An immunohistochemical study of the localization of biglycan, decorin and large chondroitin-sulphate proteoglycan in adult rat temporomandibular joint disc

Mizoguchi, Itaru; Scott, Paul; Dodd, Carole M.; Rahemtulla, Firoz; Sasano, Yasuyuki; Kuwabara, Mikio; Satoh, Seiji; Saitoh, S.; Hatakeyama, Yuji; Kagayama, Manabu; Mitani, Hideo

doi:10.1016/s0003-9969(98)00038-7

Cited by 37 publications

(47 citation statements)

References 35 publications

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“…In our group, the porcine disc was determined to be stiffer in the posterior band than in the anterior band and in the center of the intermediate zone (Allen and Athanasiou, in press;Kim et al, 2003). In contrast, a study of human TMJ discs found the discs to be stiffer under compression in the intermediate zone than in the anterior and posterior bands (Beek et al, 2001), which has been supported by finite element studies of the human TMJ (Beek et al, 2000;Mizoguchi et al, 1998).…”

Section: Introductionmentioning

confidence: 51%

“…The center of the bovine disc was determined to contain higher amounts of chondroitin sulfate, dermatan sulfate, keratan sulfate, and hyaluronic acid than the periphery. In contrast, large chondroitin sulfate proteoglycans in the rat disc were more concentrated in the periphery compared to the center, and dermatan sulfate proteoglycans were more prevalent in the anterior band and posterior band compared to the intermediate zone (Kuwabara et al, 2002;Mizoguchi et al, 1998).…”

Section: Introductionmentioning

confidence: 71%

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Quantitative analysis and comparative regional investigation of the extracellular matrix of the porcine temporomandibular joint disc

et al. 2005

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Characterization of the extracellular matrix of the temporomandibular joint (TMJ) disc is crucial to advancing efforts in tissue engineering the disc. However, the current literature is incomplete and often contradictory in its attempts to describe the nature of the TMJ disc matrix. The aim of this study was to identify the variation of key matrix components along the three axes of the porcine disc using ELISAs to quantify these matrix components, immunohistochemistry to identify their regional distribution, and SEM to examine collagen fiber diameter and orientation. The overall GAG content of the TMJ disc (including the dermatan sulfate proteoglycans) was 5.3+/-1.2% of the dry weight. Chondroitin sulfate, which comprised 74% of this total GAG content, was 4.4, 8.2, and 164 times more abundant than dermatan sulfate proteoglycan, keratan sulfate, and hyaluronic acid, respectively. In general, these GAGs were most concentrated in the intermediate zone of the TMJ disc, appearing in dense clusters, and least concentrated in the posterior band. Additionally, chondroitin sulfate was more abundant medially than laterally. Collagen II was discovered in trace amounts, with higher relative amounts in the intermediate zone. Collagen fibers were observed to run primarily in a ring-like fashion around the periphery of the disc and anteroposteriorly through the intermediate zone, with a mean fiber diameter of 18+/-9 mum. Characterization studies of the TMJ disc, including prior biomechanical and cell studies along with the current study of the extracellular matrix, collectively reveal a distinct character of the intermediate zone of the disc compared to its anterior and posterior bands.

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Section: Introductionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 71%

Quantitative analysis and comparative regional investigation of the extracellular matrix of the porcine temporomandibular joint disc

et al. 2005

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“…They also discussed the disk wear in the anterior [26] and intermediate [46] regions. As mentioned in the preceding sections in this article, these phenomena have already been explained in association with excessive loading validated by finite element stress analyses [18][19][20]23] and histological or histochemical examination which revealed stress concentration by the presence of chondroitin sulfate [47,48]. In addition, it was demonstrated in a previous study [21] that stresses in the retrodiscal tissues were increased by anterior disk displacement and may lead to the thinning and perforation, indicating an important role of the disk as a stress absorber.…”

Section: Role Of Articular Disk In Buffering or Absorbing Stresses Onmentioning

confidence: 57%

Degenerative changes of articular cartilage in association with mechanical stimuli

Tanne

2008

Japanese Dental Science Review

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“…In this respect, it has already been shown that the mAb 5D5 does not recognize cartilagespecific aggrecan (Bratt et al 1992;Mizoguchi et al 1998). On the other hand, Western blot analysis using brain PGs indicated that the mAb 5D5 cross-reacted with four brain-related PGs other than versican.…”

Section: Discussionmentioning

confidence: 97%

“…Histological and chemical analyses of the bovine TMJ discs showed the regional differences in ECM organization and composition; the peripheral region had (1) thinner and more loosely packed collagen fibrils, (2) less chondroitin sulphate, (3) more decorin, and (4) less content of biglycan, compared to those of the peripheral region (Nakano and Scott 1989;Kuc and Scott 1994;Scott et al 1995;Mizoguchi et al 1998). These regional differences are thought, at least in part, to reflect the biomechanical environment of the TMJ disc.…”

Section: Discussionmentioning

confidence: 99%

Expression and localization of versican during postnatal development of rat temporomandibular joint disc

Toriya

Teratani

Arakawa

et al. 2005

Histochem Cell Biol

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To analyze the growth-related changes in extracellular matrix components in temporomandibular joint (TMJ) discs, the expression and localization of the core protein of a large chondroitin sulphate proteoglycan, versican, in rat TMJ discs during postnatal development (2-32 weeks) were examined using Western blot analysis, real-time quantitative PCR and immunohistochemistry. Western blot analysis showed that rat TMJ discs predominantly expressed one isoform (V1) and the core protein sharply increased after birth, reached a peak at 8 weeks, and then gradually decreased up to 32 weeks. Real-time quantitative PCR with TaqMan probes indicated that mRNA expression of versican was highest at 2 weeks and gradually decreased with growth. An immunohistochemical study showed that staining for versican was weak and evenly distributed in TMJ discs at 2 weeks. Regional differences in staining for versican became prominent after 8 weeks; staining was intense in the anterior and posterior peripheral attachments, and weak in the central part of the discs. These results demonstrate that growth-related changes and regional differences exist in the expression of versican in the TMJ discs of growing rats, and these probably reflect the changes in the biomechanical environment caused by the development of orofacial functions.

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An immunohistochemical study of the localization of biglycan, decorin and large chondroitin-sulphate proteoglycan in adult rat temporomandibular joint disc

Cited by 37 publications

References 35 publications

Quantitative analysis and comparative regional investigation of the extracellular matrix of the porcine temporomandibular joint disc

Quantitative analysis and comparative regional investigation of the extracellular matrix of the porcine temporomandibular joint disc

Degenerative changes of articular cartilage in association with mechanical stimuli

Expression and localization of versican during postnatal development of rat temporomandibular joint disc

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