An Immune Adherence Assay for Discrimination Between Etiologic Agents of Hemorrhagic Fever with Renal Syndrome

Abstract

Antigens of the viruses that cause hemorrhagic fever with renal syndrome (HFRS) and antibodies to these antigens were titrated in an immune adherence hemagglutination (IAHA) test and a CF test. Vero-E6 cells infected with the SR-11 strain of HFRS virus, an isolate from rats associated with a laboratory outbreak in Japan, and the 76-118 strain of Hantaan virus, the etiologic agent of Korean hemorrhagic fever, were used as antigens in the IAHA, CF, and indirect immunofluorescent antibody (IFA) tests. Sera from patients with different types of HFRS were tested against both strains of virus. Titers of antibody detected by the IFA test were similar for the two strains, but the IAHA test discriminated between antibodies to the two types of HFRS virus. Antigenic differences between HFRS viruses were suggested by the results of the IAHA test.

Four Serotypes of Haemorrhagic Fever with Renal Syndrome Viruses Identified by Polyclonal and Monoclonal Antibodies

“…Fluorescein isothiocyanate conjugates of antibodies to human IgG, IgM and IgA (Hyland Laboratories, Deerfield, IU., U.S.A.), rat IgG and mouse IgG, IgM and IgA (Cappel Laboratories) were used. IAHA tests were carried out in U-bottomed polystyrene microplates by methods described previously (Inouye et al, 1981;Sugiyama et al, 1984b). All reagents were diluted with veronal-buffered NaCI (pH 7.3) with Ca 2+, Mg 2÷, 0.1 ~ bovine serum albumin, 0.002~ gelatin, and 0.02~ NAN3.…”

“…Studies on the serotyping of HFRS viruses have been reported by Goldgaber et al (1985) and Lee et al (1985 b). We have developed an immune adherence haemagglutination (IAHA) test for the detection of HFRS virus antigen and antibody (Sugiyama et al, 1984b), and demonstrated that it discriminates Rattus virus strains from the Apodemus strain 76-118 (Sugiyama et al, 1984a). In the present study, further serological analyses of 20 representative strains of HFRS virus isolated in Korea, China, Japan, the U.S.S.R., Finland and the U.S.A. from different rodent species were carried out using polyclonal and monoclonal antibodies to clarify the antigenic relationships of HFRS viruses.…”

The Hantaviruses, Etiologic Agents of Hemorrhagic Fever with Renal Syndrome: A Possible Cause of Hypertension and Chronic Renal Disease in the United States

“…Standard serological tests to confirm a diagnosis of HFRS are mostly based on immunofluorescent or enzyme-linked im munosorbent assays to detect immunoglobulin G (29, 64,67,69,90). However, for epidemiological monitoring in regions where more than one hantavirus circulates, positive identification of the infecting agent may de pend on cumbersome cross-plaque-reduction neutralization assays of serum performed on batteries of hantaviruses under laboratory containment con ditions (91).…”

Growth Experiment of Hantaan Virus in A549 Cells: An Attempt to Improve the Immunofluorescent Antibody Technique for Hemorrhagic Fever with Renal Syndrome

“…To clarify the epidemiology and diagnosis of the disease, the detection of an antibody to the causative virus has been attempted by employing various serological methods such as immunofluorescent antibody technique (IFA) [4,7,8,11], enzyme-linked immunosorbent assay (ELISA) [6,14], and other related tests [13] . Among these methods, IFA is used most frequently because of its excellent sensitivity and ease of performance.…”