An Immunochemical Method for Detecting Carcinomatous Secretion from Human Gastric Juice

Häkkinen, I

doi:10.1080/00365521.1966.11800610

Cited by 25 publications

(12 citation statements)

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“…These subjects show in their FSA glycoproteins properties observed among gastric cancer patients, although the FSA glycoproteins of the latter group can often be seen to run and precipitate in a single pool. The physico-chemical and antigenic structure of the acid glycoproteins in the young healthy controls is quite different [7,15]. This group is character ized by absence of FSA, strong positivity for blood group antigens to gether with N" antigen (which is the normal organ-specific antigen of the upper alimentary canal acid glycoproteins) as well as monodispersity of macromolecules.…”

Section: Discussionmentioning

confidence: 99%

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Gastric Fetal Sulphoglycoprotein Antigen (FSA) and Blood Group Antigens A and B

Häkkinen¹

1974

Int Arch Allergy Immunol

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Acid gastric glycoproteins deriving from 180 non-cancerous FSA secretors, 74 gastric cancer patients (FSA positives), and 28 young controls (FSA negatives), all in blood group A or B were analyzed for content of blood group antigens and for the relation of FSA (or N_o in young controls) to these. The Ouchterlony analysis revealed six different combinations in the same glycoprotein molecules or separately. There seemed to be a trend towards disappearance of blood group antigens in gastric cancer, although some of the non-cancerous FSA positives and gastric cancer samples showed quite a similar polydisperse picture. The glycoproteins deriving from young controls were strictly monodisperse and showed a strong positivity for blood group antigens. The practical importance of the present data lies in the fact that they provide the possibility of following the FSA blood group picture of non-cancerous FSA positives. This may warrant further clinical examinations as the picture alters.

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Section: Discussionmentioning

confidence: 99%

“…The study was restrict ed to the age group from 40 to 70 years. Acid glycoproteins were isolated front gastric juice, using precipitation with cetylpyridinium chloride [7,10]. The samples thus obtained were analyzed for FSA in Ouchterlony plates against a specific rab bit anti-FSA serum.…”

Section: Methodsmentioning

confidence: 99%

Gastric Fetal Sulphoglycoprotein Antigen (FSA) and Blood Group Antigens A and B

Häkkinen¹

1974

Int Arch Allergy Immunol

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“…If other antigens, such as organ-specific ones are also present, the chemical nature of their carrier molecule could thus be suggested. None of the gastric glycoprotein fractions tested be now resulted in blood group antibodies in the rabbit [11,12] although they were blood group active, and of apparently the same size as those originating from ovarian cyst [7,19], On the other hand, some of the six organ-specific antigens demonstrated until now very often seem to be present in these fractions and immunize the rabbit specifically as well [13]. In the same immunization conditions, blood group substances originating from ovarian cyst fluid stimulated the rabbit to form specific blood group antibodies in high titres.…”

Section: Resultsmentioning

confidence: 99%

“…The treatment of gastric juice, the primary purification of glycoproteins, and their separation from each other have been described elsewhere [7,8,11,12,14], Briefly, H akkinen/V irtanen 273 to freshly collected gastric juice, of which salivary contamination was excluded, 4 vol. of alcohol was added.…”

Section: Methodsmentioning

confidence: 99%

The Appearance of Blood Group and Organ-Specific Antigens in Human Gastric Glycoproteins

Häkkinen¹,

Virtanen²

1970

Int Arch Allergy Immunol

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“…The isolation and chemical analysis of gastric sulphoglycoproteins has recently been performed (Hakkinen, Hartiala, and Terho, 1965; Hakkinen, 1967), and the preservation of these macromolecules as intact as possible in the isolation procedure for immunological identification and for analysis with physico-chemical methods has been the aim in successive studies (Hakkinen, 1966(Hakkinen, , 1967. A highly sensitive method for localizing human gastric sulphoglycoproteins in tissue sections is the indirect immunofluorescence technique (Hakkinen, Gronroos, and Kunnas, 1967).…”

mentioning

confidence: 99%