An Immunohistochemical Study of Sensory and Autonomic Innervation of the Dog Tongue with Special Reference to Substance P- and Calcitonin Gene-Related Peptide-Containing Fibers in Blood Vessels and the Intralingual Ganglia.

Hino, Naoki; Masuko, Sadahiko; Katsuki, Tsutomu

doi:10.1679/aohc.56.505

Cited by 18 publications

(8 citation statements)

References 35 publications

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“…In the latter report it is not possible to determine the frequency of this observation or the precise location of the cells. In general, across various species, it is suggested that the intralingual ganglion cells function as a parasympathetic supply to posterior tongue glands and the lingual vasculature (Fitzgerald and Alexander, 1969;Graziadei and Graziadei, 1978;Kuder, 1984;Hino, et al, 1993).…”

Section: Autonomic Ganglion Cells In the Posterior Rat Tonguementioning

confidence: 99%

Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia

Mbiene¹,

MacCallum²,

Mistretta³

1997

J. Comp. Neurol.

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Taste buds on the mammalian tongue are confined to the epithelium of three types of gustatory papillae: the fungiform, circumvallate, and foliate. The gustatory papillae are composed of an epithelium that covers a broad connective tissue core, with extensive innervation to taste bud and nongustatory epithelial locations. Although the temporal sequence of gustatory papilla development is known for several species, factors that regulate initiation, growth, and maintenance of the papillae are not understood. We tested the hypothesis that sensory innervation is required for the initial formation and early morphogenesis of fungiform papillae in a patterned array. An organ culture of the embryonic rat tongue was developed to provide an in vitro system for studying mechanisms involved in fungiform papilla morphogenesis in patterns on the anterior tongue. Tongues were dissected from embryos at 13 days of gestation (E13), a time when the tongue has not yet fully formed and gustatory papillae have not yet appeared, and at 14 days of gestation (E14), when the tongue is well formed and papillae make their initial morphological appearance. Dissected tongues were maintained at the gas/liquid interface in standard organ culture dishes, fed with DMEM/F12 plus 2% B-27 supplement and 1% fetal bovine serum. After 1, 2, 3, or 6 days in culture, tongues were processed for scanning electron or light microscopy, or immunocytochemistry. Tongues cultured from E13 or E14 underwent extensive morphogenesis and growth in vitro. Furthermore, fungiform papillae developed on these tongues on a culture day equivalent to E15 in vivo; that is, after 2 days for cultures begun at E13 and 1 day for those begun at E14. Because E15 is the characteristic time for gustatory papilla formation in the intact embryo, results demonstrate that the cultured tongues retain important temporal information related to papilla development. In addition, fungiform papillae formed in the tongue cultures in the stereotypic pattern of rows. The papillae were large structures with epithelial and mesenchymal cell integrity, and an intact epithelial basement membrane was indicated with laminin immunoreactivity. The cultures demonstrate that gustatory papilla morphogenesis can progress in the absence of an intact sensory innervation. To exclude a potential developmental role for autonomic ganglion cells that are located in the posterior rat tongue, cultures consisting of only the anterior half of E14 tongues were established. Fungiform papilla development progressed in half tongues in a manner directly comparable to whole tongue cultures. Therefore, robust, reproducible development of fungiform papillae in patterns is supported in rat tongue cultures from E13 or E14, without inclusion of intact sensory or major, posterior tongue autonomic ganglia. This is direct evidence that papillae will form and develop further in vitro without sensory ganglion support. The data also provide the first detailed account of in vitro development of the entire embryonic tongue.

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Section: Autonomic Ganglion Cells In the Posterior Rat Tonguementioning

confidence: 99%

Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia

Mbiene¹,

MacCallum²,

Mistretta³

1997

J. Comp. Neurol.

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“…Sbarbati et al (2002) could not detect SP‐IR nerve cell bodies either in the superficial or in the deep portions of the intrinsic nervous system of rat tongue. In contrast, in the findings of Hino et al (1993) and Oomori et al (1995), SP‐IR neurons were demonstrated in the dog and in the rat at the base of the vallate papillae, near the von Ebner's glands and among intralingual muscles.…”

mentioning

confidence: 73%

“…The presence of nerve ganglia in the mammalian tongue has been known for many years, and their relationship to arteries and to salivary glands as well as to taste buds has been described previously (Gairns and Garben, 1952; Fitzgerald and Alexander, 1969). In intralingual ganglionic cells, an intense reaction for acetylcholinesterase and nicotine amide adenine dinucleotide phosphate diaphorase was revealed (Yoshida and Toda, 1997), and they were also IR for VIP, NPY, and SP (Hino et al, 1993; Oomori et al, 1995). Our results showed that intralingual nerve cell bodies were also IR for SP and that they were located mainly in the connective tissue beneath the epithelium of the vallate papilla.…”

Section: Discussionmentioning

confidence: 99%

“…In several animal models, a large number of different neuropeptide containing nerve fibers, e.g., substance P (SP), calcitonin gene‐related peptide, vasoactive intestinal polypeptide (VIP), and peptide histidine isoleucine amide, were demonstrated in the adult lingual epithelium, especially in and around the taste buds (Lundberg et al, 1979; Nagy et al, 1982; Duner‐Engstrom et al, 1986; Luts et al, 1990; Astbäck et al, 1997; Matsuda et al, 1997). All these reports suggested that these neuropeptides, especially SP, might be involved in the maintenance of taste buds or even in gustatory events as well as in the deep sensation of gustatory papillae (Lundberg et al, 1979; Nishimoto et al, 1985; Hino et al, 1993; Kusakabe et al, 1998). Somatosensory sensations (e.g., stinging, burning, cooling, and sharpness) can be induced by many types of food.…”

mentioning

confidence: 99%

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Morphological evidence of sensory neurons in the root of the rat tongue

2005

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In our previous studies, a large number of substance P (SP)-immunoreactive (IR) nerve fibers were detected in the rat tongue and their number increased after inflammation, suggesting that these fibers might be involved in the axon reflex. Therefore, in this study, we have examined the different neuropeptide-containing nerve elements by light, electron, and confocal laser microscopy. SP, vasoactive intestinal polypeptide (VIP), and neuropeptide Y (NPY) IR varicose fibers were numerous compared with other ones. Small groups of ganglia with perikarya IR for SP, VIP, NPY, galanin, and somatostatin were observed. The SP-IR nerve cell bodies were mainly located in the tunica propria just below the epithelial lining. Doublelabeling immunohistochemistry showed that the intrinsic SP-IR neurons did not colocalize VIP. The SP containing nerve terminals were observed in and below the epithelium as well as in very close contact to or making real synapses with other neurons in the intralingual ganglion. Our data confirmed the possibility of intrinsic sensory neurons, which might be the afferent branch of the intralingual reflex arch, while the VIP-and NPY-IR neurons located in the salivary glands, around the blood vessels, and in the muscle layer might constitute the efferent site of this reflex.

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“…Low-threshold mechanoreceptors have large cell bodies in the TG and corpuscular endings in the vibrissa, facial skin, and oral mucosa [Renehan and Munger, 1986;Rice et al, 1986;Ichikawa et al, 1994Ichikawa et al, , 1997Sugimoto, 1997, 2003;Iino et al, 1998]. Oral and craniofacial structures are also innervated by the sympathetic and parasympathetic ganglia [Hino et al, 1993;Takai et al, 1999;Ichikawa and Sugimoto, 2001b]. Sympathetic postganglionic neurons are located in the superior cervical ganglion (SCG).…”

Section: Introductionmentioning

confidence: 99%

TRPC1, TRPC3, and TRPC4 in Rat Orofacial Structures

Fujita

Sato²,

Yajima

et al. 2017

Cells Tissues Organs

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TRPC (transient receptor potential cation channel subfamily C) members are nonselective monovalent cation channels and control Ca²⁺ inflow. In this study, immunohistochemistry for TRPC1, TRPC3, and TRPC4 was performed on rat oral and craniofacial structures to elucidate their distribution and function in the peripheries. In the trigeminal ganglion (TG), 56.1, 84.1, and 68.3% of sensory neurons were immunoreactive (IR) for TRPC1, TRPC3, and TRPC4, respectively. A double immunofluorescence method revealed that small to medium-sized TG neurons co-expressed TRPCs and calcitonin gene-related peptide. In the superior cervical ganglion, all sympathetic neurons showed TRPC1 and TRPC3 immunoreactivity. Parasympathetic neurons in the submandibular ganglion, tongue, and parotid gland were TRPC1, TRPC3, and TRPC4 IR. Gustatory and olfactory cells were also IR for TRPC1, TRPC3, and/or TRPC4. In the musculature, motor endplates expressed TRPC1 and TRPC4 immunoreactivity. It is likely that TRPCs are associated with sensory, autonomic, and motor functions in oral and craniofacial structures.

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An Immunohistochemical Study of Sensory and Autonomic Innervation of the Dog Tongue with Special Reference to Substance P- and Calcitonin Gene-Related Peptide-Containing Fibers in Blood Vessels and the Intralingual Ganglia.

Cited by 18 publications

References 35 publications

Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia

Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia

Morphological evidence of sensory neurons in the root of the rat tongue

TRPC1, TRPC3, and TRPC4 in Rat Orofacial Structures

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