e., CAP) were chosen as competitors to determine selectivity of the nanoparticles. Selectivity constants were observed as 8.86 for CAP/TAP and 8.36 for CAP/FLP. The detection limit was calculated as 40 ng/kg honey sample. In the light of these results, it was emphasized that the SPR nanosensor is able to recognize CAP selectively and has a potential for realtime CAP detection in honey sample.

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An improved microbial assay for the detection of chloramphenicol residues in shrimp tissues

Cited by 24 publications

References 7 publications

Scientific Opinion on Chloramphenicol in food and feed

Scientific Opinion on Chloramphenicol in food and feed

Current trends in sample preparation for growth promoter and veterinary drug residue analysis

Combining molecular imprinted nanoparticles with surface plasmon resonance nanosensor for chloramphenicol detection in honey

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