Distribution and Location of Endocrine Cells in the Pancreas of the Sparrowhawk, Accipiter nisus

Abstract

The distribution and location of endocrine cells were determined in the dorsal, ventral, and splenic lobes of sparrowhawk pancreas using immunohistochemical methods. In this study, pancreatic tissues were stained with anti-insulin, anti-glucagon, anti-somatostatin, anti-gastrin, and anti-serotonin primary antibodies using the streptavidin-biotin-peroxidase method. The results showed that the numbers of glucagon-(alpha cell), insulin-(beta cell), and somatostatin-(delta cell) releasing cells were high and located in the splenic lobe of the pancreas. These endocrine cells were grouped into alpha, beta, and mixed islets. Alpha islets were mainly composed of alpha and delta cells and also occasionally beta cells. Beta islets contained numerous beta cells and a few delta and alpha cells. Furthermore, in the exocrine tissue were showed as only one cell or 2-3 gastrin immunopositive cell groups, whereas serotonin immunopositive cells were not found anywhere in the exocrine and endocrine pancreas. In conclusion, the endocrine islet types, endocrine cell localizations and lobe numbers of pancreas in sparrowhawks are similar to predator bird species, but are determined different to domestic fowls. Keywords: Insulin, Glucagon, Somatostatin, Gastrin, Pancreas, Sparrowhawk Atmaca (Accipiter nisus) Pankreas Dokusu Endokrin Hücrelerin Dağılımı ve Lokalizasyonu ÖzetBu çalışmada, atmaca pankreasının dorsal, ventral ve splenik loblarında bulunan endokrin hücrelerin dağılımı immunhistokimysal metotlarla belirlenmiştir. Pankreas dokusu anti-glukagon, anti-insulin, anti-gastrin ve anti-somatostatin primer antikorları kullanılarak streptavidin-biotin peroxidase metodu ile boyandı. Çalışmada, glukagon (alfa hücresi), insülin (beta hücresi) ve somatostatin (delta hücresi) salgılayan hücrelerin splenik lobda sayısının en fazla olduğu saptandı. Atmalarda pankreasın endokrin bölümlerinin alfa, beta ve miks adacıklar halinde gruplaştıkları saptandı. Beta adacıklarının çok sayıda beta hücresi, az sayıda delta ve alfa hücrelerini içerdiği belirlendi. Alfa adacıkları, genellikle alfa ve delta hücrelerinden nadiren de beta hücrelerinden oluşuyordu. Ayrıca, gastrin pozitif hücreler, sadece ekzokrin pankreasta 1 ya da 2-3 adet hücre grubu halinde bulunurken, seratonin pozitif hücrelere ise ekzokrin ve endokrin pankreasın herhangi bir bölümünde rastlanmadı. Sonuç olarak, atmaca pankreasının lop sayısı, endokrin adacık tipleri ve endokrin hücre lokalizasyonu açısından etçil kuşlarla benzerliği, evcil kuş türleriyle de farklılıkları belirlenmiştir.

Histology and immunofluorescent study of the pancreas in lovebird (Agapornis personatus)

“…The present finding indicates an even distribution of delta cells throughout the islets. This observation comes in line with those reported in sparrow hawks (Kara et al., 2014) and gooses (Gulmez et al., 2004). Furthermore, the higher concentration of delta cells as compared to beta cells was an interesting finding.…”

Histological and immunohistochemical study of the endocrine pancreas of duck (Anas platyrhnchos)

“…The observation of the current study detected that the islets in the duck pancreas were more concentrated in the spleniclobe than in the other lobes, which are found as a big group of all types of islets, as noted in (Figure 6). The finding of the present study agrees with Kara et al (2014) in the Sparrowhawk, but this result disagreement with Rawdon (1998) in the chicken, Gülmez et al (2004) in the goose, those researchers stated that the endocrine tissue is more concentrated in the third and splenic lobes. Moreover, This result disagrees with what Saadatfar et al (2011) reported in their research that pancreatic islets were concentrated in the central portion of the ventral lobe.…”

A Histochemical and Immunohistochemical Study on the Gastrointestinal Tract of Sparrowhawk (Accipiter nisus)

“…Also, in total, 20 different areas were examined in each section of the birds. Then the distribution of these densities throughout the anatomical regions was scored according to the approach described in a previous study (Kara et al, 2014). The densities were scored as (-) absent, (+) low-intensity, (++) medium-intensity and (+++) high intensity.…”