An improved mass spectrometric method for identification and quantification of phenolic compounds in apple fruits

Simultaneous determination of main phenolic acids and flavonoids in tomato by micellar electrokinetic capillary electrophoresis

“…Recoveries were calculated in spiked tomato samples (average, n = 5) and ranged from 77 to 106% for the low fortification level (12.5 mg kg −1 ), and from 77 to 103% for the high level (50 mg kg −1 ). These results were comparable or even better than those reported by other authors (De Paepe et al, 2013;Ribas-Agustí et al, 2012;Sakakibara, Honda, Nakagawa, Ashida, & Kanazawa, 2003).…”

“…Compared to other methods employing similar CE techniques, these obtained %RSD values were similar or even lower (Arce, Ríos, & Valcárcel, 1998;Ehala et al, 2005;HerreroMartínez et al, 2007). Moreover, compared to HPLC alternatives the obtained %RSD values were similar to those reported by other authors using DAD detection (Ribas-Agustí, Cáceres, Gratacós-Cubarsí, Sárraga, & Castellari, 2012) or even lower than those reported in HPLC-MS studies (De Paepe et al, 2013). Intra-(n = 5) and inter-day (n = 2) precision of migration times ranged from <0.1 to 0.2% and from <0.1 to 1.4%, respectively, for standards, and from <0.1 to 0.2% and from 0.3 to 1.7% respectively for spiked samples (Table 3).…”

“…Intra-(n = 5) and inter-day (n = 2) precision of migration times ranged from <0.1 to 0.2% and from <0.1 to 1.4%, respectively, for standards, and from <0.1 to 0.2% and from 0.3 to 1.7% respectively for spiked samples (Table 3). These values are similar to those reported by other authors (De Paepe et al, 2013). Table 3 Analytical performance parameters of the optimized method using polyphenol standards and spiked tomato samples.…”

Variability of the phenolic profiles in the fruits from old, recent and new apple cultivars cultivated in Belgium

“…An ultrasound assisted consecutive extraction with MeOH/ H 2 O (20/80, v/v) and 100 % MeOH on 0.5 g freeze-dried material was carried out (De Paepe et al 2013). The liquid chromatographic (LC) system consisted of an Accela TM quaternary solvent manager, a 'Hot Pocket' column oven (Thermo Fisher Scientific, Bremen, Germany) and a CTC PAL TM autosampler (CTC Analytics, Zwingen, Switzerland).…”

Improved Characterization of Polyphenols Using Liquid Chromatography

“…De Paepe et al (2013) developed and validated a method to identify phenolic compounds in apple extracts using UHPLC coupled with an Orbitrap. An accurate mass spectrometry technique allowed the identification of 39 phenolic compounds in apples, including flavonoids, proanthocyanidins, and phenolic acids.…”