“…Hydroxyl number, the milligrams of potassium hydroxide equivalent to the hydroxyl content per 1 gram of sample, was determined using the pyridine-acetic anhydride method with minor modifications (74, 27,22,30). This consisted of acetylating 1 gram of the sample containing the hydroxyl compound, with a definite amount of a 25% mixture of acetic anhydride in pyridine by heating to 100°C.…”