An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution

Štorchová, Helena; Hrdličková, Radmila; Chrtek, Jindřich; Tetera, Martin; Fitze, Dorothee; Fehrer, Judith

doi:10.2307/1223934

Cited by 179 publications

(108 citation statements)

References 5 publications

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“…To complete this data set, rbcL sequences were determined for 10 species (see Table S1). Total plant genomic DNA was extracted following a sorbitol DNA extraction method (Storchova et al, 2000) for Myosotis scorpioïdes species and following the CTAB (cetyltrimethyl ammonium bromide) method (Doyle & Doyle, 1990) for the other species. The rbcL gene was amplified using a PCR with the primers rbcL1F (5¢-ATGTCACCACAAACAGA-GACT-3¢) and rbcL1369R (5¢-TTCCATACTTCACAA-GCAGC-3¢).…”

Section: Genetic Data Acquisition and Phylogenetic Reconstructionmentioning

confidence: 99%

Plant resistance to mechanical stress: evidence of an avoidance–tolerance trade‐off

et al. 2011

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Summary• External mechanical forces resulting from the pressure exerted by wind or water movement are a major stress factor for plants and may cause regular disturbances in many ecosystems. A plant's ability to resist these forces relies either on minimizing the forces encountered by the plant (avoidance strategy), or on maximizing its resistance to breakage (tolerance strategy). We investigated plant resistance strategies using aquatic vegetation as a model, and examined whether avoidance and tolerance are negatively correlated.• We tested the avoidance-tolerance correlation across 28 species using a phylogenetically corrected analysis, after construction of a molecular phylogeny for the species considered.• Different species demonstrated contrasting avoidance and tolerance and we demonstrated a significant negative relationship between the two strategies, which suggests an avoidance-tolerance trade-off.• Negative relationships may result from costs that each strategy incurs or from constraints imposed by physical laws on plant tissues. The existence of such a trade-off has important ecological and evolutionary consequences. It would lead to constraints on the evolution and variation of both strategies, possibly limiting their evolution and may constrain many morphological, anatomical and architectural traits that underlie avoidance and tolerance.

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Section: Genetic Data Acquisition and Phylogenetic Reconstructionmentioning

confidence: 99%

Plant resistance to mechanical stress: evidence of an avoidance–tolerance trade‐off

et al. 2011

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“…Although the DNA extraction buffers assessed (based on the use of CTAB, SDS, mannitol and sorbitol) are established for different species (Ojeda, 2012;Mogg and Bond, 2003;Štorchová et al, 2000) they differ in their efficiency in obtaining C. linearifolius genomic DNA (p<0.05; Kruskal-Wallis test). Considering the concentrations estimated using the NanoDrop spectrometer, it is possible to identify at least three efficiency levels (p<0.05, SNK test).…”

Section: Resultsmentioning

confidence: 99%

Comparison of protocols for genomic DNA extraction from velame pimenta (Croton linearifolius), a native species to the Caatinga, Brazil

Scaldaferri¹,

Freitas²,

Santos³

et al. 2013

Afr. J. Biotechnol.

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The Caatinga biome occupies some 12% of the Brazilian territory, which is present in at least nine states. The species that constitute its biodiversity have the potential to be used as natural resources, among them are approximately 700 species of the genus Croton. As an example of this potential, the Croton linearifolius specie is used by local communities as a natural insecticide. Associated with the economic potential of the Caatinga species, one must stress the risk of extinction or genetic erosion due to the growing deforestation of natural areas of this biome. These factors make it relevant in genetic studies in order to guide conservation strategies. Considering the lack of molecular studies involving C. linearifolius, we compared the efficiency of six protocols for genomic DNA extraction previously described in literature. The DNA extraction buffers [based on the use of Cetyl trimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), mannitol and sorbitol] were different in their efficiency to obtaining the genomic DNA of C. linearifloius. In general, protocols using CTAB buffer were more efficient. The use of liquid nitrogen in the maceration process was also evaluated and its use was considered a no necessary factor in obtaining DNA in adequate quantity and quality for PCR platform procedures.

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“…Genomic DNA was isolated from leaves of transgenic and non-transgenic plants as previously described by Štorchová et al (2000) [19]. For each sample of DNA, 100 ng of genomic DNA in 25 µL volume was used per PCR reaction.…”

Section: Molecular Analysismentioning

confidence: 99%

<i>Agrobacterium</i>-Mediated Transformation of Mexican Lime (<i>Citrus aurantifolia</i> Swingle) Using Optimized Systems for Epicotyls and Cotyledons

Oliveira¹,

Moore²,

Thomson³

et al. 2015

ABB

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Transgenic Mexican lime (Citrus aurantifolia Swingle) was produced through two explant sources, each using systems previously optimized for each source. One used epicotyls segments, which was the predominant explant for transgenic Citrus production following co-cultivation with Agrobacterium, and has a well-established protocol. The other procedure used embryo cotyledons from mature seeds, which was developed in our lab as an alternative for stable Citrus transformation. Cotyledon transformation and regeneration protocols were optimized by comparing variables in culture medium composition on shoot regeneration and four parameters in transient transformation. The optimized protocols were compared, and frequency of regeneration, frequency of transgenic plant-recovery and stable transformation efficiency indicated the superiority of the cotyledon protocol for Agrobacterium-mediated genetic transformation in Mexican lime. The tissue choice resulted in marked improvement in shoot regeneration (14.1% of explants producing shoots in epicotyls; 55.8% in cotyledons), stable transformation frequency (11.4% of epicotyls explants; 40.2% in cotyledons), and frequency of transgenic plant-recovery (37.9% in epicotyl explants; 92.6% in cotyledons). Thus, easy availability of explants using embryo cotyledons from mature seeds, technical simplicity, shortening of transformation time-course, and higher transformation and regeneration frequencies makes this new system an attractive alternative over the previously published Citrus transformation protocols. In the course of this project, we generated Mexican lime with a Recombinase Mediated Exchange Cassette landing pad, which was designed for stacking transgenes.

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An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution

Cited by 179 publications

References 5 publications

Plant resistance to mechanical stress: evidence of an avoidance–tolerance trade‐off

Plant resistance to mechanical stress: evidence of an avoidance–tolerance trade‐off

Comparison of protocols for genomic DNA extraction from velame pimenta (Croton linearifolius), a native species to the Caatinga, Brazil

<i>Agrobacterium</i>-Mediated Transformation of Mexican Lime (<i>Citrus aurantifolia</i> Swingle) Using Optimized Systems for Epicotyls and Cotyledons

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An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution

Cited by 179 publications

References 5 publications

Plant resistance to mechanical stress: evidence of an avoidance–tolerance trade‐off

Plant resistance to mechanical stress: evidence of an avoidance–tolerance trade‐off

Comparison of protocols for genomic DNA extraction from velame pimenta (Croton linearifolius), a native species to the Caatinga, Brazil

&lt;i&gt;Agrobacterium&lt;/i&gt;-Mediated Transformation of Mexican Lime (&lt;i&gt;Citrus aurantifolia&lt;/i&gt; Swingle) Using Optimized Systems for Epicotyls and Cotyledons

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<i>Agrobacterium</i>-Mediated Transformation of Mexican Lime (<i>Citrus aurantifolia</i> Swingle) Using Optimized Systems for Epicotyls and Cotyledons