An immunohistological study of secondary Sjogren's syndrome.

Isenberg, David; Rowe, Dorothy J.; Tookman, AG; Hopp, Ann-Katrin; Griffiths, Meryl; Paice, E. W.; Stewart, Jane E.; Beverley, Peter C. L.

doi:10.1136/ard.43.3.470

Cited by 52 publications

(17 citation statements)

References 15 publications

(12 reference statements)

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“…The infiltrate is composed of T and B lymphocytes, plasma cells and a small number of macrophages [1,2]. Most of the T cells are CD4 [3][4][5] bearing the phenotype of memory cells [6,7] with 15-20% being CD8 cells [3,4]. A large proportion of the T cells express class II MHC antigens [8][9][10], suggesting activation, though few (4%) express the T cell activation antigen.…”

Section: Introductionmentioning

confidence: 99%

T cell clones from a Sjögren’s syndrome salivary gland biopsy produce high levels of IL-10

Brookes

Cohen

Price

et al. 1996

Clinical and Experimental Immunology

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SUMMARYSjögren's syndrome (SS) is characterized by a focal periductal salivary gland infiltrate consisting mainly of T and B lymphocytes. Most of the T cells bear the memory of CD4 Th-1-like phenotype and express high levels of class II, though CD8 cells are also present. We have studied 17 labial salivary gland and 15 peripheral blood T cell clones from a patient with primary SS. The tissue clones were 71% CD8 and 29% CD4 , and the peripheral blood-derived clones were 60% CD8 and 40% CD4 . The CD4 T cell clones from both the salivary gland and autologous peripheral blood were of the Th1 phenotype, in that they produced interferon-gamma (IFN-) and IL-2 but very little IL-4 after 24 h stimulation with phorbol myristate acetate and anti-CD3 antibody. The salivary gland-derived CD4 clones produced 15 times more IL-10 (7 . 92 ng/ml) than peripheral blood-derived CD4 clones (0 . 52 ng/ ml, P

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Section: Introductionmentioning

confidence: 99%

T cell clones from a Sjögren’s syndrome salivary gland biopsy produce high levels of IL-10

Brookes

Cohen

Price

et al. 1996

Clinical and Experimental Immunology

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“…In addition, a number of serum samples from idiotype-positive patients were studied when the disease was inactive and serial bleeds were assessed (see Results). The sera from 27 patients with rheumatoid arthritis (each ofwhom met the revised criteria of the American Rheumatism Association [12]) (mean age = 57±13.4; F/M = 14:13), 20 patients with primary Sjbgren's syndrome (who fulfilled criteria for the disease [13]) (mean age = 58±14.5; F/M = 15:5), 17 patients with idiopathic myositis (14) (mean age = 46±14.5; F:M 12:5), and 28 normal controls (mean age = 39±1 1; F/M = 19:9) were also examined.…”

Section: Introductionmentioning

confidence: 99%

Serological and genetic characterization of a human monoclonal immunoglobulin G anti-DNA idiotype.

Ehrenstein

Longhurst²,

Latchman³

et al. 1994

J. Clin. Invest.

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This study analyzed the distribution of an idiotype, B3-Id, in patients with active SLE, classified according to organ involvement, normal controls, and other autoimmune rheumatic diseases. A polyclonal anti-idiotype was raised by immunizing a rabbit with a monoclonal IgG anti-double-stranded (ds) DNA antibody, B3, generated from a patient with SLE who had active arthritis. The idiotype is present on the X chain and is at or near the binding site for double-stranded DNA. The X chain, which was characterized by nucleotide sequencing, was 90% homologous to the VX2.1 germline, which is known to be involved in coding for nephritogenic anti-DNA antibodies carrying the 8.12 idiotype. There were four changes to positively charged amino acids, known to be involved in DNA binding, in the complementarity determining regions of B3 X chain compared with a non-DNA binding, 8.12 positive antibody, PVll. Only one change to a positively charged amino acid occurs in the heavy chain of B3, which is 93.5% homologous to VH-26.The B3-Id was present on IgG antibodies in the serum of 20% of patients with SLE but was not found in the normal controls. Within the SLE group, there is a statistically significant association of B3-Id on IgG in the arthritis group (42%) compared to the other manifestations (9%) (P < 0.001). In four B3-Id-positive SLE patients tested serially, the level of B3-Id reflected the arthritis disease activity more closely than the overall disease activity (P < 0.05). The B3-Id was also present on IgM antibodies in one third of patients with rheumatoid arthritis.This idiotype is the first to be derived from a human monoclonal anti-DNA antibody of the IgG class, the isotype associated with active disease. Sequence analysis shows that positively charged amino acids on the X chain may contribute to DNA binding. (J. Clin. Invest. 1994. 93:1787-1797

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“…Sjogren's syndrome (SS) is a chronic autoimmune disease characterized by the clinical manifestations of xerostomia and keratoconjunctivitis sicca (KCS) related to lymphocytic infiltration and dysfunction of the salivary and lacrimal glands (1)(2)(3)(4)(5)(6)(7). SS has been divided into primary and secondary forms, dependent on the presence of another autoimmune or connective tissue disease (7).…”

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confidence: 99%

“…SS has been divided into primary and secondary forms, dependent on the presence of another autoimmune or connective tissue disease (7). Currently, the diagnosis of SS is defined by the presence of KCS, as determined by complete ophthalmologic evaluation, and a positive sublabial salivary gland (SSG) biopsy result (5,8,9). Focal sialadenitis of grade IV (lo), which corresponds to a lymphocytic focus score > 1 focusl4 mm2 of SSG tissue, is a widely accepted criterion for the histologic confirmation of the diagnosis of SS (1)(2)(3)(4)(5)(6)(7)(8)(9).…”

mentioning

confidence: 99%

“…Currently, the diagnosis of SS is defined by the presence of KCS, as determined by complete ophthalmologic evaluation, and a positive sublabial salivary gland (SSG) biopsy result (5,8,9). Focal sialadenitis of grade IV (lo), which corresponds to a lymphocytic focus score > 1 focusl4 mm2 of SSG tissue, is a widely accepted criterion for the histologic confirmation of the diagnosis of SS (1)(2)(3)(4)(5)(6)(7)(8)(9). However, this criterion results in 6 9 % falsepositive diagnoses in a normal population (1 1,12), and 1840% of patients with clinically diagnosed SS have a lymphocytic focus score of 11 (9,10,(13)(14)(15).…”

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confidence: 99%

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A new and highly sensitive immunohistologic diagnostic criterion for Sjögren's syndrome

Wilde

Kater

Baak

et al. 1989

Arthritis & Rheumatism

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Immunohistologic investigation of the sublabial salivary glands of 65 surgical patients who were free of systemic disease (control) and 19 patients with Sjogren's syndrome resulted in a new diagnostic criterion for SS that was more disease-specific than the widely used lymphocytic focus wore criterion. Our bivariate classification criterion, which is based on the percentages of IgA-and IgG-containing plasma cells, has a specificity of 95.4% and a sensitivity of loo%, while its overall percentage of misclassification is 3.6%. This immunohistologic diagnostic criterion results in a reduction of the percentage of false-positive diagnoses from 9% to 4.6%.Sjogren's syndrome (SS) is a chronic autoimmune disease characterized by the clinical manifestations of xerostomia and keratoconjunctivitis sicca (KCS) related to lymphocytic infiltration and dysfunction of the salivary and lacrimal glands (1-7). SS has

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An immunohistological study of secondary Sjogren's syndrome.

Cited by 52 publications

References 15 publications

T cell clones from a Sjögren’s syndrome salivary gland biopsy produce high levels of IL-10

T cell clones from a Sjögren’s syndrome salivary gland biopsy produce high levels of IL-10

Serological and genetic characterization of a human monoclonal immunoglobulin G anti-DNA idiotype.

A new and highly sensitive immunohistologic diagnostic criterion for Sjögren's syndrome

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