An immunohistochemical study of the insulin-, glucagon- and somatostatin-immunoreactive cells in the developing pancreas of the chicken embryo

Ku, Sae Kwang; Lee, J.-H.; Lee, H.-S.

doi:10.1054/tice.1999.0086

Cited by 20 publications

(14 citation statements)

References 19 publications

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“…The Endocrine region of the pancreas in avian species generally consists of large-diameter alpha islets, a higher number of beta islets [6,17] , and a few or no mixed islets [5,14] . In many studies, the number of endocrine islets in both avian and mammalian species is higher in the splenic lobe (tail region) than the other lobes of the pancreas [14,18] .…”

Section: Discussionmentioning

confidence: 99%

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Kara¹,

Tekiner²,

Şimşek³

et al. 2014

Kafkas Univ Vet Fak Derg

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The distribution and location of endocrine cells were determined in the dorsal, ventral, and splenic lobes of sparrowhawk pancreas using immunohistochemical methods. In this study, pancreatic tissues were stained with anti-insulin, anti-glucagon, anti-somatostatin, anti-gastrin, and anti-serotonin primary antibodies using the streptavidin-biotin-peroxidase method. The results showed that the numbers of glucagon-(alpha cell), insulin-(beta cell), and somatostatin-(delta cell) releasing cells were high and located in the splenic lobe of the pancreas. These endocrine cells were grouped into alpha, beta, and mixed islets. Alpha islets were mainly composed of alpha and delta cells and also occasionally beta cells. Beta islets contained numerous beta cells and a few delta and alpha cells. Furthermore, in the exocrine tissue were showed as only one cell or 2-3 gastrin immunopositive cell groups, whereas serotonin immunopositive cells were not found anywhere in the exocrine and endocrine pancreas. In conclusion, the endocrine islet types, endocrine cell localizations and lobe numbers of pancreas in sparrowhawks are similar to predator bird species, but are determined different to domestic fowls. Keywords: Insulin, Glucagon, Somatostatin, Gastrin, Pancreas, Sparrowhawk Atmaca (Accipiter nisus) Pankreas Dokusu Endokrin Hücrelerin Dağılımı ve Lokalizasyonu ÖzetBu çalışmada, atmaca pankreasının dorsal, ventral ve splenik loblarında bulunan endokrin hücrelerin dağılımı immunhistokimysal metotlarla belirlenmiştir. Pankreas dokusu anti-glukagon, anti-insulin, anti-gastrin ve anti-somatostatin primer antikorları kullanılarak streptavidin-biotin peroxidase metodu ile boyandı. Çalışmada, glukagon (alfa hücresi), insülin (beta hücresi) ve somatostatin (delta hücresi) salgılayan hücrelerin splenik lobda sayısının en fazla olduğu saptandı. Atmalarda pankreasın endokrin bölümlerinin alfa, beta ve miks adacıklar halinde gruplaştıkları saptandı. Beta adacıklarının çok sayıda beta hücresi, az sayıda delta ve alfa hücrelerini içerdiği belirlendi. Alfa adacıkları, genellikle alfa ve delta hücrelerinden nadiren de beta hücrelerinden oluşuyordu. Ayrıca, gastrin pozitif hücreler, sadece ekzokrin pankreasta 1 ya da 2-3 adet hücre grubu halinde bulunurken, seratonin pozitif hücrelere ise ekzokrin ve endokrin pankreasın herhangi bir bölümünde rastlanmadı. Sonuç olarak, atmaca pankreasının lop sayısı, endokrin adacık tipleri ve endokrin hücre lokalizasyonu açısından etçil kuşlarla benzerliği, evcil kuş türleriyle de farklılıkları belirlenmiştir.

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Section: Discussionmentioning

confidence: 99%

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Kara¹,

Tekiner²,

Şimşek³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…Moreover, quail pancreas is containing of third lobe positioned between dorsal and ventral lobes, but neighbor with the descending loop of the duodenum (24). The pancreas is composed of exocrine and endocrine compartment; the exocrine tissue, which secretes pancreatic digestive juice, and the endocrine tissue, which secretes the hormones insulin, glucagons, somatostatin and pancreatic polypeptide for the control of the carbohydrate metabolism (12,16).…”

Section: Introductionmentioning

confidence: 99%

“…The insulin and glucagon hormones produce in the greatest amounts by beta and alpha cells which decrease and increase blood glucose levels, respectively. D cells release somatostatin which inhibit the release of glucagon and insulin hormones, but pancreatic polypeptide hormone produced by PP cells inhibits the exocrine pancreas secretions (10,12,14,15,19). The endocrine pancreas of the fowl is make up of small and large islets called alpha, beta and mixed islets (5,14).…”

Section: Introductionmentioning

confidence: 99%

Localization of insulin immunpositive cells and histochemical structure of the pancreas in falcons (Falco Anaumanni)

Şimşek¹,

Bayraktaroğlu²,

Altunay³

2009

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The present study was conducted to determine the regional distribution of insulin immunpositive cells and histochemical structure of pancreas in falcon (Falco Anaumanni). Falcon pancreas was composed of three different parts as dorsal, ventral and splenic lobes. Those lobes contained acinar cells, pancreatic ducts, alpha, beta and mixed islets. Alpha islets were determined 3-4 unit as large islets in centre of the splenic lobe, but a few as small islets in dorsal and ventral lobes. Beta islets were widespread as small clusters in all lobes which were especially 7-8 units in periphery of the splenic lobe. Furthermore, mixed islets were found abundantly as small or large islets in all lobes. In the streptavidin biotin peroxidase staining of the pancreatic lobes, insulin immunpositive cells were numerous in the centre of beta islets, on the other hand these cells were detected as only one cell or 3-8 immunpositive cell groups in both alpha islets and exocrine tissue. In conclusion, the morphology and insulin immunpositive cells of pancreas in falcons are similar to duck, whereas are different from other avian species such as chicken, goose, and quail.Key words: Falcon, immunohistochemistry, insulin immunpositive cells, pancreas. Şahinlerde (Falco Anaumanni) pankreasın histokimyasal yapısı ve insülin immunpozitif hücrelerin lokalizasyonuÖzet: Bu çalışma, şahin (Falco Anaumanni) pankreasının histokimyasal yapısı ve insülin immunpozitif hücrelerin bölgesel dağılımını belirlemek için yapıldı. Şahin pankreası dorsal, ventral ve dalak lobu olarak 3 farklı lobdan oluşuyordu. Bu loplar asiner hücreler, akıtıcı kanallar, alfa, beta ve mix adacıkları içermekteydi. Alfa adacıkları dalak lobunun merkezinde 3-4 adet büyük adacık halinde gözlenirken, dorsal ve ventral lopta az sayıda küçük adacıklar halinde belirlendi. Beta adacıkları özellikle dalak lobunun periferinde 7-8 adet küçük adacıklar halinde bütün loplarda çok sayıda tespit edildi. Ayrıca, miks adacıklarının küçük ya da büyük adacıklar halinde pankreasın bütün loplarında çok yaygın bir şekilde bulunduğu saptandı. Pankreatik lobların streptavidin-biotin peroksidaz boyamalarında, insulin immunpozitif hücreler beta adacıkları merkezinde çok sayıdaydı diğer yandan bu hücreler hem alfa adacıklarında hem de ekzokrin dokuda yalnız tek bir hücre ya da 3-8 hücrelik gruplar halinde görüldü. Sonuç olarak şahin pankreasının morfolojik ve insulin pozitif hücreler açısından ördek pankreası ile benzer olduğu, tavuk, kaz ve bıldırcın gibi diğer kanatlı türlerinin pankreaslarından farklı olduğu tespit edilmiştir. Anahtar sözcükler: İmmunhistokimya, insulin pozitif hücreler, pancreas, şahin.

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“…We used an ELISA-based assay kit to detect blood insulin levels (Figure 3(a)). Even though the embryonic pancreas is not fully developed, insulin-positive pancreatic cells are present in chicken embryos from E10 [26]. At E18, blood insulin level was low in the control group but was significantly decreased in STZ-injected embryos (Figure 3(a)).…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, cultured human islets are also highly resistant to STZ [24, 25]. In order to successfully induce diabetes in chickens, we took advantage of the fact that the pancreas is not fully developed in chicken embryos [26], but cone photoreceptors are functional during the late embryonic stages [27–30]. We injected STZ into the amnion layer in ovo and successfully induced type 1 diabetes.…”

Section: Introductionmentioning

confidence: 99%

Chicken Embryos as a Potential New Model for Early Onset Type I Diabetes

Shi

Huang

et al. 2014

Journal of Diabetes Research

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Diabetic retinopathy (DR) is the leading cause of blindness among the American working population. The purpose of this study is to establish a new diabetic animal model using a cone-dominant avian species to address the distorted color vision and altered cone pathway responses in prediabetic and early diabetic patients. Chicken embryos were injected with either streptozotocin (STZ), high concentration of glucose (high-glucose), or vehicle at embryonic day 11. Cataracts occurred in varying degrees in both STZ- and high glucose-induced diabetic chick embryos at E18. Streptozotocin-diabetic chicken embryos had decreased levels of blood insulin, glucose transporter 4 (Glut4), and phosphorylated protein kinase B (pAKT). In STZ-injected E20 embryos, the ERG amplitudes of both a- and b-waves were significantly decreased, the implicit time of the a-wave was delayed, while that of the b-wave was significantly increased. Photoreceptors cultured from STZ-injected E18 embryos had a significant decrease in L-type voltage-gated calcium channel (L-VGCC) currents, which was reflected in the decreased level of L-VGCCα1D subunit in the STZ-diabetic retinas. Through these independent lines of evidence, STZ-injection was able to induce pathological conditions in the chicken embryonic retina, and it is promising to use chickens as a potential new animal model for type I diabetes.

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An immunohistochemical study of the insulin-, glucagon- and somatostatin-immunoreactive cells in the developing pancreas of the chicken embryo

Cited by 20 publications

References 19 publications

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Localization of insulin immunpositive cells and histochemical structure of the pancreas in falcons (Falco Anaumanni)

Chicken Embryos as a Potential New Model for Early Onset Type I Diabetes

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