An immunochromatographic assay for the rapid detection of oxadixyl in cucumber, tomato and wine samples

Rapid and sensitive detection of chlordimeform in cucumber and tomato samples using an immunochromatographic assay

“…The synthesis of antigens is carried out by coupling active groups on small molecules such as carboxyl (–COOH), amino (–NH 2 ), and hydroxyl (–OH) with carrier proteins. 38 Considering that there is no active group in CDM, we modified its structure to form a hapten with an active group. When designing haptens, caution should be exercised to retain the original structure of CDM and fully expose its characteristic sites, thereby producing antibodies with high sensitivity and affinity.…”

An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

“…The preparation of colloidal gold-mAb refers to the Liu’s methods [ 23 ]. Colloidal gold with a diameter of 20 nm was prepared using the sodium citrate reduction method.…”

Development of a colloidal gold strip assay for the detection of total homocysteine in serum samples

“…The isotype of mAb 3B3 was determined using a commercial mouse immunoglobulin isotyping ELISA kit and the sensitivity and specificity of the mAb were measured by ic-ELISA, as described previously. 26 Briefly, standard solutions of SAH at 0, 0.1, 0.3, 1, 3, 9, 27, and 80 nM were used to construct a standard curve and the 50% inhibition concentration (IC 50 ) was obtained, representing the sensitivity of the mAb towards SAH. The specificity of the mAb was determined by evaluating the cross-reactivity (CR) of several compounds structurally similar to SAH including SAM, adenosine, Hcy, l -cysteine, and cystathionine.…”