An Improved Method for Propagation of Seabuckthorn (Hippophae rhamnoides L.) by Cuttings

Abstract

One year old growth stem cutting (2.9 ± 0.8 mm thickness), which is less than half of the conventional pencil thickness (7-8 mm) hardwood cutting, was studied for vegetative propagation of seabuckthorn (SBT). Rooting trial tested the effects of 14 media and 6 concentration of indole-3-butyric acid treatment. Over 94 % rooting was observed in all the media tested. The mean rooting percentage was 97.6 ± 2.2. Exogenous auxin treatments lack any benefit and rooting was over 97 % without hormonal treatment. Sevenfold more number of cuttings was obtained per plant as compared to pencil thickness hardwood cuttings, which is the conventional method of SBT propagation. The result of the present study could facilitate establishment of a vegetative propagation method wherein larger number of cuttings per plant can be propagated with higher rooting success rate without the need of exogenous application of rooting hormone.

A review on genetic diversity, micropropagation and transformations in the high-value medicinal plant of Himalayas-Seabuckthorn (Hippophae sp.)

“…Dale and Galic reported that prechilling of softwood cuttings for 6-8 weeks resulted in dormancy breaking with rooting up to 80% under greenhouse conditions [15] . The hardwood cuttings with less thickness (2.9 mm) showed a sevenfold increase with shooting and rooting in the absence of exogenous hormones [16] . Vegetative propagation from root cuttings and suckers is genetically identical (clones) to the parent plant, and these propagated plants may flower/fruit sooner than seed-propagated plants [13] .…”

Seabuckthorn (Hippophae rhamnoides L.) in trans-Himalayan Ladakh, India

“…First, propagation by a pencil thick hardwood cutting results in fewer cuttings per plant; secondly the rooting success rate is low. Therefore, Dolkar 14 , et al developed an improved method using previous season's growth stem cutting (2.9±0.8 mm thickness) for propagation of SBT. The improved method resulted in much higher rooting percentage than those reported for conventional method using a pencil thick hardwood cutting.…”