An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues

Xie, Yong; Zhang, Lei; Le, Tao

doi:10.1080/09540105.2017.1293013

Cited by 24 publications

(10 citation statements)

References 28 publications

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“…The LOD and the limit of quantification (LOQ), based on the results of 20 different blank animal tissue samples (chicken, pork, fish, and shrimp) were 0.15 and 0.31 μg/kg, respectively. The recovery was in the range of 76.3-98.4%, and the coefficient of variation was below 15.0% (Xie et al, 2017).…”

Section: Colloidal Gold Immunochromatographic Assaymentioning

confidence: 88%

Progress in immunoassays for nitrofurans detection

Yang

Liu

et al. 2020

Food and Agricultural Immunology

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Nitrofurans comprise a class of synthetic broad-spectrum antibacterial agents, which had been used extensively as therapeutic drugs and feed additives in animal husbandry. Because of the potential carcinogenic and mutagenic effects of these drugs and their metabolites, most countries have banned their use in food animals. However, driven by economic interests, the illegal use of nitrofurans poses a significant threat to human health. Therefore, to safeguard health of the consumer, it is of paramount importance to develop efficient, sensitive, and reliable detection methods to monitor nitrofuran and their metabolite residues in animal-derived foods. In this paper, the progress in immunoassays for nitrofuran residues is reviewed, including enzyme-linked immunosorbent assay, fluoroimmunoassay, chemiluminescence immunoassay, immunochromatography, and immunosensor assay. Basic principles and applications of these methods were described and compared in this paper. Furthermore, the future perspective on the improvement and development of the immunoassays for the detection of nitrofurans has been discussed.

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Section: Colloidal Gold Immunochromatographic Assaymentioning

confidence: 88%

Progress in immunoassays for nitrofurans detection

Yang

Liu

et al. 2020

Food and Agricultural Immunology

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“…The optical cut off value was used for qualitative determination for the LFI strip, optical cut off can be described as the lowest concentration of substrate that the color of the test line decays [15,16]…”

Section: Determination Of the Lateral Flow Immunoassay Performancementioning

confidence: 99%

Lateral Flow Immunoassay Based Colloidal Gold Nanoparticles for the Detection of Organophosphorous Pesticide (Propanoic) in Cow’s Milk

Haleem¹,

Haleem²,

Abass³

2021

DJPS

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Simple, inexpensive, and high sensitive Lateral Flow Immunoassay (LFI) based colloidal gold nanoparticles antigen-coated as the label was designed for the fast determination of organophosphorus pesticide in cow’s milk samples. The developed LFI detection strip consisted of four structural zones start from the sample pad, conjugate pad, detection zone, and finally absorbent pad. Immune-sensitive zone was synthesized and functionalized with Propanoic Hapten–OVA, Goat monoclonal-anti-Propanoic antibodies were produced. Propionic -cationized ovalbumin-HPR conjugates were fabricated and identified. The antibody optimal conditions were set and the influence of pH on the analytical properties of the antibody-based gold nanoparticles was also investigated. The detection limit of the LFI for Propanoic standard into milk samples were 0.5 and 100 ng/mL, respectively. The test development time for the LFI was less than 5 min, suitable for fast on-site measuring of Propanoic.

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“…However, this family of drugs and their metabolites are known to be carcinogenic and mutagenic [ 2 , 3 ], and as a result, the nitrofurans have been listed as prohibited veterinary drugs in farmed animals for food production by many countries and regions, including the European Union (EU), USA, and China [ 4 ]. Meanwhile, many studies have demonstrated that the nitrofurans are quite unstable in vivo and are rapidly metabolized [ 5 , 6 , 7 ]. The metabolites formed from the above nitrofurans are 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD), and semicarbazide (SEM) ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Preparation of Monoclonal Antibody and Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay and Fluorescence-Linked Immunosorbent Assay for Detecting 3-Amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in Edible Animal Tissue

et al. 2021

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To monitor the illegal used of furaltadone, a highly sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and fluorescence-linked immunosorbent assay (FLISA) based on a monoclonal antibody (mAb) were developed for the detection of 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ), the major metabolite of furaltadone in animal tissues. The highly specific mAb, which was very sensitive to a nitrophenyl derivative of AMOZ (2-NP-AMOZ) with IC50 values of 0.11 and 0.09 ng/mL for ic-ELISA and FLISA, respectively, was selected for the development of immunoassays. For both the ic-ELISA and FLISA for AMOZ-spiked experiments, acceptable recovery rates of 81.1–105.3% and coefficients of variation of 4.7–9.8% were obtained. In addition, results from both ic-ELISA and FLISA methods for spiked samples’ data showed excellent correlation coefficients ranging from 0.9652 to 0.9927. Meanwhile, the proposed ic-ELISA and FLISA for thirty spiked samples were confirmed by standard LC-MS/MS with high correlation coefficients of 0.9911 and 0.9921, respectively. These results suggest that the developed ic-ELISA and FLISA are valid and cost-effective tools for high-throughput monitoring methods for AMOZ residues in animal tissues.

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An immunochromatography test strip for rapid, quantitative and sensitive detection of furazolidone metabolite, 3-amino-2-oxazolidinone, in animal tissues

Cited by 24 publications

References 28 publications

Progress in immunoassays for nitrofurans detection

Progress in immunoassays for nitrofurans detection

Lateral Flow Immunoassay Based Colloidal Gold Nanoparticles for the Detection of Organophosphorous Pesticide (Propanoic) in Cow’s Milk

Preparation of Monoclonal Antibody and Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay and Fluorescence-Linked Immunosorbent Assay for Detecting 3-Amino-5-methylmorpholino-2-oxazolidinone (AMOZ) in Edible Animal Tissue

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