An Immunochemical Investigation of the Allergens from &lt;i&gt;Ascaris suum &lt;/i&gt;Perienteric Fluid

McWilliam, Andrew S.; Stewart, G.A.; Turner, Kati

doi:10.1159/000234177

Cited by 7 publications

(2 citation statements)

References 17 publications

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“…It should be mentioned, however, that some high-MW (360,000) allergens have also been identified in man, using sera from naturally infected individuals [34,35]. At the present, it is not known why these components fail to trig ger a more prominent IgE response in animal models.…”

Section: Discussionmentioning

confidence: 96%

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Isolation of <i>Ascaris suum</i> Components Which Suppress IgE Antibody Responses

Soares

Mota²,

Macedo³

1992

Int Arch Allergy Immunol

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An extract from adult Ascaris suum was fractionated on a Sephadex G-200 column and individual elution fractions assessed for their ability to induce or suppress an IgE antibody response in mice immunized simultaneously with a heterologous antigen (ovalbumin). The results showed that, whilst fractions eluted in the first peak suppressed the anti-ovalbumin IgE antibody production, those eluted in the third peak induced a significant anti-Ascaris IgE response. The mixture of the two kinds of fractions resulted in suppression of anti-Ascaris IgE antibodies. The apparent molecular weight (MW) of the first and third peaks was 530,000 and 29,000 daltons, respectively. After delipidation, the extract still retained most of its biological activities. SDS-PAGE of this material showed selective loss of high-MW components when compared to the whole extract. No bands were observed in SDS-PAGE of peak I in contrast to peak III, which displayed several bands. Immunoblots of all these samples showed at least 3 bands above MW 150,000, which reacted with anti-peak I anti-serum, in the unfractionated extracts and in peak I, but not in peak III. When the extract was fractionated by affinity chromatography using anti-peak I antibodies as ligands, the immunogenic components were present in the effluent, whereas the suppressive components were recovered in the eluate.

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Section: Discussionmentioning

confidence: 96%

“…At the present, it is not known why these components fail to trig ger a more prominent IgE response in animal models. This is probably not due to the employment of unnatural hosts, since it was shown that in N. brasiliensis-infected rats (natural host) the allergenic activity was also associ ated with low-MW allergens [35].…”

Section: Discussionmentioning

confidence: 99%

Isolation of <i>Ascaris suum</i> Components Which Suppress IgE Antibody Responses

Soares

Mota²,

Macedo³

1992

Int Arch Allergy Immunol

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Ascaris and Allergy

Caraballo¹

2013

Ascaris: The Neglected Parasite

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Evaluation by the skin prick test of Anisakis simplex antigen purified by affinity chromatography in patients clinically diagnosed with Anisakis sensitization

Rodero

Cuéllar

Chivato³

et al. 2004

J. Helminthol.

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Anisakis simplex crude extracts (CE) (IPI, ASAC and ALK-ABELLO), A. simplex larval antigens purified using a column of IgG anti-A. simplex (PAK) or a column of IgG anti-Ascaris suum (PAS), antigen eluted from columns of IgG anti-A. suum (EAS) and an A. suum adult CE were assayed by the skin prick test. Thirty percent of assayed patients showed a negative reaction in the Anisakis skin prick test. Of 70% positives, two patients had a weal greater than that produced by histamine with the A. simplex extract from ABELLO and IPI. The A. suum skin prick test was positive in 35% of patients, with a lower reaction than that observed with the A. simplex extract from IPI in 57% of the sera and a higher reaction in 28% of the sera. All patients with positive reactions with the crude extract also showed positive weals with the two purified antigens, PAK and PAS. All patients, except three, with a reaction to A. suum antigen, were positive to the EAS antigen. In five patients the weal size produced by PAS was greater than that observed with PAK, whereas in another six patients the contrary was observed. Only one of these six patients did not react to EAS antigen, coincident with the patient showing only a slight increase (7%) in the weal size induced by PAK vs. PAS. When the EAS antigen was tested on patients positive to both PAK and PAS, six patients presented a weal size of >30% and only three patients who were positive to PAS did not react to the EAS antigen. These three patients were also negative against the A. suum CE. Purification by affinity chromatography eliminates from the PAS antigen the proteins responsible for producing cross-reactions with Ascaris (present in the EAS antigen).

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An Immunochemical Investigation of the Allergens from <i>Ascaris suum </i>Perienteric Fluid

Cited by 7 publications

References 17 publications

Isolation of <i>Ascaris suum</i> Components Which Suppress IgE Antibody Responses

Isolation of <i>Ascaris suum</i> Components Which Suppress IgE Antibody Responses

Ascaris and Allergy

Evaluation by the skin prick test of Anisakis simplex antigen purified by affinity chromatography in patients clinically diagnosed with Anisakis sensitization

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