The cryomicrotomy of rat dental tissues: a technique for histological and immunohistochemical analysis

Carter, D. H.; Sloan, Philip; Aaron, Jean E.

doi:10.1007/bf00157958

Cited by 9 publications

(6 citation statements)

References 20 publications

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“…Both cellular and extracellular structure are well preserved and the sections of tooth and bone appeared to be suitable for optical and scanning electron microscopy and for immunohistochemical analysis, However, it has the disadvantage of there being an overall strong non-specific binding of immunohistochemical reagents to enamel [6].…”

Section: Discussionmentioning

confidence: 99%

“…Both cellular and extracellular structures are well preserved and the sections of tooth and bone appeared to be suitable for optical and scanning electron microscopy and for immunohistochemical analysis. However, there is an overall strong non-specific binding of immunohistochemical reagents to enamel [6,7] therefore making the use of stains and histological examination doi: 10.7243/2053-5775-3-3 definition difficult.…”

Section: Introductionmentioning

confidence: 99%

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Histological preparation of teeth and tooth growth

Williamson¹

2015

Oral Biol Dent

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histological preparation of teeth and tooth growth

Williamson¹

2015

Oral Biol Dent

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“…Second, immunohistochemical findings in ectopic mineralization site was not evaluated in relation to mineralization in this study because the ectopic mineralization was stained with normal rabbit IgG. Although undecalcified frozen sections may preserve the antigenicity, non‐specific reactions for mineralized tissue were reported (Carter, Sloan, & Aaron, ). If the complete negative control is achieved by histological procedures, such as pretreatment of the section by EDTA (Carter et al, ), direct comparison between parameters of the immunohistochemistry and mineralization in ectopic mineralization site become possible.…”

Section: Discussionmentioning

confidence: 99%

“…Although undecalcified frozen sections may preserve the antigenicity, non‐specific reactions for mineralized tissue were reported (Carter, Sloan, & Aaron, ). If the complete negative control is achieved by histological procedures, such as pretreatment of the section by EDTA (Carter et al, ), direct comparison between parameters of the immunohistochemistry and mineralization in ectopic mineralization site become possible. Finally, quantification of immunohistochemical findings has no guarantee of accurate amount of expressed proteins.…”

Section: Discussionmentioning

confidence: 99%

Histomorphometry of ectopic mineralization using undecalcified frozen bone sections

Fujihara

Chiba

Nakagawa

et al. 2018

Microscopy Res & Technique

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To investigate the correlation between mineral formation and enhanced expressions of some proteins using undecalcified frozen bone sections. Histological studies have revealed that some proteins, such as BMP2, BMPR1A, and Connexin 43, are expressed in and around sites of ectopic ossification. However, the relationship between the expressed proteins considered to be associated with the ossification and mineral formation in vivo is not clear. Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1)-mutant spinal hyperostotic TWY mice and ICR mice as controls were euthanized after calcein labeling, and undecalcified frozen sections were obtained from the middle thoracic spine. Intervertebral disc areas were examined histologically and by measuring calcein-labeled areas and areas showing immunoreactivity for BMP2, BMPR1A, and Connexin 43. Calcein-labeled areas, indicating mineralization in the ectopic mineralization sites, were significantly larger in the mutant mice than in controls. The expression of Connexin 43 was elevated in the annulus fibrosus. Increases in the calcein-labeled areas was not correlated with increases in the areas showing immunoreactivity for Connexin 43 in the annulus fibrosus. There was no statistical correlation between enhanced immunohistochemical expression and elevated calcein-labeled areas. By applying the morphometrical analysis method using undecalcified frozen sections to ENPP1-mutant mice, quantitative evaluation of the mineralization and proteins expressed in the surrounding area in the same animal became possible. K E Y W O R D S BMP2, BMPR1A, Connexin 43, ENPP1, histomorphometry, mineralization, ossification of the posterior longitudinal ligament, undecalcified frozen section

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“…Cryomicrotomy. Carter et al (1994) used a method developed for cryomicrotomy of non-decalcified bone on the histological preparation of teeth and related tooth tissues. Sections of mandible from Sprague-Dawley rats were frozen instantaneously in isopentane chilled to -170oC with liquid nitrogen.…”

Section: Cutting-grinding Technique (Cgt)mentioning

confidence: 99%

Histological Techniques for the Study of the Dentogingival Junction: A Scoping Review Using the Anatomical Quality Assurance Checklist (AQUA)

Correa-Aravena¹,

Vásquez²,

Otzen³

et al. 2023

Int. J. Morphol.

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The dentogingival junction (DGJ) is an adaptation of the oral mucosa composed of epithelial and connective tissues intimately related with the mineralised tissues of the tooth. The histological evidence available is mainly based on studies in animals, separate evaluations of hard and soft tissues, and studies using conventional histological techniques that eliminate the enamel from preparations. The aim of this study was to carry out a review of the existing evidence on histological techniques available for study of the tooth and periodontium in conjunction in humans. A scoping review was carried out of the available literature referring to study of the tooth and the periodontium in conjunction in humans, in the Web of Science (WoS), EMBASE, Scopus and SciELO databases, using the terms "Histological Techniques"[Mesh]) and "Epithelial Attachment" [Mesh]. One hundred and fifty-nine articles were found, of which 54 were selected for fulltext reading. Ten were finally included in the qualitative synthesis, and we applied the Anatomical Quality Assurance (AQUA) checklist for analysis the methodological quality of the selected articles. The results showed that the only articles with a low risk of bias in all five domains according to the AQUA criteria corresponded to Silva et al. (2011) and Agustín-Panadero et al. (2020). Finally, we conclude that the quality of the histological sections to observe tissues that simultaneously contain the tooth and the periodontium, is conditioned by the selected technique and by the care required in certain specific tasks during the histological processing of the samples.

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The cryomicrotomy of rat dental tissues: a technique for histological and immunohistochemical analysis

Cited by 9 publications

References 20 publications

Histological preparation of teeth and tooth growth

Histological preparation of teeth and tooth growth

Histomorphometry of ectopic mineralization using undecalcified frozen bone sections

Histological Techniques for the Study of the Dentogingival Junction: A Scoping Review Using the Anatomical Quality Assurance Checklist (AQUA)

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