An improved method to distinguish <i>Entamoeba histolytica</i> 
and <i>Entamoeba dispar</i>

Gomes, Maria Aparecida; Pesquero, João Bosco; Furst, Cinthia; Valle, Paulo Renato; Pesquero, Jorge L.; Silva, E. F.

doi:10.1017/s0031182099004886

Cited by 19 publications

(15 citation statements)

References 10 publications

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“…EGG strain was isolated from a patient with dysenteric colitis, hepatic abscess and positive serology. These samples were identified as E. dispar and E. histolytica by zymodeme and PCR (Martinez et al, 1996;Gomes et al, 1999). Trophozoites from each strain were thawed in water bath and maintained in Pavlova medium at 37 o C, with passages at each every three days.…”

Section: Ementioning

confidence: 99%

Morphometric study of the hepatic lesions experimentally induced in hamsters byEntamoeba disparandE. histolytica

Costa

Brito

Gomes³

et al. 2007

Parasite

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Summary :Evolution of experimental hepatic lesions produced in hamsters with Entamoeba histolytica and E. dispar was evaluated quantitatively and qualitatively through morphometry and immunohistochemistry. Animals infected with E. dispar developed hepatic lesions quantitatively and qualitatively similar to those produced by E. histolytica on the first three days of infection. On the 6 th and 8 th days of infection, E. histolytica produced larger tissue damage than E. dispar. A gradual decrease was observed in the number of trophozoites along the infection. A negative correlation was observed between the reduced number of trophozoites and the larger area of necrosis in both groups, confirming the importance of trophozoites killed in the lesion genesis. Regarding the genetic similarity between E. histolytica and E. dispar, comparison strategy between lesions produced by these species may culminate in identifying virulence factors of E. histolytica. Résumé : ÉTUDE MORPHOMÉTRIQUE DES LESIONS HÉPATIQUES INDUITES EXPÉRIMENTALEMENT CHEZ LES HAMSTERS

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Section: Ementioning

confidence: 99%

Morphometric study of the hepatic lesions experimentally induced in hamsters byEntamoeba disparandE. histolytica

Costa

Brito

Gomes³

et al. 2007

Parasite

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“…As soon as sufficient growth had occurred, trophozoites were grown in culture flasks to produce lysates for isoenzyme typing 22 . Genetic identification was also confirmed by polymerase chain reaction through analyses of conformational polymorphism in the 482 bp fragment, as previously described 14 . Trophozoites of each isolate were cloned using a dilution method 26 and the clones ADO-A3, ADO-B3 and MGL2-A3 were used in the experiments.…”

Section: Parasitesmentioning

confidence: 99%

“…Viable C. fasciculata was added only on the day of sub-culturing. Monoxenic samples were also submitted to isoenzyme typing and analysis of conformational polymorphism in the 482 bp fragment 14 .…”

Section: Parasitesmentioning

confidence: 99%

Pathogenicity of Entamoeba dispar under xenic and monoxenic cultivation compared to a virulent E. histolytica

Costa

Gomes

Rocha

et al. 2006

Rev. Inst. Med. trop. S. Paulo

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SUMMARYTwo xenic isolates and cloned cultures of Entamoeba dispar were submitted to monoxenization using Crithidia fasciculata as the associated organism. Growth in monoxenic cultivation and ability of xenic and monoxenic trophozoites to destroy VERO cells and produce lesions in hamster livers were compared to those of a virulent E. histolytica. Parental and cloned E. dispar under monoxenic cultivation showed a remarkable lower growth than the monoxenic E. histolytica and were avirulent in both in vivo and in vitro tests. When xenically cultured, trophozoites of E. dispar showed a moderate lytic activity against VERO cells (1.5 to 41.8% of destruction) but caused severe hepatic lesions in hamsters as those caused by the virulent E. histolytica (29 to 100% in prevalence and 0.86 to 4.00 in lesion degree). Although E. dispar has not been associated with invasive disease in men, the ability of xenic trophozoites to produce prominent tissue damage in experimental conditions has indicated that some strains have a considerable pathogenic potential when in presence of bacteria.

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“…Recent studies demonstrated that up to 90% of these patients are infected with the nonpathogenic species E. dispar and not E. histolytica (7,11,14,35). Because cysts of E. histolytica are morphologically similar to those of E. dispar, other techniques (e.g., PCR and ELISA for detection of specific antigens) are in use for differentiation of species from stools (8,32). Although often satisfactory, antigen detection can lack sensitivity, and PCR requires advanced laboratory infrastructure and specific knowledge, especially because commercial PCR tests for ame- Table 1.…”

Section: Discussionmentioning

confidence: 99%

“…histolytica and E. dispar PCR. PCR and subsequent polyacrylamide gel electrophoresis were performed as described previously by Gomes et al (8).…”

Section: Methodsmentioning

confidence: 99%

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

Doorn¹,

Hofwegen²,

Koelewijn³

et al. 2005

J Clin Microbiol

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A homemade enzyme-linked immunosorbent assay (ELISA) and a dipstick assay (Dipstick) for the detection of anti-Entamoeba histolytica antibodies in serum were developed and evaluated together with a commercially available latex agglutination test (LAT; Laboratoires Fumouze) for their use in serodiagnosis of amebiasis. The sensitivity of these assays was evaluated with sera from 27 patients with radiologically proven, cellulose acetate precipitation (CAP) test-positive amebic liver abscess, 7 patients with parasitologically and PCR-proven amebic colitis, and 11 patients with parasitologically and PCR-proven E. histolytica cyst passage. The sensitivities of the ELISA, Dipstick, and LAT were all 93.3% (42/45). With a combination of Dipstick and LAT, all abscess and colitis patients had at least one positive result. The specificity was assessed with 238 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases, sera containing autoimmune antibodies, and sera from healthy blood donors. The specificities of the ELISA, Dipstick, and LAT were 97.1%, 98.1%, and 99.5%, respectively. Of 61 sera from patients with PCR-proven E. dispar infection, 60 (98.4%) were negative in both Dipstick and LAT and 59 (96.7%) were negative in ELISA. Our data suggest that all three assays are sensitive serological tests. The rapid LAT and Dipstick provide fast results and can easily be applied in routine laboratories in order to facilitate the diagnosis of amebiasis.

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An improved method to distinguish Entamoeba histolytica and Entamoeba dispar

Cited by 19 publications

References 10 publications

Morphometric study of the hepatic lesions experimentally induced in hamsters byEntamoeba disparandE. histolytica

Morphometric study of the hepatic lesions experimentally induced in hamsters byEntamoeba disparandE. histolytica

Pathogenicity of Entamoeba dispar under xenic and monoxenic cultivation compared to a virulent E. histolytica

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

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