2016
DOI: 10.1038/srep26775
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An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples

Abstract: To explore the natural microbial community of any ecosystems by high-resolution molecular approaches including next generation sequencing, it is extremely important to develop a sensitive and reproducible DNA extraction method that facilitate isolation of microbial DNA of sufficient purity and quantity from culturable and uncultured microbial species living in that environment. Proper lysis of heterogeneous community microbial cells without damaging their genomes is a major challenge. In this study, we have de… Show more

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Cited by 168 publications
(120 citation statements)
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References 17 publications
(19 reference statements)
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“…Additionally, NGS methods have been refined to extract the microbial DNA of sufficient purity and quantity for such assays. 28 The increasing interest in NGS-based microbiome research has urged scientists to assess PTB as a polymicrobial disease, because the infectious agents could be bacteria, virus, or fungus such as yeast. the upper part of the myometrium.…”
mentioning
confidence: 99%
“…Additionally, NGS methods have been refined to extract the microbial DNA of sufficient purity and quantity for such assays. 28 The increasing interest in NGS-based microbiome research has urged scientists to assess PTB as a polymicrobial disease, because the infectious agents could be bacteria, virus, or fungus such as yeast. the upper part of the myometrium.…”
mentioning
confidence: 99%
“…Nevertheless, Henriques et al successfully purified freshwater samples on 5 ”m membranes, then concentrated microorganisms on 0.2 ”m pore-size filters, washed them in TE buffer, centrifuged (15 200 g, 10 min) and further processed by two commercial kits [42]. In a need for adequate DNA extraction from environmental samples, Bag et al proposed THSTI method using enzymes, buffers, glass and zirconia beads, among others [52].…”
Section: Extraction Of Dna From Arb and Tap Water Samplesmentioning
confidence: 99%
“…The applied procedures may affect all the subsequently obtained results. In the case of molecular biology investigations, the most essential stage is the extraction of DNA [52,53].…”
Section: Extraction Of Dna From Arb and Tap Water Samplesmentioning
confidence: 99%
“…Nevertheless, DNA extraction continues to be a large source of experimental variability in 16S rRNA sequence analysis and the most time-consuming step in a high-throughput pipeline [6]. Although DNA extraction protocols have been published for low bacterial biomasses [10,11], they are hard to implement as part of a high-throughput pipeline. Further, those focused on low biomass can become saturated with high biomass samples leading to uneven sequence sampling bias.…”
mentioning
confidence: 99%