An Improved Automated Determination of Serum Total Cholesterol with a Single Color Reagent

Abstract

An anhydrous color reagent containing ferric chloride is pumped through Tygon tubing to a glass coil in a 95° heating bath. Manually prepared serum extracts (1:20 isopropanol dilution) are presented to the stream of preheated color reagent, and the two are passed through three mixing coils, connected in series. The absorbance of the resultant color is determined at 550 mµ in a 15 mm. tubular flow-cell. The improved N-automated procedure gave values of greater precision (5.6%) for total cholesterol determined in replicate samples from sera pools than the N-automated procedure which lacked precision (27.7%). The values found in 60 individual serums are within 6.0% of total cholesterol values determined by the Abell et al. method.

Effects of Age, Physical Training, and Physical Fitness on Coronary Heart Disease Risk Factors in Older Track Athletes at Twenty‐Year Follow‐up

“…A positive trend (decline) in the change in prevalence of elevated total cholesterol levels among subjects was observed between T1 and T3 (Table 3). At T1, mean total cholesterol levels for all subjects and group H were above current recommendations (<200 mg/dL) 28 (Table 2). Total cholesterol levels declined significantly at T2 for all subjects (−13%) and group H (−19%) and were within risk factor guidelines.…”

“…Serum total cholesterol assays were done at different lipid research laboratories. However, cholesterol measurements from each evaluation were standardized to criteria of the Centers for Disease Control and Prevention lipid laboratory program, 26 which are based on the reference cholesterol method of Abell et al 27 At T1, total cholesterol was determined using the automated analytic procedure of Block et al 28 At T2 and T3, total cholesterol was analyzed enzymatically based on the procedures of Allain et al 29 Classifications for elevated total cholesterol levels followed recommendations given by the Expert Panel on the Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults 30…”

Lp(a) lipoprotein enters cultured fibroblasts independently of the plasma membrane low density lipoprotein receptor

“…Lipid determinations. Serum total cholesterol and triglyceride determinations were carried out in an AutoAnalyzer by the methods of Kessler & Lederer (1965) and Block et al (1966), respectively. Serum fractionation and lipoprotein radiolabelling.…”

Some effects of fats with dietary carbohydrates on the lipids on the surface of the skin

“…The lipid solvent was filtered through fat-free shark skin filter paper and then evaporated to dryness. The lipid was re-dissolved and the cholesterol concentration measured on an aliquot by the method of Block, Jarrett & Levine (1966). The triglyceride concentration was determined by an automated method based on that described by Lofland (1964).…”