An impedance-based high-throughput method for evaluating the cytotoxicity of nanoparticles

Cimpan, Mihaela Roxana; Mordal, T; Schölermann, Julia; Allouni, Zouhir Ekeland; Pliquett, Uwe; Cimpan, Emil

doi:10.1088/1742-6596/429/1/012026

Cited by 14 publications

(34 citation statements)

References 23 publications

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“…Impedance‐based sensing provides information about the properties of the cells as a function of electric signal frequency . Several cellular behaviors can be assessed using impedance‐based assays, for example cell adhesion, spread, viability, and proliferation . Our findings show that nano‐TiO 2 had no effect on the viability of SaOS‐2 within 24 h of exposure and the cells showed increased proliferation in response to Ti5.…”

Section: Discussionmentioning

confidence: 76%

“…Cell viability and proliferation were collectively assessed in real‐time using the label‐free impedance‐based xCELLigence system (ACEA Biosciences, CA). Impedance‐based sensing methods are used to gain information about various cell properties, such as proliferation, growth, morphology, and intercellular adhesion as functions of the applied electrical signal frequency . The xCELLigence system monitors over a desired period of time the impedance of the cells seeded on top of gold‐plated electrodes.…”

Section: Methodsmentioning

confidence: 99%

“…A change in CI can be indicative of changes in cell number, cell adhesion, spreading, or cell morphology. The CI goes up when the cell number increases, flattens at full confluency, and decreases when cells die . For this experiment, SaOS‐2 cells were seeded in 16‐well e‐plates with gold‐plated electrodes and were allowed to attach and proliferate for 24 h after which they were exposed to the nano‐TiO 2 for 24 h. Untreated cells served as controls.…”

Section: Methodsmentioning

confidence: 99%

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Ibrahim

Schoelermann

Mustafa

et al. 2018

J Biomedical Materials Res

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Human exposure to titanium dioxide nanoparticles (nano-TiO ) is increasing. An internal source of nano-TiO is represented by titanium-based orthopedic and dental implants can release nanoparticles (NPs) upon abrasion. Little is known about how the size of NPs influences their interaction with cytoskeletal protein networks and the functional/homeostatic consequences that might follow at the implant-bone interface with regard to osteoblasts. We investigated the effects of size of anatase nano-TiO on SaOS-2 human osteoblast-like cells exposed to clinically relevant concentrations (0.05, 0.5, 5 mg/L) of 5 and 40 nm spherical nano-TiO . Cell viability and proliferation, adhesion, spread and migration were assessed, as well as the orientation of actin and microtubule cytoskeletal networks. The phosphorylation of focal adhesion kinase (p-FAK ) and the expression of vinculin in response to nano-TiO were also assessed. Treatment with nano-TiO disrupted the actin and microtubule cytoskeletal networks leading to morphological modifications of SaOS-2 cells. The phosphorylation of p-FAK and the expression of vinculin were also modified depending on the particle size, which affected cell adhesion. Consequently, the cell migration was significantly impaired in the 5 nm-exposed cells compared to unexposed cells. The present work shows that the orientation of cytoskeletal networks and the focal adhesion proteins and subsequently the adhesion, spread and migration of SaOS-2 cells were affected by the selected nano-TiO in a size dependent manner. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2582-2593, 2018.

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Section: Discussionmentioning

confidence: 76%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Ibrahim

Schoelermann

Mustafa

et al. 2018

J Biomedical Materials Res

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“…The differences in the nature of the two cell lines, that is, adherent vs. suspended, necessitated the utilization of two different impedance‐based instruments. Both instruments are described in more detail elsewhere …”

Section: Methodsmentioning

confidence: 99%

In vitro cytotoxicity assessment of nanodiamond particles and their osteogenic potential

Ibrahim

Xue

Ostermann

et al. 2018

J Biomedical Materials Res

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Scaffolds functionalized with nanodiamond particles (nDP) hold great promise with regard to bone tissue formation in animal models. Degradation of the scaffolds over time may leave nDP within the tissues, raising concerns about possible long-term unwanted effects. Human SaOS-2 osteoblast-like cells and U937 monoblastoid cells were exposed to five different concentrations (0.002-2 mg/L) of nDP (size range: 2.36-4.42 nm) for 24 h. Cell viability was assessed by impedance-based methods. The differential expression of stress and toxicity-related genes was evaluated by polymerase chain reaction (PCR) super-array, while the expression of selected inflammatory and cell death markers was determined by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Furthermore, the expression of osteogenic genes by SaOS-2 cells, alkaline phosphatase activity and the extracellular calcium nodule deposition in response to nDP were determined in vitro. Cells responded differently to higher nDP concentrations (≥0.02 mg/L), that is, no loss of viability for SaOS-2 cells and significantly reduced viability for U937 cells. Gene expression showed significant upregulation of several cell death and inflammatory markers, among other toxicity reporter genes, indicating inflammatory and cytotoxic responses in U937 cells. Nanodiamond particles improved the osteogenicity of osteoblast-like cells with no evident cytotoxicity. However, concentration-dependent cytotoxic and inflammatory responses were seen in the U937 cells, negatively affecting osteogenicity in co-cultures. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1697-1707, 2018.

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“…For monitoring changes in proliferation and viability of cells, impedance measurements were performed with the xCelligence System (ACEA Biosciences Inc, San Diego, CA) (Cimpan et al, 2013). After washing, DiD-stained cells were placed on electrodes at a density of 11 000 cells/cm 2 .…”

Section: Impedance Measurementsmentioning

confidence: 99%

Contact-dependent transfer of TiO₂nanoparticles between mammalian cells

et al. 2015

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Cellular organelles have been shown to shuttle between cells in co-culture. We hereby show that titanium dioxide (TiO2) nanoparticles (NPs) can be transferred in such a manner, between cells in direct contact, along with endosomes and lysosomes. A co-culture system was employed for this purpose and the NP transfer was observed in mammalian cells including normal rat kidney (NRK) and HeLa cells. We found that the small GTPase Arf6 facilitates the intercellular transfer of smaller NPs and agglomerates. Spherical, anatase nano-TiO2 with sizes of 5 (Ti5) and 40 nm (Ti40) were used in this study. Humans are increasingly exposed to TiO2 NPs from external sources such as constituents of foods, cosmetics, and pharmaceuticals, or from internal sources represented by Ti-based implants, which release NPs upon abrasion. Exposure to 5 mg/l of Ti5 and Ti40 for 24 h did not affect cellular viability but modified their ability to communicate with surrounding cells. Altogether, our results have important implications for the design of nanomedicines, drug delivery and toxicity.

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An impedance-based high-throughput method for evaluating the cytotoxicity of nanoparticles

Cited by 14 publications

References 23 publications

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

In vitro cytotoxicity assessment of nanodiamond particles and their osteogenic potential

Contact-dependent transfer of TiO₂nanoparticles between mammalian cells

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An impedance-based high-throughput method for evaluating the cytotoxicity of nanoparticles

Cited by 14 publications

References 23 publications

TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

TiO2 nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

In vitro cytotoxicity assessment of nanodiamond particles and their osteogenic potential

Contact-dependent transfer of TiO2nanoparticles between mammalian cells

Contact Info

Product

Resources

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TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

TiO₂ nanoparticles disrupt cell adhesion and the architecture of cytoskeletal networks of human osteoblast‐like cells in a size dependent manner

Contact-dependent transfer of TiO₂nanoparticles between mammalian cells