Morphological evidence of sensory neurons in the root of the rat tongue

Fehér, Erzsébet; Batbayar, Bayarchimeg; Zelles, T

doi:10.1002/ar.a.20215

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…The immunoreactive cells have been shown to be present in the jugular ganglia of anurans (Fukuda and Inoue, )). Although immunoreactive cells have been observed in the rat tongue (Fehér et al, ), immunohistochemical evidence of the SP‐immunoreactive cells in the anuran tongue is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ₂‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Tadokoro

Ando

Kawahara

et al. 2016

The Anatomical Record

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Previous studies have found a few intralingual ganglionic cells that were immunoreactive to vasoactive intestinal polypeptide (VIP) in the frog. A recent study reported a large number of such cells, and the possibility of the release of substance P (SP) from these. The aim of the present study was to investigate the distribution, origin, and colocalization of VIP-and SP-immunoreactive nerves in the tongue of the bullfrog, R. catesbeiana. In addition, the study also examined the colocalization of SP and phospholipase Cb 2 (PLCb 2 ) in the tongue and jugular ganglion. VIP immunoreactivity was seen in unipolar cells that were sparse in nerve bundles in the submucosal and muscle layers. The density of VIP-immunoreactive cells was approximately 4.8 cells/mm 3 . Their fibers terminated in the vicinity of the epithelial basal layer of the fungiform papillae. SP immunoreactivity was not seen in the VIPimmunoreactive cells, but was observed in pseudounipolar cells in the jugular ganglion. The SP fibers terminated close to the free surface, showing spindle-and button-like profiles. Transection of glossopharyngeal nerve resulted in the persistence of VIP-immunoreactive cells and the disappearance of SP-immunoreactive fibers in the tongue. SP immunoreactivity was co-expressed with PLCb 2 in both the tongue and jugular ganglia. No PLCb 2 immunoreactivity was seen in cells comprising the epithelial taste disk. These findings indicate that the origin of VIP nerve fibers are unipolar cells in the tongue, and SP and PLCb 2 fibers Abbreviations used: AChE 5 acetylcholinesterase; DAPI 5 6-diamidino-2-phenylindole, dihydrochloride; NOS 5 nitric oxide synthase; PB 5 phosphate buffer; PBS 5 phosphate buffered saline; SP 5 substance P; VIP 5 vasoactive intestinal polypeptide.Ethical approval: Our research conformed to the guidelines for the care and use of experimental animals established by the Ethical Committee of Animal Experiments of Matsumoto Dental University.Grant sponsor: National Institute for Physiological Sciences.

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Section: Introductionmentioning

confidence: 99%

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ₂‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Tadokoro

Ando

Kawahara

et al. 2016

The Anatomical Record

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Morphological evidence of local reflex arc in the rat’s tongue

Altdorfer

Zelles

Pongor

et al. 2012

Acta Physiologica Hungarica

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Lingual components of the autonomic nervous system are considered to be the most rostral portion of the enteric nervous system. Therefore our aim was to study the intrinsic nerve cell bodies and synapses using immunohisto-, immunocytochemical methods. Several small groups of ganglia with cell bodies immunoreactive (IR) for vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and substance P (SP) were observed just below the gustatory epithelium. A few somatostatin and galanin IR nerve cell bodies were also found. Many IR cell bodies were also demonstrated in the glands and next to blood vessels. Some of these cell bodies were multipolar and some of them were small neurons with an ovoid shape having only one process. Cell bodies positive for calcitonin gene-related peptide (CGRP) were detected neither in the superficial nor in the deep portion. Electronmicroscopical analysis demonstrated different IR nerve fibres having axo-somatic and axo-dendritic synapses with other immunonegative cells. In a few cases VIP IR nerve processes were found to synaptize with other VIP positive nerve cell bodies. These results support the existance of intralingual reflex in the tongue, where the ganglia might have an integrative role of the different neuropeptide containing nerve fibres.

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Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia

et al. 2023

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Huntingtin-associated protein 1 (HAP1) is a determinant marker for the stigmoid body (STB), a neurocytoplasmic physiological inclusion. STB/HAP1 enriched areas in the brain/spinal cord are usually protected from neurodegenerative diseases, whereas the regions with tiny amounts or no STB/HAP1 are affected. In addition to the brain/spinal cord, HAP1 is highly expressed in the myenteric/submucosal plexuses of the enteric nervous system in the gastrointestinal tract. The tongue is attached to the pharynx by the hyoid bone as an extension of the gastrointestinal system. To date, the immunohistochemical distribution and neurochemical characterization of HAP1 have not been elucidated in the lingual ganglia. Using immunohistochemistry and light microscopy, our current study demonstrates the expression and immunohistochemical phenotype of HAP1 in the lingual ganglia of adult mice. We showed that HAP1 was profoundly distributed in the intralingual ganglion (ILG) and the ganglia near the root of the tongue (which we coined as “lingual root ganglion”; LRG). Neurons in ILG and LRG exhibited high coexpression of HAP1 with NOS or ChAT. Furthermore, most HAP1-immunoreactive neurons contained SP, CGRP, and VIP immunoreactivity in both ILG and LRG. The current results might serve as an essential base for future studies to elucidate the pathological/physiological functions of HAP1 in the lingual ganglia.

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Morphological evidence of sensory neurons in the root of the rat tongue

Cited by 3 publications

References 29 publications

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ₂‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ₂‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Morphological evidence of local reflex arc in the rat’s tongue

Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia

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Morphological evidence of sensory neurons in the root of the rat tongue

Cited by 3 publications

References 29 publications

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ2‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ2‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Morphological evidence of local reflex arc in the rat’s tongue

Immunohistochemical Distribution and Neurochemical Characterization of Huntingtin-Associated Protein 1 Immunoreactive Neurons in the Adult Mouse Lingual Ganglia

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Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ₂‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana

Distribution and Origin of VIP‐, SP‐, and Phospholipase Cβ₂‐Immunoreactive Nerves in the Tongue of the Bullfrog, Rana catesbeiana