An improved method for detection of ganglionic latency in herpes simplex virus type-1 infected guinea pigs

Bubel, H. Curt; Wander, Arden H.; McDowell, Shana

doi:10.1016/0166-0934(86)90055-8

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…When the experiments were concluded, the rabbits were sacrificed, and some trigeminal ganglia were removed to be processed as described by Bubel et al (1986). Infectious virus was recovered from trigeminal ganglion of both placebo and GN treated rabbits (2 rabbits processed per group) but not from GN-11 treated animals (all the animals were processed), nor from the ACV treated group that survived without infection for 60 days (3 rabbits) ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

Antiviral activity of a D‐glucosamine derivative against herpetic ulcers (HSV type 2) in rabbit cornea

Martin

Vilas

Prieto

et al. 1989

Acta Ophthalmologica

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Although most herpetic ocular infections in adults are caused by herpesvirus hominis type 1, several cases of culture proved HSV‐2 ocular infection in adults have been described, with more severe and prolonged disease. In a screening for new antiherpetic compounds, we investigated the efficacy in vivo of a new compound, nitroderivative of D‐glucosaminhydrochloride (GN‐11) in comparison with D‐glucosaminhydrochloride (GN), Acyclovir (ACV) and placebo against herpetic keratitis of herpes simplex type 2 in 4times4 eyes from 4times4 rabbits, respectively. ACV and GN‐11 showed similar results. The treatment with GN‐11 retarded the appearance of herpetic lesions, which were small and diffuse in comparison with the placebo group. A total recovery was obtained on the 12th day of the treatment. In the ACV treated group, a minimal number of small lesions appeared, but the eyes recovered normality on the 7th day of treatment. The appearance of acute herpetic keratitis was prevented by GN‐11. Placebo and GN treated groups showed similar evolution, with lost vision and neurological involvement on the 7th day of infection.

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Section: Resultsmentioning

confidence: 99%

Antiviral activity of a D‐glucosamine derivative against herpetic ulcers (HSV type 2) in rabbit cornea

Martin

Vilas

Prieto

et al. 1989

Acta Ophthalmologica

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“…In our hands, the McIntyre strain resulted in a 58 percent latency rate. This deserepency is probably due to the method employed to detect latency, since the ganglion culture method used in this study is considerably more sensitive than the eoeultivation technique (1). The kinetics of the acute H SV-1 (Sh) infection of the trigeminai ganglion (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…However, the experimental variability of this procedure prompted us to develop an alternative method for latency detection (1). Briefly, trigeminal ganglia were carefully removed, leaving the body of the ganglion and surrounding membranes intact.…”

Section: Assay For Latent Ganglionic Infectionsmentioning

confidence: 99%

The pathogenesis of herpetic ocular disease in the Guinea pig

Wander

Bubel

McDowell

1987

Archives of Virology

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A detailed study of the pathogenesis of herpetic eye disease in the guinea pig was undertaken to further develop this animal model. Several well-known HSV-1 strains were tested for their ability to produce disease and cause acute and latent infections of the trigeminal ganglion: McKrae, KOS, McIntyre, RE, and Shealey. Two HSV-2 strains failed to cause eye infections. The Shealey strain [HSV-1 (Sh)] produced the most severe eye infections, characterized by epithelial and stromal disease, corneal vascularization and ulcerative blepharitis. Consequently, HSV-1 (Sh) was selected as the prototype strain for this study. The frequency and severity of HSV-1 (Sh) eye disease patterns was determined by a semi-quantitative rating scale, which permitted accurate monitoring of the temporal development of the disease patterns cited above. Virus shedding from infected eyes was also quantified. All of the HSV-1 strains tested established trigeminal ganglionic latency with varying frequency, although HSV-1 (Sh) latency approached 100 percent. The kinetics of acute ganglionic infection by HSV-1 (Sh) was determined, and peak virus titers occurred on the third day after corneal inoculation. This study emphasizes the usefulness of the Guinea pig model for investigations on the pathogenesis, prevention and treatment of herpetic eye infections.

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“…If monolayers degener ated, tissues were transferred to fresh monolayers. G an glia were assayed by cultivation in high glucose media in roller tubes [15] and supernatants assayed weekly for 8 weeks for HSV CPE in GPE monolayers.…”

Section: Virus Recoverymentioning

confidence: 99%

Recurrent Genital Infection in the Guinea Pig: Differences between Herpes Simplex Types 1 and 2

Landry¹,

Myerson

Bull

1992

Intervirology

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Recurrence rates of genital infections are significantly higher for heφes simplex virus (HSV) type 2 than HSV type 1. Reasons for this difference are not known. In this report, multiple strains of HSV-1 and HSV-2 were evaluated in the guinea-pig model. HSV-2 strains showed significantly higher genital lesion recurrence than HSV-1, including HSV-1 McKrae strain which is highly recurrent in ocular infections. HSV-2 strains were also associated with more frequent asymptomatic vaginal virus shedding. Further study showed that HSV-1 strains replicated as well as HSV-2 in both the genital tract and the nervous system during acute infection. In addition, no difference was detected between HSV-1 and HSV-2 in nervous system latency. Thus, a number of possible explanations for the observed difference in genital heφes recurrence rates were examined and excluded.

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An improved method for detection of ganglionic latency in herpes simplex virus type-1 infected guinea pigs

Cited by 5 publications

References 10 publications

Antiviral activity of a D‐glucosamine derivative against herpetic ulcers (HSV type 2) in rabbit cornea

Antiviral activity of a D‐glucosamine derivative against herpetic ulcers (HSV type 2) in rabbit cornea

The pathogenesis of herpetic ocular disease in the Guinea pig

Recurrent Genital Infection in the Guinea Pig: Differences between Herpes Simplex Types 1 and 2

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