An improved analytical method for determination of trans‐resveratrol and related stilbenes in grape skin by QuEChERS coupled with HPLC‐PDA‐MS

Abstract

Grape is a popular fruit crop that generates about one-fourth of total weight as pomace during winemaking and commercial juice production. However, a significant amount of resveratrol and related stilbenes is present in grape skin, and their effective isolation enables the production of functional foods. Herein, an improved analytical method for the determination of trans-resveratrol and related stilbenes in grape skin waste by QuEChERS method coupled with a high-performance liquid chromatograph-photodiode array detector-mass spectrometer was developed. By employing a Gemini C18 column and a mobile phase of acetic acid in water (pH 3.6) and acetonitrile, a total of five resveratrol and related stilbenes could be separated within 15 min with a flow rate of 0.8 mL min −1 and detection wavelength at 306 nm. The optimised QuEChERS conditions were 10 mL of acetonitrile, 1 mL of grape skin extract and 900 mg of magnesium sulphate plus 25 mg of primary secondary amine. Principal component analysis by two principal components (74.18%, 25.82%) could well describe the trans-resveratrol recovery at different QuE-ChERS conditions. A mean recovery, as well as coefficient of variation (CV, %) of repeatability and intermediate precision, ranged from 96.6% to 104.4%, 1.96% to 3.10% and 1.68% to 4.27%, respectively, conforming to the regulation set by Taiwan Food and Drug Administration. Quantitation revealed cispiceid to be present in largest amount (2.616 μg mL −1 ), followed by trans-piceid (1.027 μg mL −1 ), transresveratrol (0.967 μg mL −1 ), ε-viniferin (0.660 μg mL −1 ) and cis-resveratrol (0.183 μg mL −1 ) in grape skin extract, with their corresponding contents in dried grape skin being 4.185, 1.643, 1.539, 1.056 and 0.293 μg g −1 , respectively.

Comparative Study on Inhibition of Pancreatic Cancer Cells by Resveratrol Gold Nanoparticles and a Resveratrol Nanoemulsion Prepared from Grape Skin

“…This procedure was repeated 3 times and all the organic layers were pooled in a separatory funnel, followed by adding 5% sodium bicarbonate solution (pH 8-9) at a volume ratio of 1:1, washing until the organic layer became colorless, evaporating to dryness, dissolving in 0.5 mL of methanol, and filtering through a 0.22 µm membrane filter for HPLC analysis. Trans-resveratrol and cis-resveratrol in the grape skin were separated, identified, and quantified using a method described in a previous study [13].…”

“…Then, both the size and shape of the resveratrol nanoemulsion were obtained. Also, the encapsulation efficiency was determined by collecting a portion (100 µL) of the resveratrol nanoemulsion and diluting it with deionized water 10 times, followed by pouring into a centrifuge tube containing a 3 kDa dialysis membrane, centrifuging at 12,000× g for 20 min (25 • C), collecting the lower layer (200 µL) containing free resveratrol, evaporating to dryness, and dissolving in methanol for HPLC analysis of resveratrol [13]. Then, the encapsulation efficiency of resveratrol was obtained by using a formula described in a previous study [14].…”

“…Based on the extraction and HPLC method described in a previous study [13], a total of five trans-resveratrol and related stilbenes in grape skin were identified and quantified, with cis-piceid present in the largest amount (4.185 µg/g), followed by trans-piceid (1.643 µg/g), trans-resveratrol (1.539 µg/g), ε-viniferin (1.056 µg/g), and cis-resveratrol (0.293 µg/g).…”

The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS

“…Several research studies have associated EA with beneficial pharmacological activities, above all against in diabetes, cancer, neurodegenerative disorders and cardiovascular diseases, and it has attracted great interest recently [13][14]. Many research studies have demonstrated the many biological functions of RES, including antioxidant, antiinflammation, anti-cancer, anti-diabetes, anti-obesity, anti-microbial, anti-aging, cardio protection, and neuroprotection [15].…”