An immunoassay for plasma cortisol based on chemiluminescence

Kohen, F.; Pazzagli, Mario; Kim, J.B.; Lindner, H. R.

doi:10.1016/0039-128x(80)90030-6

Cited by 48 publications

(10 citation statements)

References 16 publications

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“…The sensitivity analysis did not reveal any material difference in overall percentage of adrenal insufficiency if only articles using a RIA were included, or if only articles using the ACTH250g test were included. Although the diagnostic performance of RIA in the routine evaluation of adrenocortical function is considered superior to other competitive-protein-binding analytical methods, like fluorimetry (91,92), the chemiluminescence immunoassay seems to have comparable diagnostic performance and accuracy to RIA (93). It should be kept in mind that test criteria for adrenal insufficiency available in clinical practice have a high sensitivity rather than a high specificity and therefore the number of false positive test results is not negligible.…”

Section: Discussionmentioning

confidence: 97%

Adrenal Insufficiency in Corticosteroids Use: Systematic Review and Meta-Analysis

Broersen

Pereira

Jørgensen

et al. 2015

The Journal of Clinical Endocrinology &Amp; Metabolism

345

307

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1) Adrenal insufficiency after discontinuation of glucocorticoid occurs frequently; 2) there is no administration form, dosing, treatment duration, or underlying disease for which adrenal insufficiency can be excluded with certainty, although higher dose and longer use give the highest risk; 3) the threshold to test corticosteroid users for adrenal insufficiency should be low in clinical practice, especially for those patients with nonspecific symptoms after cessation.

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Section: Discussionmentioning

confidence: 97%

Adrenal Insufficiency in Corticosteroids Use: Systematic Review and Meta-Analysis

Broersen

Pereira

Jørgensen

et al. 2015

The Journal of Clinical Endocrinology &Amp; Metabolism

345

307

View full text Add to dashboard Cite

show abstract

“…First, by using a human cortisol test method using chemiluminescence immunoassay (Kohen et al, 1980), the test sensitivity was 0.2 µg/dl. Second, by using salivary ELISA test method (Bennett and Hayssen, 2010), the sensitivity was 0.003 µg/dl.…”

Section: Methodsmentioning

confidence: 99%

Wool cortisol is a better indicator of stress than blood cortisol in ewes exposed to heat stress and water restriction

Lee

Lohakare

Son

et al. 2014

Animal

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This study investigated the effect of water restriction on wool and blood cortisol concentrations and water consumption patterns in heat-stressed sheep. Nine Corriedale female sheep (average BW = 43 ± 6.5 kg) were individually fed diets based on maintenance requirement in metabolic crates. They were assigned to three treatments according to a Latin square design (3 × 3) for three periods with a 21-day duration for each period (nine sheep per treatment). Treatments included free access to water (FAW), 2 h water restriction (2hWR) and 3 h water restriction (3hWR) after feeding. Average temperaturehumidity index in the experimental room was 27.9 throughout the experiment that defines heat stress conditions. Wool samples were taken at the end of each period on day 21. No differences were found in cortisol concentration in each fragment (dried, washed and residual extract) of wool (P < 0.05). Total wool cortisol concentration was higher in the 3hWR group than the other treatments (P < 0.05). Blood cortisol was not different among the treatments (P > 0.05) and resulted in higher variable data compared with wool cortisol. Blood neutrophils and neutrophil/lymphocyte ratio suppressed in FAW and 3hWR groups compared with the 2hWR group (P < 0.05). The duration of water consumption recorded after feeding in the 3hWR group was higher than in the 2hWR group when recorded in the afternoon (P < 0.01). Water consumption rate was higher in the 3hWR group than in the 2hWR group (P < 0.01). However, total water consumed was lower in the 3hWR group compared with other treatments (P > 0.05). It can be concluded that wool cortisol provides more precise and accurate data than blood cortisol during heat stress conditions. Water restriction for 3 h after feeding can act as a stressor and is critical for sheep during heat stress as the consumption of water decreases with restriction.

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“…Heparinized samples were centrifuged at 4000 × g for 5 minutes, and serum was collected and frozen at −80°C until the cortisol assay could be performed. Serum cortisol levels were later determined by using a solid phase, competitive chemiluminescent enzyme immunoassay as previously described …”

Section: Methodsmentioning

confidence: 99%

Influence of caudal epidural analgesia on cortisol concentrations and pain‐related behavioral responses in mares during and after ovariectomy via colpotomy

et al. 2018

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An immunoassay for plasma cortisol based on chemiluminescence

Cited by 48 publications

References 16 publications

Adrenal Insufficiency in Corticosteroids Use: Systematic Review and Meta-Analysis

Adrenal Insufficiency in Corticosteroids Use: Systematic Review and Meta-Analysis

Wool cortisol is a better indicator of stress than blood cortisol in ewes exposed to heat stress and water restriction

Influence of caudal epidural analgesia on cortisol concentrations and pain‐related behavioral responses in mares during and after ovariectomy via colpotomy

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