An improved hydrophilicity via electrospinning for enhanced cell attachment and proliferation

Kim, Chi Hun; Khil, Myung-Seob; Kim, Hee Young; Lee, Hyun Uk; Jahng, Kwang Yeop

doi:10.1002/jbm.b.30484

Cited by 276 publications

(154 citation statements)

References 57 publications

(88 reference statements)

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“…The electrospinning technique is an efficient method of producing continuous and fine (micro/nano) scale fibers by drawing out the polymer solution under electrostatic forces into the whipping jets, and continuous fiber can be deposited on the conductive collector (Kim et al 2006). Electrospinningprocessing parameters such as solutions, distances between tips to collector, high-voltage supply, flow rate of injection pump and room humidity can be optimized to obtain beadless and uniform nanofibers (Chung et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Hassan

Sultana

2017

3 Biotech

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Considering the important factor of bioactive nanohydoxyapatite (nHA) to enhance osteoconductivity or bone-bonding capacity, nHA was incorporated into an electrospun polycaprolactone (PCL) membrane using electrospinning techniques. The viscosity of the PCL and nHA/PCL with different concentrations of nHA was measured and the morphology of the electrospun membranes was compared using a field emission scanning electron microscopy. The water contact angle of the nanofiber determined the wettability of the membranes of different concentrations. The surface roughness of the electrospun nanofibers fabricated from pure PCL and nHA/PCL was determined and compared using atomic force microscopy. Attenuated total reflectance Fourier transform infrared spectroscopy was used to study the chemical bonding of the composite electrospun nanofibers. Beadless nanofibers were achieved after the incorporation of nHA with a diameter of 200-700 nm. Results showed that the fiber diameter and the surface roughness of electrospun nanofibers were significantly increased after the incorporation of nHA. In contrast, the water contact angle (132°± 3.5°) was reduced for PCL membrane after addition of 10% (w/ w) nHA (112°± 3.0°). Ultimate tensile strengths of PCL membrane and 10% (w/w) nHA/PCL membrane were 25.02 ± 2.3 and 18.5 ± 4.4 MPa. A model drug tetracycline hydrochloride was successfully loaded in the membrane and the membrane demonstrated good antibacterial effects against the growth of bacteria by showing inhibition zone for E. coli (2.53 ± 0.06 cm) and B. cereus (2.87 ± 0.06 cm).

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Section: Introductionmentioning

confidence: 99%

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Hassan

Sultana

2017

3 Biotech

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“…Some studies observed that PCL fibrous scaffolds caused a reduction in the ability of cell attachment, migration, proliferation, and differentiation [8]. Cell affinity towards synthetic polymer is usually poorer than natural polymer.…”

Section: -2506mentioning

confidence: 99%

Comparison on in Vitro Degradation of Polycaprolactone and Polycaprolactone/Gelatin Nanofibrous Scaffold

2017

MJAS

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Tissue engineering has emerged to provide a new medical therapy in helping tissue regrowth and regeneration by employing scaffold as an artificial supporting structure for cellular growth. Many polymers have been utilized in the fabrication of these artificial scaffolds, but there is still a need to fabricate hydrophilic nanofibrous scaffold with appropriate degradation rate. In this study, polycaprolactone (PCL) and polycaprolactone/gelatin (PCL/Ge) 70:30 nanofibrous scaffolds were fabricated using electrospinning technique and compared on in vitro degradation rate to determine a more suitable scaffold for skin tissue engineering application. In vitro degradation was evaluated by morphological changes, water uptake, and chemical bonding until 12 weeks. Result shows that both PCL and PCL/Ge (70:30) nanofibrous scaffolds were degraded after 8th week. However, the degradation rate of PCL nanofibrous scaffold is slower and does not has obvious morphological changes. PCL/Ge (70:30) nanofibrous scaffold with faster degradation rate have the potential for skin tissue engineering application.Keywords: tissue engineering, nanofibrous scaffold, in vitro degradation, polycaprolactone, polycaprolactone/gelatin Abstrak Kejuruteraan tisu muncul untuk menyediakan terapi perubatan baru dalam membantu pertumbuhan semula tisu dengan menggunakan perancah sebagai struktur sokongan tiruan untuk pertumbuhan selular. Banyak polimer telah digunakan dalam penghasilan perancah ini, namun masih wujud permintaan terhadap penghasilan perancah gentian hidrofilik yang bersaiz nano dengan kadar degradasi yang sesuai. Dalam kajian ini, gentian perancah nano polikaprolakton (PCL) dan polikaprolakton/gelatin (PCL/Ge) 70:30 telah dihasilkan dengan teknik putaran elekron dan kadar degradasi in vitro dibandingkan untuk menentukan perancah yang lebih sesuai bagi aplikasi kejuruteraan tisu kulit. Degradasi in vitro telah dinilai melalui perubahan morfologi, penyerapan air, dan ikatan kimia kedua-dua gentian perancah nano sehingga 12 minggu. Keputusan menunjukkan kedua-dua PCL dan PCL/Ge (70:30) gentian perancah nano telah degradasi selepas minggu ke-8. Walau bagaimanapun, kadar degradasi gentian perancah nano PCL adalah lebih perlahan dan tiada perubahan morfologi yang jelas. Gentian perancah nano PCL/Ge (70:30) dengan kadar degradasi yang lebih cepat mempunyai potensi dalam bidang kejuruteraan tisu kulit.Kata kunci: kejuruteraan tisu, perancah gentian nano, degradasi in vitro, polikaprolakton, polikaprolakton/gelatin Introduction Tissue engineering is an interdisciplinary field that uses life sciences and engineering principles to develop biological substitutes for example fibrous scaffold for improving, maintaining and restoring the function of tissues

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“…The reason for using different biopolymers is that PCL is a biodegradable aliphatic polyester with a high strength, which is needed during artificial blood vessel implantation [11][12][13][14]. Even though electrospun PCL mats mimic the extracellular matrix in living tissues, their poor hydrophilicity decreases cell adhesion, migration, proliferation and differentiation [15]. In contrast, gelatin is a natural biopolymer derived from collagen through controlled hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

The effect of cross-linking on the microstructure, mechanical properties and biocompatibility of electrospun polycaprolactone–gelatin/PLGA–gelatin/PLGA–chitosan hybrid composite

Nguyen

Lee

2012

Science and Technology of Advanced Materials

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In this study, multilayered scaffolds composed of polycaprolactone (PCL)-gelatin/poly(lactic-co-glycolic acid) (PLGA)-gelatin/PLGA-chitosan artificial blood vessels were fabricated using a double-ejection electrospinning system. The mixed fibers from individual materials were observed by scanning electron microscopy. The effects of the cross-linking process on the microstructure, mechanical properties and biocompatibility of the fibers were examined. The tensile stress and liquid strength of the cross-linked artificial blood vessels were 2.3 MPa and 340 mmHg, respectively, and were significantly higher than for the non-cross-linked vessel (2.0 MPa and 120 mmHg). The biocompatibility of the cross-linked artificial blood vessel scaffold was examined using the MTT assay and by evaluating cell attachment and cell proliferation. The cross-linked PCL-gelatin/PLGA-gelatin/ PLGA-chitosan artificial blood vessel scaffold displayed excellent flexibility, was able to withstand high pressures and promoted cell growth; thus, this novel material holds great promise for eventual use in artificial blood vessels.

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An improved hydrophilicity via electrospinning for enhanced cell attachment and proliferation

Cited by 276 publications

References 57 publications

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

Comparison on in Vitro Degradation of Polycaprolactone and Polycaprolactone/Gelatin Nanofibrous Scaffold

The effect of cross-linking on the microstructure, mechanical properties and biocompatibility of electrospun polycaprolactone–gelatin/PLGA–gelatin/PLGA–chitosan hybrid composite

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