An imidazole coumarin derivative enhances the antiviral response to spring viremia of carp virus infection in zebrafish

Antiviral activity of anisomycin against spring viraemia of carp virus in epithelioma papulosum cyprini cells and zebrafish

“…Thus, Ani as an antiviral agent showed an effective protection on SVCV-infected zebrafish. Take the example of our recently synthesized coumarin derivatives, statistics showed that they increased the survival rates of zebrafish by 17.5 and 22.5% and inhibited the G mRNA expression by about 50% in kidney and spleen at the first four days Liu et al, 2019). Compared with these two coumarin derivatives, Ani with an inhibitory percentage of > 60% was more effective in the treatment of SVCV infection in zebrafish.…”

“…In addition, some researches have been conducted to develop anti-SVCV drugs. Previous studies reported that several natural products and their derivatives showed a good anti-SVCV potential Liu et al, 2017Liu et al, , 2018Liu et al, 2019;Shen et al, 2018a, b;Shen et al, 2019). These findings indicate that it is feasible to control SVCV infection by drug treatment.…”

“…Considered as one of the most serious viral threats for cyprinids, spring viraemia of carp (SVC) has caused large-scale deaths and serious economic losses in the aquaculture industry in the past decades (Ashraf et al, 2016). The causative agent of SVC is spring viraemia of carp virus (SVCV) which is classified as a member of the Rhabdoviridae family (Ashraf et al, 2016;Liu et al, 2019). SVCV is an enveloped, bullet-shaped RNA virus encoding a negative-sense, ssRNA genome.…”

Zebrafish as a Model for Fish Diseases in Aquaculture

“…Therefore, many studies focus on this molecule or other molecules influencing the production of IFN. The immunological investigations mentioned in this review have been dedicated to innate and adaptive immunity using classical transcription analyses [40][41][42][43][44]48,55,76,77,[80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95][96], gnotobiotic (germ-free) [81] and transgenic fish lines for functional studies [89,91,[97][98][99], but also visualization of the behaviour of professional phagocytes [100].…”

“…An inhibitory effect was observed for some zebrafish Irfs at lower concentrations and a synergistic effect at higher concentrations [ 92 ]. An Ifn-inducing substance, 7-(6-(2-methyl-imidazole))-coumarin (D5) was found to elicit an innate immune response in non-viral infected zebrafish by up-regulating the expression of interferon genes ( Ifnγ , Ifnφ1 , Ifnφ2 and Rig-1 ) and to inhibit SVCV replication after administration in infected fish [ 93 ]. Genes and transcription factors involved in different pathways have been identified, which were suggested to be implicated in suppression of the virus and/or host survival responses [ 55 ].…”