An Immunoenzymatic Method for the Determination of Ochratoxin A in Biological Liquids (Colostrum and Cow’s Milk)

Abstract

Ochratoxins are mycotoxins that have been extensively studied lately due to the multiple toxic effects such as nephrotoxicity, hepatotoxicity, and carcinogenicity. These toxins contaminate plant and animal foods and after ingestion they reach into body fluids. The method of competitive direct enzyme immunoassay, in the solid phase, was validated through the determination of specific parameters (performance, linearity, recovery percentage, limit of detection, limit of quantification). The validated method was used to determine ochratoxin A in colostrum and cow’s milk. The method applied for the determination of ochratoxin A was linear for the concentration range of 0.0–0.5 ng/mL, the value for the regression coefficient (r) was 0.9838. Ochratoxin A was present in 91.67% of the colostrum and in 93.33% of cow’s milk samples. The linearity of the method, demonstrated for very low concentrations of analyte, the detection limit as well as the limit of quantification recommend the method for the determinations of micro-pollutants from foods, including biological fluids.

Determination of Ochratoxin-A Detoxification and Antibiotic Resistance Potential of Acinetobacter calcoaceticus

“…1 These toxins can have harmful effects on human and animal health, including carcinogenic, mutagenic, teratogenic, and immunosuppressive effects. [2][3][4] Mycotoxins can also cause acute and chronic illnesses, including liver damage, respiratory distress, and neurological disorders. [5][6][7] The most common types of mycotoxins include aflatoxins, ochratoxins, fumonisins, deoxynivalenol (DON), zearalenone (ZEN), and patulin.…”

Bio-control on the contamination of Ochratoxin A in food: Current research and future prospects

“…The chemical structure of OTA consists of a 7-carboxy- 5-chloro -8-hydroxy-3, 4-dihydro- 3- methylcoumarin, linked through the 7-carboxy- group to L-β- phenylalanine by an amide bond. As one of the most important mycotoxins concern for human health, OTA exhibits nephrotoxic, mutagenic, immunotoxic, teratogenic and carcinogenic properties ( Cuciureanu et al, 2021 ; Hashemi et al, 2021 ). For a long time, the contamination of OTA has been monitored in a variety of foods, such as meat, eggs, fruit and vegetables, dairy products, milk-based baby formulae and infant cereals.…”