An immunohistochemical study examining the role of collagen type VI in the rodent periodontal ligament

Sloan, Philip; Carter, D. H.; Cm, Kielty; Shuttleworth, C. Adrian

doi:10.1007/bf00159289

Cited by 17 publications

(9 citation statements)

References 28 publications

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“…Further related studies presented elsewhere on the periodontal ligament (Sloan et al, 1993) and its associated tissues (Sloan & Carter, in prep), confirm our view that the technique described above may have a wider use than was originally envisaged and that its application may be extended to the histological and histopathological analysis of the dental tissues. …”

Section: Discussionsupporting

confidence: 75%

The cryomicrotomy of rat dental tissues: a technique for histological and immunohistochemical analysis

1994

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We have applied a method developed for the cryomicrotomy of non-decalcified bone to the histological preparation of the tooth and related dental tissues. Cryosections of rat mandible have been cut on a heavy-duty freezing microtome. Both cellular and extracellular structure was well preserved and the sections of tooth and bone appeared to be suitable for optical and scanning electron microscopy and for immunohistochemical analysis. However, there was an overall strong non-specific binding of immunohistochemical reagents to enamel which was not evident in the other mineralized tissues of the mandible. This may relate to important differences in the nature of this tissue.

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Section: Discussionsupporting

confidence: 75%

The cryomicrotomy of rat dental tissues: a technique for histological and immunohistochemical analysis

1994

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“…In addition, type VI collagen has been linked to the early events of chondrocyte differentiation (76), to the regulation of mesenchymal cell proliferation in vitro (77), and to ECM stabilization during development (36). It has also been hypothesized that collagen VI is important for chondrocyte proliferation and hypertrophy in cartilage (36, 78, 79). These studies in conjunction with our observation of the ubiquitous presence of type VI collagen in the growth plate (Fig.…”

Section: Discussionmentioning

confidence: 99%

Developmental and osteoarthritic changes in Col6a1‐knockout mice: Biomechanics of type VI collagen in the cartilage pericellular matrix

Alexopoulos

Youn

Bonaldo

et al. 2009

Arthritis & Rheumatism

169

170

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Objective. Chondrocytes, the sole cell type in articular cartilage, maintain the extracellular matrix (ECM) through a homeostatic balance of anabolic and catabolic activities that are influenced by genetic factors, soluble mediators, and biophysical factors such as mechanical stress. Chondrocytes are encapsulated by a narrow tissue region termed the "pericellular matrix" (PCM), which in normal cartilage is defined by the exclusive presence of type VI collagen. Because the PCM completely surrounds each cell, it has been hypothesized that it serves as a filter or transducer for biochemical and/or biomechanical signals from the cartilage ECM. The present study was undertaken to investigate whether lack of type VI collagen may affect the development and biomechanical function of the PCM and alter the mechanical environment of chondrocytes during joint loading.Methods. Col6a1 ؊/؊ mice, which lack type VI collagen in their organs, were generated for use in these studies. At ages 1, 3, 6, and 11 months, bone mineral density (BMD) was measured, and osteoarthritic (OA) and developmental changes in the femoral head were evaluated histomorphometrically. Mechanical properties of articular cartilage from the hip joints of 1-monthold Col6a1 ؊/؊ , Col6a1 ؉/؊ , and Col6a1 ؉/؉ mice were assessed using an electromechanical test system, and mechanical properties of the PCM were measured using the micropipette aspiration technique. Results. In Col6a1؊/؊ and Col6a1 ؉/؊ mice the PCM was structurally intact, but exhibited significantly reduced mechanical properties as compared with wildtype controls. With age, Col6a1 ؊/؊ mice showed accelerated development of OA joint degeneration, as well as other musculoskeletal abnormalities such as delayed secondary ossification and reduced BMD.Conclusion. These findings suggest that type VI collagen has an important role in regulating the physiology of the synovial joint and provide indirect evidence that alterations in the mechanical environment of chondrocytes, due to either loss of PCM properties or Col6a1 ؊/؊ -derived joint laxity, can lead to progression of OA.Articular cartilage is the tissue that lines the surfaces of diarthrodial joints and serves as the resilient, low-friction, load-bearing material for joint motion. A sparse population of cells-chondrocytes-maintains the extracellular matrix (ECM) of this tissue through a balance of anabolic and catabolic activities. The micromechanical environment of chondrocytes, in conjunction with biochemical factors (e.g., growth factors, cytokines) and genetic factors, plays an important role in cartilage homeostasis and, as a consequence, the health of the joint (1-3). Chondrocytes in articular cartilage are enclosed by a narrow region of tissue termed the "pericellular matrix" (PCM), which, together with the enclosed chondrocyte, has been termed the "chondron" (4-7). The PCM is characterized primarily as being the exclusive location of type VI collagen in normal cartilage, but proteoglycans, fibronectin, and types II and IX collagen are also pre...

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“…The rapid remodeling of intermediate collagen might be due, in part, to a dominant phagocytosis of the collagen fibrils in the rodent incisor periodontal ligament (Ten Cate and Deporter, 1975;Everts and Beertsen, 1988). It has also been proposed that the lack of collagen type VI in the middle zone of the ligament, which may play a role in maintaining the integrity of the fiber bundles, could facilitate the remodeling of the collagen fibers during the eruptive process (Sloan et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Polarized light microscopic analyses of collagen fibers in the rat incisor periodontal ligament in relation to areas, regions, and ages

Komatsu¹,

Mosekilde²,

Viidik³

et al. 2002

Anat. Rec.

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We prepared decalcified sagittal sections (20 m thick) from the incisal, middle, and basal regions of the mandibular incisor of male Wistar rats aged 2, 6, 12, and 24 months, and examined the sections using polarized light microscopy. Most of the birefringent fibers appeared to run obliquely across the periodontal ligament. Birefringent fibers running parallel to the long axis of the incisor were also found in the intermediate area of the ligament. Similar fiber architecture was observed in all four age groups. Quantitative analysis showed that the retardation values of collagen were higher in the bone-and tooth-related areas and lower in the intermediate area of the ligament. The values for the bone-and tooth-related areas increased from the basal toward the incisal regions in all four age groups. Age-related changes in the retardation values were found only in the incisal region of the incisor. In the incisal region, the values for the bone-and tooth-related areas increased markedly from 2-24 months of age, whereas those for the intermediate area increased slightly but significantly with age. Our findings indicate that the degrees of molecular organization and alignment of collagen fibers in the bone-and tooth-related areas of the ligament are higher than those in the intermediate area and increase near the incisal region and with age. It is also suggested that the collagen fibers in the intermediate area remain immature along the long axis of the incisor throughout the life span of the animal. Anat Rec 268: 381-387, 2002.

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An immunohistochemical study examining the role of collagen type VI in the rodent periodontal ligament

Cited by 17 publications

References 28 publications

The cryomicrotomy of rat dental tissues: a technique for histological and immunohistochemical analysis

The cryomicrotomy of rat dental tissues: a technique for histological and immunohistochemical analysis

Developmental and osteoarthritic changes in Col6a1‐knockout mice: Biomechanics of type VI collagen in the cartilage pericellular matrix

Polarized light microscopic analyses of collagen fibers in the rat incisor periodontal ligament in relation to areas, regions, and ages

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