An immunoaffinity liquid chromatography–tandem mass spectrometry assay for detection of endogenous aggrecan fragments in biological fluids: Use as a biomarker for aggrecanase activity and cartilage degradation

Dufield, Dawn; Nemirovskiy, Olga V.; Jennings, Michael J.; Tortorella, Micky D.; Malfait, Anne‐Marie; Mathews, W R

doi:10.1016/j.ab.2010.06.044

Cited by 46 publications

(31 citation statements)

References 28 publications

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“…31 In a canine model of posttraumatic OA, serum level of KS and HA rise between 1 to 3 weeks after ACLT. 56 In ACLT dog model, tenascin-C (TN-C), an extracellular matrix glycoprotein, increased in synovial fluid 1 month after ACLT compared with the control group.…”

Section: Dogsmentioning

confidence: 99%

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Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

et al. 2016

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Objective. Osteoarthritis (OA) is one of the leading causes of disability within the adult population. Currently, its diagnosis is mainly based on clinical examination and standard radiography. To date, there is no way to detect the disease at a molecular level, before the appearance of structural changes and symptoms. So an attractive alternative for monitoring OA is the measurement of biochemical markers in blood, urine, or synovial fluid, which could reflect metabolic changes in joint tissue and therefore disease onset and progression. Animal models are relevant to investigate the early stage of OA and metabolic changes occurring in joint tissues. The goal of this narrative review is to summarize the scientific data available in the literature on soluble biomarkers in animal models of OA. Design. A literature search was conducted using the PubMed/ Medline and Scopus databases between February 1995 and December 2015. All original articles, systematic and narrative reviews published in French or in English were considered. Results. We summarized the data of 69 studies and proposed a classification scheme for OA biomarkers in animal studies, largely inspired by the BIPEDS classification. Conclusions. Studies about biomarkers and animal models indicate that some markers could be valuable to monitor OA progression and assess therapeutic response in some animal models.

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Section: Dogsmentioning

confidence: 99%

“…31 Moreover, synovial fluid ARGS levels increased following surgically induced joint instability in the rat meniscal tear (MT) model.…”

Section: Ratsmentioning

confidence: 99%

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

et al. 2016

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“…The synthesis of extracellular matrix of aggrecan and collagen type II was being investigated for their role in cartilage formation [5,30,31]. The OA+ESWT group showed significantly increased amount of the collagen type II (about 3 fold higher) and aggrecan (about 20 fold higher) at 2 and 4 weeks when compared with the NC and OA group (P<0.05).…”

Section: The Effect Of Eswt On Pdia-3 Expression and Extracellular Mamentioning

confidence: 99%

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Hsu

Cheng

Wang

et al. 2016

Preprint

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Dysregulation of cartilage homeostasis and the changes in the density and the architecture of the subchondral bone were postulated as a potent mechanically pathological activity contributing to osteoarthritis (OA) pathogenesis. Extracorporeal shockwave therapy (ESWT) is a new, none invasive and effective method in the treatment of animal OA model. In the current study, we demonstrated that shockwave induced the expression of protein-disulfide isomerase-associated 3 (Pdia-3) which is a multifunctional protein hypothesized to be a significant mediator for 1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3) signaling pathway using two-dimensional electrophoresis. Histological analysis and quantitative polymerase chain reaction (qPCR) were verified and observed that the expression of Pdia-3 at 2 weeks was significantly higher than that of any other group at 4 weeks, 8 weeks, and 12 weeks post-shockwave treatment in early OA knee of rat. The other factors of the 1α,25(OH)2D3 rapid membrane signaling pathway including extracellular signal-regulated protein kinases 1 (ERK1), osteopontin (OPG), alkaline phosphatase (ALP), and matrix metallopeptidase 13 (MMP13) were measured and significantly increased by qPCR at 2 weeks post-shockwave treatment in early OA knee. Our proteomic data revealed significant Pdia-3 expression in microenvironments of joint tissue that could be actively responded to ESWT, which may potentially regulate biological function of chondrocytes and osteoblasts in the treatment of OA knee.

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“…Progressive versus non progressive OA SELDI 300 peaks APOC-I, APOC-III, TTHY fragment [47] OA K/L I/II versus K/L IV versus HC iTRAQ + LC-MS/MS 349 26 differential proteins [8] OA versus RA versus HC autoAb profiling Antigen and NAPPA arrays 3840 antigens Anti-CHST14 auto-antibodies [48] Serum and synovial fluid OA versus HC UF + LC-MS/MS 40 COL2, PRG4, SAA, TUB, VIME, MGP [50] MB + MALDI-TOF 61 peaks Two peptide peaks [55] Early and late OA versus HC 1-DE + LC-MS/MS 135 18 altered in OA [51] DIGE + SRM 337 AFAM, CLUS, LUMI, PEDF, ALS [56] PsA versus early OA LC-MS/MS + SRM 12 proteins quantified by SRM Apart from serum or SF, no other MS-based studies have been carried out for OA biomarker discovery in alternative body fluids, with the exception of the development of an immunoaffinity-LC-MS/MS assay for the determination of a type II collagen neoepitope in urine [58,59]. Being a promising strategy for the accurate quantification of markers in biofluids, this approach was more recently applied for the analysis of endogenous aggrecan fragments in SF and urine [60].…”

Section: Proteomic Analysis Of Secretomes From Joint Tissues and Cellsmentioning

confidence: 99%

Lessons from the proteomic study of osteoarthritis

Ruiz-Romero

Fernández-Puente

Calamia

et al. 2015

Expert Review of Proteomics

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Osteoarthritis is the most common rheumatic pathology and one of the leading causes of disability worldwide. It is a very complex disease whose etiopathogenesis is not fully understood. Furthermore, there are serious limitations for its management, since it lacks specific and sensitive biomarkers for early diagnosis, prognosis and therapeutic monitoring. Proteomic approaches performed in the last few decades have contributed to the knowledge on the molecular mechanisms that participate in this pathology and they have also led to interesting panels of putative biomarker candidates. In the next few years, further efforts should be made for translating these findings into the clinical routines. It is expected that targeted proteomics strategies will be highly valuable for the verification and qualification of biomarkers of osteoarthritis.

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An immunoaffinity liquid chromatography–tandem mass spectrometry assay for detection of endogenous aggrecan fragments in biological fluids: Use as a biomarker for aggrecanase activity and cartilage degradation

Cited by 46 publications

References 28 publications

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Lessons from the proteomic study of osteoarthritis

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An immunoaffinity liquid chromatography–tandem mass spectrometry assay for detection of endogenous aggrecan fragments in biological fluids: Use as a biomarker for aggrecanase activity and cartilage degradation

Cited by 46 publications

References 28 publications

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1&alpha;,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Lessons from the proteomic study of osteoarthritis

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ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee